公开(公告)号：US5869270A

公开(公告)日：1999-02-09

申请号：US596387

申请日：1996-01-31

Applicant: Peter R. Rhode ,  Jin-An Jiao ,  Martin Burkhardt ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jin-An Jiao ,  Martin Burkhardt ,  Hing C. Wong

IPC: A01K67/027 ,  A61K35/76 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  A61P3/10 ,  A61P21/04 ,  A61P25/28 ,  A61P29/00 ,  A61P37/02 ,  A61P37/06 ,  A61P37/08 ,  A61P43/00 ,  C07K14/705 ,  C07K14/74 ,  C07K19/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/62 ,  C12N15/63 ,  C12N15/70 ,  C12N15/79 ,  C12P21/04 ,  C12Q1/02 ,  G01N33/53

CPC classification number: C07K14/70539 ,  A01K2217/05 ,  A61K2039/53 ,  A61K2039/6043 ,  A61K2039/622 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  C07K2319/00 ,  C07K2319/02

Abstract: The present invention relates to novel complexes of major histocompatibility complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to loaded MHC complexes that include at least one MHC molecule with a peptide-binding groove and a presenting peptide non-covalently linked to the MHC protein. In another aspect, the invention features single chain MHC class II peptide fusion complexes with a presenting peptide covalently linked to the peptide binding grove of the complex. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

Abstract translation: 本发明涉及主要组织相容性复合物（MHC）分子的新型复合物和这种复合物的用途。 一方面，本发明涉及包含至少一个具有肽结合槽的MHC分子和与MHC蛋白非共价连接的呈递肽的负载的MHC复合物。 另一方面，本发明的特征在于具有与复合物的肽结合树共价连接的呈递肽的单链MHC II类肽融合复合物。 本发明的MHC复合物可用于多种应用，包括：1）用于鉴定和分离调节所选T细胞活性的肽的体外筛选，包括作为T细胞受体拮抗剂和部分激动剂的肽，以及2） 抑制或诱导哺乳动物的免疫应答。

公开(公告)号：US07141656B2

公开(公告)日：2006-11-28

申请号：US09848164

申请日：2001-05-03

Applicant: Peter R. Rhode ,  Jin-An Jiao ,  Martin Burkhardt ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jin-An Jiao ,  Martin Burkhardt ,  Hing C. Wong

IPC: C07K16/46 ,  C07K14/74

CPC classification number: C07K14/70539 ,  A01K2217/05 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/6043 ,  A61K2039/622 ,  C07K2319/00 ,  C07K2319/02

Abstract: The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to loaded MHC complexes that include at least one MHC molecule with a peptide-binding groove and a presenting peptide non-covalently linked to the MHC protein. In another aspect, the invention features single chain MHC class II peptide fusion complexes with a presenting peptide covalently linked to the peptide binding grove of the complex. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

公开(公告)号：US07074904B2

公开(公告)日：2006-07-11

申请号：US09900379

申请日：2001-07-06

Applicant: Hing C. Wong ,  Peter R. Rhode ,  Jon A. Weidanz ,  Susan Grammer ,  Ana C. Edwards ,  Pierre-Andre Chavaillaz ,  Jin-An Jiao

Inventor： Hing C. Wong ,  Peter R. Rhode ,  Jon A. Weidanz ,  Susan Grammer ,  Ana C. Edwards ,  Pierre-Andre Chavaillaz ,  Jin-An Jiao

IPC: C07K7/00 ,  C07K14/74 ,  C07K17/02

CPC classification number: C07K14/705 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  C07K14/70532 ,  C07K14/70539 ,  C07K14/77 ,  C07K2319/00 ,  C12N2799/026 ,  Y10S530/868

Abstract: The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In particular, the invention relates to MHC fusion complexes that contain a MHC molecule with a peptide-binding groove and a presenting peptide covalently linked to the MHC protein. Fusion complexes of the invention are useful for a variety of applications including in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, methods of suppressing an immune response of a mammal and methods for inducing an immune response in a mammal.

