-
1.发明授权Enhancement of ribozyme catalytic activity by A 2'-O-substituted facilitator oligonucleotide 失效通过2'-O-取代促进剂寡核苷酸增强核酶催化活性
公开(公告)号：US6087484A
公开(公告)日：2000-07-11
申请号：US819942
申请日：1997-03-18
Applicant: John Goodchild
Inventor： John Goodchild
IPC: A61K38/00 , C07K14/16 , C12N15/113 , C12N15/11 , C12Q1/68
CPC classification number: C12N15/1132 , C07K14/005 , A61K38/00 , C12N2310/111 , C12N2310/121 , C12N2740/16022
Abstract: Methods are disclosed for increasing ribozyme catalytic activity without reducing specificity, which methods comprise contacting an RNA molecule with a ribozyme and a 2'-O-substituted facilitator oligonucleotide. The present invention further provides compositions comprising a ribozyme and an effective amount of a 2'-O-methyl substituted facilitator oligonucleotide. The use of a facilitator, particularly a 2'-O-substituted facilitator, and more especially a 2'-O-methyl substituted facilitator, greatly enhances ribozyme catalytic activity, frequently making an otherwise inactive ribozyme active.
Abstract translation: 公开了增加核酶催化活性而不降低特异性的方法，该方法包括使RNA分子与核酶和2'-O-取代的促进剂寡核苷酸接触。 本发明还提供了包含核酶和有效量的2'-O-甲基取代促进剂寡核苷酸的组合物。 使用促进剂，特别是2'-O-取代促进剂，更特别是2'-O-甲基取代促进剂，大大增强核酶催化活性，通常使否则无活性的核酶具有活性。
-
公开(公告)号：US5856459A
公开(公告)日：1999-01-05
申请号：US468352
申请日：1995-06-06
Applicant: Bruce L. Frank , Peter C. Roberts , John Goodchild , J. Charles Craig , John S. Mills
Inventor： Bruce L. Frank , Peter C. Roberts , John Goodchild , J. Charles Craig , John S. Mills
IPC: C12N15/113 , C07H21/00
CPC classification number: C12N15/1131 , C12N2310/313 , C12N2310/314 , C12N2310/315
Abstract: The present invention discloses synthetic oligonucleotides complementary to contiguous and noncontiguous regions of the HBV RNA. Also disclosed are methods and kits for inhibiting the replication and expression of HBV, and for treating HBV infections and associated conditions.
-
3.发明授权Method of using ribozyme analogs having rigid, non-nucleotidic molecular linkers 失效使用具有刚性非核酸分子接头的核酶类似物的方法
公开(公告)号：US5679555A
公开(公告)日：1997-10-21
申请号：US478940
申请日：1995-06-07
Applicant: John Goodchild , Thomas E. Leonard
Inventor： John Goodchild , Thomas E. Leonard
IPC: C12N15/09 , A61K31/70 , A61K38/00 , A61K48/00 , C07H21/02 , C12N9/00 , C12N15/113 , C07H21/00 , C12P19/34 , C12Q1/68
CPC classification number: C12N15/113 , A61K38/00 , C12N2310/121 , C12N2310/318 , C12N2310/321
Abstract: Disclosed are ribozyme analogs having the ability to endonucleolytically cleave a sequence of 3' to 5' linked ribonucleotides. The ribozyme analogs include a plurality of 3' to 5' covalently-linked nucleotides, and a rigid molecular linker having at least one non-nucleotidic molecule covalently linked to two of the nucleotides. Also disclosed are methods of preparing and utilizing the ribozyme analogs of the invention, and pharmaceutical formulations and kits containing such ribozyme analogs.
