公开(公告)号：US07816511B2

公开(公告)日：2010-10-19

申请号：US10383241

申请日：2003-07-16

申请人： Kayoko Kawashima ,  Naruto Katsuragi ,  Keijiro Sugimura ,  Mayumi Furuya ,  Ryuichi Morishita

发明人： Kayoko Kawashima ,  Naruto Katsuragi ,  Keijiro Sugimura ,  Mayumi Furuya ,  Ryuichi Morishita

IPC分类号： C07H21/04 ,  A01N43/04 ,  A61K31/70

CPC分类号： C07K14/475 ,  A01K2217/05 ,  A61K39/00 ,  C07K14/47

摘要： The present invention provides methods for screening drugs inhibiting the expression of OSF-2 gene or the production or function of the protein encoded thereby and therapeutic agents for heart failure having such effects. Useful methods for diagnosing heart failure can be provided by monitoring the expression or variation of said gene or the production of the protein encoded thereby. The present invention also provides transgenic animals with forced expression of OSF-2 gene and methods for studying changes in gene expression or protein production or the functions of various genes or proteins with the progress of the pathology of heart failure using them and novel therapeutic agents for heart failure.

摘要翻译： 本发明提供了筛选抑制OSF-2基因表达的药物的方法或由其编码的蛋白质的产生或功能以及具有这种作用的心力衰竭治疗剂。 可以通过监测所述基因的表达或变异或由其编码的蛋白质的产生来提供用于诊断心力衰竭的有用方法。 本发明还提供了强力表达OSF-2基因的转基因动物以及用于研究基因表达或蛋白质生产的变化的方法，或者使用它们的心力衰竭病理进展的各种基因或蛋白质的功能和新的治疗剂 心脏衰竭。

公开(公告)号：US20090325288A1

公开(公告)日：2009-12-31

申请号：US12298565

申请日：2007-04-27

申请人： Uichi Koshimizu ,  Tomofumi Tanaka ,  Kayoko Kawashima ,  Michinori Kadokura

发明人： Uichi Koshimizu ,  Tomofumi Tanaka ,  Kayoko Kawashima ,  Michinori Kadokura

IPC分类号： C12N5/08 ,  C12N5/02

CPC分类号： C12N5/0657 ,  C12N2501/415 ,  C12N2506/02

摘要： The present invention provides a method for inducing differentiation of cardiomyocytes efficiently and selectively from stem cells.A method for inducing differentiation of cardiomyocytes from pluripotent stem cells, which comprises: (i) culturing the pluripotent stem cells in a culture medium containing no substance that promotes activation of the canonical Wnt signaling pathway during the time period between initiation of differentiation induction and 24 hours before the period of elevated canonical Wnt gene expression; and then (ii) culturing the pluripotent stem cells in a culture medium containing a substance that promotes activation of the canonical Wnt signaling pathway during a time period of 24 to 96 hours, starting from 24 to 0 hours before the period of elevated canonical Wnt gene expression.

摘要翻译： 本发明提供了从干细胞中有效且选择性地诱导心肌细胞分化的方法。 一种从多能干细胞诱导心肌细胞分化的方法，其特征在于：（i）在分化诱导开始和诱导分化诱导期间的时间段内培养不含有促进典型Wnt信号通路活化的物质的培养基中的多能干细胞 在正常Wnt基因表达升高之前的几个小时; 然后（ii）在培养基中培养多能干细胞，培养基含有促进典型Wnt信号通路活化的物质的培养基，时间为24至96小时，从高达标准Wnt基因的时间之前的24至0小时开始 表达。

公开(公告)号：US08293529B2

公开(公告)日：2012-10-23

申请号：US12298565

申请日：2007-04-27

申请人： Uichi Koshimizu ,  Tomofumi Tanaka ,  Kayoko Kawashima ,  Michinori Kadokura

发明人： Uichi Koshimizu ,  Tomofumi Tanaka ,  Kayoko Kawashima ,  Michinori Kadokura

IPC分类号： C12N5/00

CPC分类号： C12N5/0657 ,  C12N2501/415 ,  C12N2506/02

摘要： The present invention provides a method for inducing differentiation of cardiomyocytes efficiently and selectively from stem cells.A method for inducing differentiation of cardiomyocytes from pluripotent stem cells, which comprises: (i) culturing the pluripotent stem cells in a culture medium containing no substance that promotes activation of the canonical Wnt signaling pathway during the time period between initiation of differentiation induction and 24 hours before the period of elevated canonical Wnt gene expression; and then (ii) culturing the pluripotent stem cells in a culture medium containing a substance that promotes activation of the canonical Wnt signaling pathway during a time period of 24 to 96 hours, starting from 24 to 0 hours before the period of elevated canonical Wnt gene expression.

摘要翻译： 本发明提供了从干细胞中有效且选择性地诱导心肌细胞分化的方法。 一种从多能干细胞诱导心肌细胞分化的方法，其特征在于：（i）在分化诱导开始和诱导分化诱导期间的时间段内培养不含有促进典型Wnt信号通路活化的物质的培养基中的多能干细胞 在正常Wnt基因表达升高之前的几个小时; 然后（ii）在培养基中培养多能干细胞，培养基含有促进典型Wnt信号通路活化的物质的培养基，时间为24至96小时，从高达标准Wnt基因的时间之前的24至0小时开始 表达。