公开(公告)号：US06306605B1

公开(公告)日：2001-10-23

申请号：US09496311

申请日：2000-02-01

Applicant: Jorge L. Acevedo ,  Peter R. Rhode

Inventor： Jorge L. Acevedo ,  Peter R. Rhode

IPC: C12Q168

CPC classification number: C12N15/67 ,  C07K16/2896 ,  C07K2317/24 ,  C07K2319/00 ,  C12N15/65

Abstract: Disclosed are methods for producing recombinant cells and especially recombinant mammalian cell lines with enhanced expression of an amino acid sequence. Also disclosed are recombinant mammalian cell lines producing high levels of the amino acid sequence. The methods and recombinant cell lines of the invention have a number of useful applications including use in the efficient and large-scale production of recombinant proteins and polypeptides.

Abstract translation: 公开了用于产生重组细胞的方法，特别是具有增强的氨基酸序列表达的重组哺乳动物细胞系。 还公开了产生高水平氨基酸序列的重组哺乳动物细胞系。 本发明的方法和重组细胞系具有许多有用的应用，包括用于有效和大规模生产重组蛋白和多肽的应用。

公开(公告)号：US6156514A

公开(公告)日：2000-12-05

申请号：US204979

申请日：1998-12-03

Applicant: Jorge L. Acevedo ,  Peter R. Rhode

Inventor： Jorge L. Acevedo ,  Peter R. Rhode

IPC: C12N15/09 ,  C07K16/28 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/65 ,  C12N15/67 ,  C12P21/02 ,  C12Q1/68

CPC classification number: C12N15/67 ,  C07K16/2896 ,  C12N15/65 ,  C07K2317/24 ,  C07K2319/00

Abstract: Disclosed are methods for producing recombinant cells and especially recombinant mammalian cell lines with enhanced expression of an amino acid sequence. Also disclosed are recombinant mammalian cell lines producing high levels of the amino acid sequence. The methods and recombinant cell lines of the invention have a number of useful applications including use in the efficient and large-scale production of recombinant proteins and polypeptides.

Abstract translation: 公开了用于产生重组细胞的方法，特别是具有增强的氨基酸序列表达的重组哺乳动物细胞系。 还公开了产生高水平氨基酸序列的重组哺乳动物细胞系。 本发明的方法和重组细胞系具有许多有用的应用，包括用于有效和大规模生产重组蛋白和多肽的应用。

公开(公告)号：US5516637A

公开(公告)日：1996-05-14

申请号：US258026

申请日：1994-06-10

Applicant: Grace P. Huang ,  Peter R. Rhode ,  Jeffrey R. Stinson ,  Hing C. Wong

Inventor： Grace P. Huang ,  Peter R. Rhode ,  Jeffrey R. Stinson ,  Hing C. Wong

IPC: G01N33/53 ,  C07K14/245 ,  C07K16/00 ,  C07K19/00 ,  C12N1/21 ,  C12N15/09 ,  C12N15/10 ,  C12N15/70 ,  C12P21/04 ,  C12Q1/02 ,  C12Q1/70 ,  C12R1/19 ,  C40B40/02 ,  G01N33/569 ,  C12Q1/68 ,  G01N33/554

CPC classification number: C40B40/02 ,  C07K14/245 ,  C07K16/00 ,  C12N15/1037 ,  C12N15/70 ,  C12Q1/02 ,  C12Q1/70 ,  C07K2319/00 ,  C07K2319/02

Abstract: The present invention relates to a fusion protein, comprising a pilin protein or a portion thereof and a heterologous polypeptide (target protein). In a preferred embodiment it relates to a method for displaying the target protein on the outer surface of a bacterial host cell capable of forming pilus. In certain embodiments, it is desirable that the pilus is a receptor for bacteriophage attachment and infection. The F pilus is preferred.