Abstract translation: 公开了具有内切核酸切割3'至5'连接的核糖核苷酸序列的能力的核酶类似物。 核酶类似物包括多个3'至5'共价连接的核苷酸，以及具有至少一个共价连接到两个核苷酸的非核苷酸分子的刚性分子接头。 还公开了制备和利用本发明的核酶类似物的方法以及含有这种核酶类似物的药物制剂和试剂盒。
-
公开(公告)号：US5650502A
公开(公告)日：1997-07-22
申请号：US336526
申请日：1994-11-09
Applicant: John Goodchild , Thomas E. Leonard
Inventor： John Goodchild , Thomas E. Leonard
IPC: C12N15/09 , A61K31/70 , A61K38/00 , A61K48/00 , C07H21/02 , C12N9/00 , C12N15/113 , C07H21/00 , C07F9/02 , C07H21/04 , C12Q1/68
CPC classification number: C12N15/113 , A61K38/00 , C12N2310/121 , C12N2310/318 , C12N2310/321
Abstract: Disclosed are ribozyme analogs having the ability to endonucleolytically cleave a sequence of 3' to 5' linked ribonucleotides. The ribozyme analogs include a plurality of 3' to 5' covalently-linked nucleotides, and a rigid molecular linker having at least one non-nucleotidic molecule covalently linked to two of the nucleotides. Also disclosed are methods of preparing and utilizing the ribozyme analogs of the invention, and pharmaceutical formulations and kits containing such ribozyme analogs.
Abstract translation: 公开了具有内切核酸切割3'至5'连接的核糖核苷酸序列的能力的核酶类似物。 核酶类似物包括多个3'至5'共价连接的核苷酸，以及具有至少一个共价连接到两个核苷酸的非核苷酸分子的刚性分子接头。 还公开了制备和利用本发明的核酶类似物的方法以及含有这种核酶类似物的药物制剂和试剂盒。
-
公开(公告)号：US5646021A
公开(公告)日：1997-07-08
申请号：US477883
申请日：1995-06-07
Applicant: John Goodchild , Thomas E. Leonard
Inventor： John Goodchild , Thomas E. Leonard
CPC classification number: C12N15/113 , A61K38/00 , A61K48/00 , C12N2310/111 , C12N2310/12 , C12N2310/121 , C12N2310/3125 , C12N2310/313 , C12N2310/318
Abstract: Disclosed is a finderon having the ability to endonucleolytically cleave a sequence of 3' to 5' linked ribonucleotides. The finderon includes a rigid linker comprising at least one non-nucleotidic unit, flanked by first and second flanking regions of at least four contiguous, covalently-linked nucleotides. At least a portion of each flanking region is complementary to a target region on a substrate RNA molecule. Also disclosed are methods of preparing and using a finderon, and kits including a finderon.
-
Patent Agency Ranking6.发明授权Enhancement of ribozyme catalytic activity by a neighboring facilitator oligonucleotide 失效通过相邻促进剂寡核苷酸增强核酶催化活性
公开(公告)号：US5612469A
公开(公告)日：1997-03-18
申请号：US431625
申请日：1995-05-01
Applicant: John Goodchild
Inventor： John Goodchild
IPC: A61K38/00 , C07K14/16 , C12N15/113 , C12N15/11 , C12Q1/68
CPC classification number: C12N15/1132 , C07K14/005 , A61K38/00 , C12N2310/111 , C12N2310/121 , C12N2740/16022
Abstract: Methods are disclosed for increasing ribozyme catalytic activity without reducing specificity, which methods comprise contacting an RNA molecule with a ribozyme and a facilitator oligonucleotide.The present invention further provides compositions comprising a ribozyme and an effective amount of a facilitator oligonucleotide.
