公开(公告)号：US06303354B1

公开(公告)日：2001-10-16

申请号：US09721259

申请日：2000-11-21

申请人： Walter Weyler ,  Timothy C. Dodge ,  John J. Lauff ,  Dan J. Wendt

发明人： Walter Weyler ,  Timothy C. Dodge ,  John J. Lauff ,  Dan J. Wendt

IPC分类号： C12P100

CPC分类号： H04W24/00 ,  C12N9/0069 ,  C12N9/86 ,  C12N15/52 ,  C12P17/165 ,  H04W84/14

摘要： There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.

摘要翻译： 提供了用于靛蓝的生物合成生产的改进方法，其改进包括从其中产生这种靛蓝的肉汤中除去不需要的副产物如靛红或靛玉红。 可以通过酶促活性使用靛红去除酶如靛红水解酶或通过其它技术如加工参数（升高的温度，pH）或通过使含有靛红的培养液与适当的吸附化合物/组合物 碳或合适的树脂。 由于靛红是靛玉红的前体，因此靛玉红水平因靛红去除而降低。

公开(公告)号：US5866396A

公开(公告)日：1999-02-02

申请号：US763919

申请日：1996-12-11

申请人： Walter Weyler ,  Timothy C. Dodge ,  John J. Lauff ,  Dan J. Wendt

发明人： Walter Weyler ,  Timothy C. Dodge ,  John J. Lauff ,  Dan J. Wendt

IPC分类号： C12N15/09 ,  C09B7/00 ,  C12N1/21 ,  C12N9/02 ,  C12N9/86 ,  C12N15/52 ,  C12N15/53 ,  C12N15/55 ,  C12P17/16 ,  C12P17/18 ,  C12R1/19 ,  H04W24/00 ,  H04W84/14 ,  C12N9/14 ,  C07H21/04

CPC分类号： H04W24/00 ,  C12N15/52 ,  C12N9/0069 ,  C12N9/86 ,  C12P17/165 ,  H04W84/14

摘要： There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.

摘要翻译： 提供了用于靛蓝的生物合成生产的改进方法，其改进包括从其中产生这种靛蓝的肉汤中除去不需要的副产物如靛红或靛玉红。 可以通过酶促活性使用靛红去除酶如靛红水解酶或通过其它技术如加工参数（升高的温度，pH）或通过使含有靛红的培养液与适当的吸附化合物/组合物 碳或合适的树脂。 由于靛红是靛玉红的前体，因此靛玉红水平因靛红去除而降低。

公开(公告)号：US06190892B1

公开(公告)日：2001-02-20

申请号：US08560729

申请日：1995-11-20

申请人： Walter Weyler ,  Timothy C. Dodge ,  John J. Lauff ,  Dan J. Wendt

发明人： Walter Weyler ,  Timothy C. Dodge ,  John J. Lauff ,  Dan J. Wendt

IPC分类号： C07H2104

CPC分类号： H04W24/00 ,  C12N9/0069 ,  C12N9/86 ,  C12N15/52 ,  C12P17/165 ,  H04W84/14

摘要： There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.