Abstract translation: 本发明涉及一种融合蛋白，其包含比林蛋白或其部分和异源多肽（靶蛋白）。 在一个优选实施方案中，其涉及在能够形成菌毛的细菌宿主细胞的外表面上显示靶蛋白的方法。 在某些实施方案中，希望菌毛是噬菌体附着和感染的受体。 F菌毛是优选的。

公开(公告)号：US07074905B2

公开(公告)日：2006-07-11

申请号：US09766378

申请日：2001-01-19

Applicant: Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

IPC: C07K16/00 ,  C07K14/74

CPC classification number: C07K14/70539 ,  A61K38/00 ,  C07K2319/00 ,  C12N2799/026

Abstract: The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to single chain MHC class II complexes that include a class II β2 chain modification, e.g., deletion of essentially the entire class II β2 chain. In another aspect, the invention features single chain MHC class II which comprise an immunoglobin constant chain or fragment. Further provided are polyspecific MHC complexes comprising at least one single chain MHC class II molecule. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

公开(公告)号：US06232445B1

公开(公告)日：2001-05-15

申请号：US08960190

申请日：1997-10-29

Applicant: Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jorge Acevedo ,  Martin Burkhardt ,  Jin-an Jiao ,  Hing C. Wong

IPC: C12P2108

CPC classification number: C07K14/70539 ,  A61K38/00 ,  C07K2319/00 ,  C12N2799/026

Abstract: The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to single chain MHC class II complexes that include a class II &bgr;2 chain modification, e.g., deletion of essentially the entire class II &bgr;2 chain. In another aspect, the invention features single chain MHC class II which comprise an immunoglobin constant chain or fragment. Further provided are polyspecific MHC complexes comprising at least one single chain MHC class II molecule. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

Abstract translation: 本发明涉及主要组氨酸复合物（MHC）分子的新型复合物和这种复合物的用途。 一方面，本发明涉及包含II类β2链修饰的单链MHC II类复合物，例如基本上缺失整个II类β2链。 另一方面，本发明的特征在于包含免疫球蛋白恒定链或片段的单链MHC II类。 还提供了包含至少一种单链MHC II类分子的多特异性MHC复合物。 本发明的MHC复合物可用于多种应用，包括：1）用于鉴定和分离调节所选T细胞活性的肽的体外筛选，包括作为T细胞受体拮抗剂和部分激动剂的肽，以及2） 抑制或诱导哺乳动物的免疫应答。

公开(公告)号：US06309645B1

公开(公告)日：2001-10-30

申请号：US09067615

申请日：1998-04-28

Applicant: Peter R. Rhode ,  Jin-An Jiao ,  Martin Burkhardt ,  Hing C. Wong

Inventor： Peter R. Rhode ,  Jin-An Jiao ,  Martin Burkhardt ,  Hing C. Wong

IPC: A61K3900

CPC classification number: C07K14/70539 ,  A01K2217/05 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/6043 ,  A61K2039/622 ,  C07K2319/00 ,  C07K2319/02

Abstract: The present invention relates to novel complexes of major histocomability complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to loaded MHC complexes that include at least one MHC molecule with a peptide-binding groove and a presenting peptide non-covalently linked to the MHC protein. In another aspect, the invention features single chain MHC class II peptide fusion complexes with a presenting peptide covalently linked to the peptide binding grove of the complex. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.

Abstract translation: 本发明涉及主要组织学复合物（MHC）分子的新型复合物和这种复合物的用途。 一方面，本发明涉及包含至少一个具有肽结合槽的MHC分子和与MHC蛋白非共价连接的呈递肽的负载的MHC复合物。 另一方面，本发明的特征在于具有与复合物的肽结合树共价连接的呈递肽的单链MHC II类肽融合复合物。 本发明的MHC复合物可用于多种应用，包括：1）用于鉴定和分离调节所选T细胞活性的肽的体外筛选，包括作为T细胞受体拮抗剂和部分激动剂的肽，以及2） 抑制或诱导哺乳动物的免疫应答。