公开(公告)号：US08324570B2

公开(公告)日：2012-12-04

申请号：US13249716

申请日：2011-09-30

Applicant: Justin Wiseman ,  Nathalie Agar

Inventor： Justin Wiseman ,  Nathalie Agar

IPC: H01J49/04

CPC classification number: H01J49/0459 ,  G01N35/1095 ,  Y10T29/49826

Abstract: A system and sampling probe adaptable to an ultrasonic surgical instrument applies irrigation fluid and ultrasonic or vibrational energy to a target, and aspirates material desorbed from the target into a pick-up conduit. A suction source at the distal end of the conduit may aspirate the material released from the target with the irrigation fluid, thus efficiently sampling a broad range of materials from an arbitrary target to produce an analyzable effluent analyte stream which may be ionized and provided to the inlet of an ion-type analysis instrument, or may be fed directly to an instrument such as a flow cytometer, IR or fluorescence spectrophotometer, or other analyzer. Carrier gas may be provided to more effectively transport the desorbed material, and the probe may be incorporated into a robotic device to automatically carry out surface imaging or to effect sampling in hazardous environments.

Abstract translation: 适用于超声波手术器械的系统和采样探头将冲洗液和超声波或振动能量施加到目标，并将从靶标解吸的材料吸入吸气管。 导管远端的吸入源可以利用冲洗流体吸收从目标物释放的物质，从​​而有效地从任意目标采样各种材料，以产生可分析的流出物分析物流，其可被离子化并提供给 离子型分析仪器的入口，或者可以直接进入仪器，如流式细胞仪，红外或荧光分光光度计或其他分析仪。 可以提供载气以更有效地运输解吸的材料，并且探针可以结合到机器人装置中以自动进行表面成像或在危险环境中进行取样。

公开(公告)号：US20050230635A1

公开(公告)日：2005-10-20

申请号：US11090455

申请日：2005-03-25

Applicant: Zoltan Takats ,  Bogdan Gologan ,  Justin Wiseman ,  Robert Cooks

Inventor： Zoltan Takats ,  Bogdan Gologan ,  Justin Wiseman ,  Robert Cooks

IPC: H01J27/00 ,  H01J49/04

CPC classification number: H01J49/142 ,  H01J49/0004 ,  H01J49/0404

Abstract: A new method and system for desorption ionization is described and applied to the ionization of various compounds, including peptides and proteins present on metal, polymer, and mineral surfaces. Desorption electrospray ionization (DESI) is carried out by directing charged droplets and/or ions of a liquid onto the surface to be analyzed. The impact of the charged particles on the surface produces gaseous ions of material originally present on the surface. The resulting mass spectra are similar to normal ESI mass spectra in that they show mainly singly or multiply charged molecular ions of the analytes. The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins. Changes in the solution that is sprayed can be used to selectively ionize particular compounds, including those in biological matrices. In vivo analysis is demonstrated.

Abstract translation: 一种解吸电离的新方法和系统被描述和应用于各种化合物的电离，包括存在于金属，聚合物和矿物表面上的肽和蛋白质。 通过将液体的带电液滴和/或离子引导到要分析的表面上来进行解吸电喷雾离子化（DESI）。 带电粒子对表面的影响产生了最初存在于表面上的材料的气态离子。 所得质谱与正常的ESI质谱相似，因为它们主要表现出分析物的单一或多重带电分子离子。 在导电性和绝缘体表面以及通过极性化合物如肽和蛋白质从非极性小分子（如番茄红素，生物碱美西汀和小药物）的化合物观察到DESI现象。 喷雾溶液中的变化可用于选择性地电离特定化合物，包括生物基质中的化合物。 证明体内分析。

