公开(公告)号：US07074905B2

公开(公告)日：2006-07-11

申请号：US09766378

申请日：2001-01-19

Applicant: Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

IPC: C07K16/00 ,  C07K14/74

CPC classification number: C07K14/70539 ,  A61K38/00 ,  C07K2319/00 ,  C12N2799/026

Abstract: The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to single chain MHC class II complexes that include a class II β2 chain modification, e.g., deletion of essentially the entire class II β2 chain. In another aspect, the invention features single chain MHC class II which comprise an immunoglobin constant chain or fragment. Further provided are polyspecific MHC complexes comprising at least one single chain MHC class II molecule. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

公开(公告)号：US06232445B1

公开(公告)日：2001-05-15

申请号：US08960190

申请日：1997-10-29

Applicant: Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

IPC: C12P2108

CPC classification number: C07K14/70539 ,  A61K38/00 ,  C07K2319/00 ,  C12N2799/026

Abstract: The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to single chain MHC class II complexes that include a class II &bgr;2 chain modification, e.g., deletion of essentially the entire class II &bgr;2 chain. In another aspect, the invention features single chain MHC class II which comprise an immunoglobin constant chain or fragment. Further provided are polyspecific MHC complexes comprising at least one single chain MHC class II molecule. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

Abstract translation: 本发明涉及主要组氨酸复合物（MHC）分子的新型复合物和这种复合物的用途。 一方面，本发明涉及包含II类β2链修饰的单链MHC II类复合物，例如基本上缺失整个II类β2链。 另一方面，本发明的特征在于包含免疫球蛋白恒定链或片段的单链MHC II类。 还提供了包含至少一种单链MHC II类分子的多特异性MHC复合物。 本发明的MHC复合物可用于多种应用，包括：1）用于鉴定和分离调节所选T细胞活性的肽的体外筛选，包括作为T细胞受体拮抗剂和部分激动剂的肽，以及2） 抑制或诱导哺乳动物的免疫应答。