Abstract translation: 公开了用于增加核酶催化活性而不降低特异性的方法，该方法包括使RNA分子与核酶和促进剂寡核苷酸接触。 本发明还提供了包含核酶和有效量的辅助性寡核苷酸的组合物。
-
公开(公告)号：US4806463A
公开(公告)日：1989-02-21
申请号：US867231
申请日：1986-05-23
Applicant: John Goodchild , Paul C. Zamecnik
Inventor： John Goodchild , Paul C. Zamecnik
IPC: C07H21/00 , C12N15/113 , C12Q1/70 , G01N33/569 , A61K39/12 , C12Q1/02
CPC classification number: A61K31/70 , C07H21/00 , C12N15/1131 , C12N15/1132 , C12Q1/701 , C12Q1/703 , G01N33/56988 , C12N2310/315
Abstract: Inhibition of HTLV-III by adminstration of an oligonucleotide complementary to highly conserved regions of the HTLV-III genome necessary for HTLV-III replication and/or gene expression is described, as are oligodeoxynucleotide sequences which are complementary to those regions, methods of inhibiting HTLV-III replication and gene expression and methods of determining the presence or absence of HTLV-III virus in samples such as blood, saliva, urine and tears.
Abstract translation: 描述了与HTLV-III复制和/或基因表达所必需的HTLV-III基因组的高度保守区域互补的寡核苷酸对HTLV-III的抑制，以及与这些区域互补的寡脱氧核苷酸序列，抑制HTLV的方法 -III复制和基因表达以及在血液，唾液，尿液和眼泪等样品中确定HTLV-III病毒的存在或不存在的方法。
-
公开(公告)号：US5679554A
公开(公告)日：1997-10-21
申请号：US472427
申请日：1995-06-07
Applicant: John Goodchild , Thomas E. Leonard
Inventor： John Goodchild , Thomas E. Leonard
CPC classification number: C12N15/113 , A61K38/00 , A61K48/00 , C12N2310/111 , C12N2310/12 , C12N2310/121 , C12N2310/3125 , C12N2310/313 , C12N2310/318
Abstract: Disclosed is a finderon having the ability to endonucleolytically cleave a sequence of 3' to 5' linked ribonucleotides. The finderon includes a rigid linker comprising at least one non-nucleotidic unit, flanked by first and second flanking regions of at least four contiguous, covalently-linked nucleotides. At least a portion of each flanking region is complementary to a target region on a substrate RNA molecule. Also disclosed are methods of preparing and using a finderon, and kits including a finderon.
Abstract translation: 公开了具有内切核酸切割3'至5'连接的核糖核苷酸序列的能力的检测器。 取样器包括包含至少一个非核苷酸单元的刚性接头，其侧翼为至少四个相邻的共价连接的核苷酸的第一和第二侧翼区域。 每个侧翼区域的至少一部分与底物RNA分子上的靶区域互补。 还公开了制备和使用检测器的方法，以及包括检测器的试剂盒。
-
公开(公告)号：US5545729A
公开(公告)日：1996-08-13
申请号：US361687
申请日：1994-12-22
Applicant: John Goodchild , Steven M. Nesbitt
Inventor： John Goodchild , Steven M. Nesbitt
CPC classification number: C12N15/113 , C12N2310/121 , C12N2310/3125 , C12N2310/315 , C12N2310/321
Abstract: Disclosed are stabilized ribozyme analogs having the ability to endonucleolytically cleave a sequence of 3' to 5' linked ribonucleotides. These ribozyme analogs include modifications at specific loop, catalytic core, and flanking region nucleotides which makes them more resistant to nucleases. Also disclosed are methods of preparing and utilizing the ribozyme analogs of the invention, and pharmaceutical formulations and kits containing such ribozyme analogs.
Abstract translation: 公开了具有内切核酸切割3'至5'连接的核糖核苷酸序列的能力的稳定的核酶类似物。 这些核酶类似物包括在特定环上的修饰，催化核心和侧翼区核苷酸，这使得它们对核酸酶更具抗性。 还公开了制备和利用本发明的核酶类似物的方法以及含有这种核酶类似物的药物制剂和试剂盒。
-
公开(公告)号：US635128A
公开(公告)日：1899-10-17
申请号：US1899709254
申请日：1899-03-16
Applicant: JOHN GOODCHILD , DISSOWAY CROWELL M
Inventor： DISSOWAY CROWELL M
CPC classification number: B60B17/0055 , Y10S48/01
-
-
-
-
-
-
-
-
-
Search Results
26
records
(took 0.013 seconds)