公开(公告)号：US20120080592A1

公开(公告)日：2012-04-05

申请号：US13249716

申请日：2011-09-30

Applicant: Justin Wiseman ,  Nathalie Agar

Inventor： Justin Wiseman ,  Nathalie Agar

IPC: H01J49/26 ,  H01J9/00 ,  H01J49/40

CPC classification number: H01J49/0459 ,  G01N35/1095 ,  Y10T29/49826

Abstract: A system and sampling probe adaptable to an ultrasonic surgical instrument applies irrigation fluid and ultrasonic or vibrational energy to a target, and aspirates material desorbed from the target into a pick-up conduit. A suction source at the distal end of the conduit may aspirate the material released from the target with the irrigation fluid, thus efficiently sampling a broad range of materials from an arbitrary target to produce an analyzable effluent analyte stream which may be ionized and provided to the inlet of an ion-type analysis instrument, or may be fed directly to an instrument such as a flow cytometer, IR or fluorescence spectrophotometer, or other analyzer. Carrier gas may be provided to more effectively transport the desorbed material, and the probe may be incorporated into a robotic device to automatically carry out surface imaging or to effect sampling in hazardous environments.

Abstract translation: 适用于超声波手术器械的系统和采样探头将冲洗液和超声波或振动能量施加到目标，并将从靶标解吸的材料吸入吸气管。 导管远端的吸入源可以利用冲洗流体吸收从目标物释放的物质，从​​而有效地从任意目标采样各种材料，以产生可分析的流出物分析物流，其可被离子化并提供给 离子型分析仪器的入口，或者可以直接进入仪器，如流式细胞仪，红外或荧光分光光度计或其他分析仪。 可以提供载气以更有效地运输解吸的材料，并且探针可以结合到机器人装置中以自动进行表面成像或在危险环境中进行取样。

公开(公告)号：US20070187589A1

公开(公告)日：2007-08-16

申请号：US11653569

申请日：2007-01-16

Applicant: Robert Cooks ,  Bogdan Gologan ,  Zoltan Takats ,  Justin Wiseman ,  Ismael Cotte-Rodriguez

Inventor： Robert Cooks ,  Bogdan Gologan ,  Zoltan Takats ,  Justin Wiseman ,  Ismael Cotte-Rodriguez

IPC: H01J49/00

CPC classification number: H01J49/14

Abstract: A desorption atmospheric pressure chemical ionization (DAPCI) system delivers a primary ion beam composed of an inert, high velocity gas and solvent ions to a surface to effect desorption and ionization of both volatile and non-volatile species present on surfaces. A electrode having a tapered tip is connected to a high voltage power supply. The tapered tip projects outward from a capillary carrying a high-speed flow of gas. A vapor of a solvent is mixed into the annular gas flow surrounding the needle. The gaseous solvent vapor is ionized in close proximity to the tapered tip by virtue of the high voltage applied to the electrode. The high-speed flow of gas and solvent vapor ions extending outward from the capillary is directed toward a substrate on which an analyte of interest may have been deposited. The solvent vapor ions can blanket the surface of the analyte causing a static charge build up that facilitates ion desorption and additionally can provide positive ion adducts of the analyte freed from the substrate surface that can be directed toward an atmospheric intake of a mass spectrometer or other instrument capable of studying the analyte.

Abstract translation: 解吸大气压化学电离（DAPCI）系统将由惰性高速气体和溶剂离子组成的初级离子束传送到表面，以实现表面上存在的挥发性和非挥发性物质的解吸和离子化。 具有锥形尖端的电极连接到高压电源。 锥形尖端从携带高速气体流的毛细管向外突出。 将溶剂的蒸气混合到围绕针的环形气流中。 通过施加到电极的高电压，气态溶剂蒸气被离子化，紧邻锥形尖端。 从毛细管向外延伸的气体和溶剂蒸汽离子的高速流动被引导到其上可能沉积感兴趣的分析物的基底。 溶剂蒸汽离子可以覆盖分析物的表面，导致静电荷积聚，促进离子解吸，并且另外可以提供从衬底表面释放的分析物的阳离子加合物，其可以被引导到大气摄入的质谱仪或其它 能够研究分析物的仪器。