公开(公告)号：US09517277B2

公开(公告)日：2016-12-13

申请号：US14128238

申请日：2012-07-11

申请人： Shanta Dhar ,  Joshua Choi ,  Sean Marrache

发明人： Shanta Dhar ,  Joshua Choi ,  Sean Marrache

IPC分类号： A61K51/00 ,  A61M36/14 ,  A61K47/48 ,  A61K41/00

CPC分类号： A61K47/48915 ,  A61K41/0071

摘要： Provides is a therapeutic technology that combines the phototoxic and immune-stimulating ability of photodynamic therapy with the widespread effectiveness of the immune system to reduce the viability of such as cancer cells and tumors. The nanoparticle compositions of the disclosure combine an immunostimulant with a photosensitizer using a nanoparticle delivery platform. For example, zinc pthalocyanine, which is a long-wavelength absorbing photosensitizer, integrated into a polymeric nanoparticle core made up of poly(D,L-lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG). The outside surface of the core can be coated with metallic nanoparticles, which are then modified with CpG-ODN. Metastatic mouse breast carcinoma cells showed significant photocytotoxicity of the hybrid after irradiation with a 660 nm LASER light and this activity was remarkably better than either treatment alone. Treatment of mouse bone marrow derived dendritic cells with the photodynamic therapy-killed 4T1 cell lysate showed that the combination of photodynamic therapy with a synergistic immunostimulant in a single nanoparticle system resulted in an immune response suitable for the treatment of such as a metastatic cancer.

摘要翻译： 提供一种治疗技术，将光动力疗法的光毒性和免疫刺激能力与免疫系统的广泛功效结合起来，以降低癌细胞和肿瘤的生存能力。 本公开的纳米颗粒组合物使用纳米颗粒递送平台将免疫刺激剂与光敏剂组合。 例如，作为长波长吸收光敏剂的锌酞菁聚合在由聚（D，L-乳酸 - 共 - 乙醇酸）-b-聚（乙二醇）（PLGA-b- PEG）。 芯的外表面可以涂覆金属纳米粒子，然后用CpG-ODN进行修饰。 转移性小鼠乳腺癌细胞在用660nm激光照射后显示杂合物的显着的光细胞毒性，并且该活性明显优于单独的任一种治疗。 用光动力治疗杀死的4T1细胞裂解物处理小鼠骨髓来源的树突状细胞显示，在单个纳米颗粒系统中光动力学治疗与协同免疫刺激剂的组合导致适合于治疗诸如转移性癌症的免疫应答。

公开(公告)号：US20140220143A1

公开(公告)日：2014-08-07

申请号：US14128238

申请日：2012-07-11

申请人： Shanta Dhar ,  Joshua Choi ,  Sean Marache

发明人： Shanta Dhar ,  Joshua Choi ,  Sean Marache

IPC分类号： A61K47/48 ,  A61K41/00

CPC分类号： A61K47/48915 ,  A61K41/0071

摘要： Provides is a therapeutic technology that combines the phototoxic and immune-stimulating ability of photodynamic therapy with the widespread effectiveness of the immune system to reduce the viability of such as cancer cells and tumors. The nanoparticle compositions of the disclosure combine an immunostimulant with a photosensitizer using a nanoparticle delivery platform. For example, zinc pthalocyanine, which is a long-wavelength absorbing photosensitizer, integrated into a polymeric nanoparticle core made up of poly(D,L-lactic-co-glycolic acid)-b-poly(ethylene glycol) (PLGA-b-PEG). The outside surface of the core can be coated with metallic nanoparticles, which are then modified with CpG-ODN. Metastatic mouse breast carcinoma cells showed significant photocytotoxicity of the hybrid after irradiation with a 660 nm LASER light and this activity was remarkably better than either treatment alone. Treatment of mouse bone marrow derived dendritic cells with the photodynamic therapy-killed 4T1 cell lysate showed that the combination of photodynamic therapy with a synergistic immunostimulant in a single nanoparticle system resulted in an immune response suitable for the treatment of such as a metastatic cancer.

摘要翻译： 提供一种治疗技术，将光动力疗法的光毒性和免疫刺激能力与免疫系统的广泛功效结合起来，以降低癌细胞和肿瘤的生存能力。 本公开的纳米颗粒组合物使用纳米颗粒递送平台将免疫刺激剂与光敏剂组合。 例如，作为长波长吸收光敏剂的锌酞菁聚合在由聚（D，L-乳酸 - 共 - 乙醇酸）-b-聚（乙二醇）（PLGA-b- PEG）。 芯的外表面可以涂覆金属纳米粒子，然后用CpG-ODN进行修饰。 转移性小鼠乳腺癌细胞在用660nm激光照射后显示杂合物的显着的光细胞毒性，并且该活性明显优于单独的任一种治疗。 用光动力治疗杀死的4T1细胞裂解物处理小鼠骨髓来源的树突状细胞显示，在单个纳米颗粒系统中光动力学治疗与协同免疫刺激剂的组合导致适合于治疗诸如转移性癌症的免疫应答。