公开(公告)号：US20100205754A1

公开(公告)日：2010-08-19

申请号：US12084287

申请日：2006-04-14

申请人： Kaori Otsuka ,  Yoshio Tsujino ,  Yusuke Konno ,  Shinji Kurose ,  Kunishige Kataoka ,  Takeshi Sakurai

发明人： Kaori Otsuka ,  Yoshio Tsujino ,  Yusuke Konno ,  Shinji Kurose ,  Kunishige Kataoka ,  Takeshi Sakurai

IPC分类号： C12N9/02 ,  C12N15/11 ,  C12N15/70 ,  C12P21/02 ,  C12N1/20 ,  D06P3/08

CPC分类号： A61Q5/10 ,  A61K8/66 ,  C12N9/0004

摘要： Modified CueO having excellent enzymatic activity and a composition for dyeing keratin fiber which contains the enzyme. A recombinant protein having an enzymatic activity for oxidizing p-phenylenediamine, the protein is obtained by removing from CueO at least one member selected from the group consisting of helix 5, helix 6, and helix 7; and the composition for dyeing keratin fiber containing the recombinant protein and an oxidation dye.

摘要翻译： 具有优异酶活性的改良CueO和含有酶的角蛋白纤维染色组合物。 具有氧化对苯二胺的酶活性的重组蛋白质，该蛋白质通过从CueO中除去选自螺旋5，螺旋6和螺旋7的至少一种而获得; 以及用于染色含有重组蛋白和氧化染料的角蛋白纤维的组合物。

公开(公告)号：US20060021152A1

公开(公告)日：2006-02-02

申请号：US10540156

申请日：2004-03-04

申请人： Yoshio Tsujino ,  Katsunori Endo ,  Abul Khaer Mohamad Quamrul Hasan ,  Kaori Otsuka

发明人： Yoshio Tsujino ,  Katsunori Endo ,  Abul Khaer Mohamad Quamrul Hasan ,  Kaori Otsuka

IPC分类号： D21D1/40

CPC分类号： A61Q5/10 ,  A61K8/66 ,  C12N9/001 ,  D06P1/32 ,  D06P3/08

摘要： The present invention relates to a neutral phenol oxidase showing small changes depending on a substrate in optimum pH for various substrates and having high enzymatic activity at pH around neutral region, a method for producing the same, and an antibody raised against the neutral phenol oxidase. According to the present invention, dyeing of fibers, coloring of hair or the like can be achieved. In addition, according to the present invention, effects on the environment, the human body, and the like can be reduced.

摘要翻译： 本发明涉及一种中性苯酚氧化酶，其在各种底物的最适pH下基于底物显示出小的变化，并且在中性部分附近的pH附近具有高的酶活性，其制备方法和针对中性酚氧化酶产生的抗体。 根据本发明，可以实现纤维染色，毛发着色等。 另外，根据本发明，能够降低对环境，人体等的影响。

公开(公告)号：US07939307B2

公开(公告)日：2011-05-10

申请号：US12084287

申请日：2006-04-14

申请人： Kaori Otsuka ,  Yoshio Tsujino ,  Yusuke Konno ,  Shinji Kurose ,  Kunishige Kataoka ,  Takeshi Sakurai

发明人： Kaori Otsuka ,  Yoshio Tsujino ,  Yusuke Konno ,  Shinji Kurose ,  Kunishige Kataoka ,  Takeshi Sakurai

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C09B67/00

CPC分类号： A61Q5/10 ,  A61K8/66 ,  C12N9/0004

摘要： Modified CueO having excellent enzymatic activity and a composition for dyeing keratin fiber which contains the enzyme. A recombinant protein having an enzymatic activity for oxidizing p-phenylenediamine, the protein is obtained by removing from CueO at least one member selected from the group consisting of helix 5, helix 6, and helix 7; and the composition for dyeing keratin fiber containing the recombinant protein and an oxidation dye.

摘要翻译： 具有优异酶活性的改良CueO和含有酶的角蛋白纤维染色组合物。 具有氧化对苯二胺的酶活性的重组蛋白质，该蛋白质通过从CueO中除去选自螺旋5，螺旋6和螺旋7的至少一种而获得; 以及用于染色含有重组蛋白和氧化染料的角蛋白纤维的组合物。