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公开(公告)号：US5840550A
公开(公告)日：1998-11-24
申请号：US445533
申请日：1995-05-22
CPC分类号： C12N9/1051 , C12N15/8241 , C12N15/8261 , C12Y204/01207
摘要： Endo-xyloglucanase transferases responsible for growth of plant cell wall, genes coding for the enzymes, a method of transferring xyloglucan molecules by using the enzyme, and methods of using the gene are described.
摘要翻译： 描述了负责植物细胞壁生长的内切葡聚糖酶转移酶，编码酶的基因，使用酶转移木葡聚糖分子的方法，以及使用该基因的方法。
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公开(公告)号：US6120998A
公开(公告)日：2000-09-19
申请号：US52085
申请日：1998-03-31
CPC分类号： C12N9/1051 , C12N15/8241 , C12N15/8261 , C12Y204/01207
摘要： Endo-xyloglucan transferases responsible for growth of plant cell wall, genes coding for the enzymes, a method of transferring xyloglucan molecules by using the enzyme, and methods of using the gene are described.
摘要翻译： 描述了负责植物细胞壁生长的内葡聚糖转移酶，编码酶的基因，使用酶转移木葡聚糖分子的方法，以及使用该基因的方法。
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公开(公告)号：US5516694A
公开(公告)日：1996-05-14
申请号：US381280
申请日：1995-01-31
CPC分类号： C12N9/1051 , C12N15/8241 , C12N15/8261 , C12Y204/01207
摘要： Endo-xyloglucan transferases responsible for growth of plant cell wall, genes coding for the enzymes, a method of transferring xyloglucan molecules by using the enzyme, and methods of using the gene are described.
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4.发明授权Feraxanase, a highly specific enzyme for hydrolysis of complex polysaccharides 失效拟旺曲酶，一种高度特异性的水解复合多糖的酶
公开(公告)号：US4954447A
公开(公告)日：1990-09-04
申请号：US223125
申请日：1988-07-22
CPC分类号： C12Y302/01136 , C12N9/248 , Y10S435/832 , Y10S435/839
摘要： This invention relates to a composition comprising a substantially pure enzyme capable of degrading arabinoxylan and having a molecular weight of about 45,000. More preferably this invention relates to such an enzyme which has a pH optimum between about 6.5 to about 7.0. The enzyme is derived from bacteria capable of hydroloyzing plant cell wall material, preferably from Bacillus most preferably from Bacillus subtilis. This invention further relates to such a composition which is further capable of selectively dissociating feraxan from a maize cell wall preparation. Additionally, compositions of this invention are unable to degrade Rhodymenia (1.fwdarw.3),(1.fwdarw.4)-.beta.-D-xylan and larch arabino-(1.fwdarw.4)-.beta.-D-xylan. Compositions of the present invention are capable of releasing fragments in a maize cell wall preparation at the glycosidic linkages of C1 and C5 arabinofuranosyl; C1 of terminal arabinofuranosyl; C2 and C4 of xylopyranosyl; C3 and C4 of xylopyranosyl, C1 and C3 of xylopyranosyl and C1 of terminal glucuronosyl. Compositions contemplated by the invention are also capable of releasing 2,4/3,4-linked-xylopyranosyl, terminal-arabinofuranosyl, 5-linked-arabinofuranosyl, 4-linked-xylopyranosyl, terminal-glucurono-pyranosyl and esterified ferulic acid from maize arabinoxylan. These compositions are preferably prepared from crude Bacillus sp. extracts by using a purification step selected from at least one of the following steps: differential precipitation using salts, ionic exchange chromatography, molecular filtration, HPLC, ultrafiltration and diafiltration.
摘要翻译： 本发明涉及包含能够降解阿拉伯木聚糖并具有约45,000分子量的基本上纯的酶的组合物。 更优选地，本发明涉及这样的酶，其pH最优值在约6.5至约7.0之间。 该酶源自能够使植物细胞壁材料加氢合成的细菌，优选来自芽孢杆菌，最优选来自枯草芽孢杆菌。 本发明进一步涉及这样的组合物，其还能够选择性地从玉米细胞壁制备物中分解法兰克生。 此外，本发明的组合物不能降解罗红膜菌素（1-> 3），（1-> 4）-β-D-木聚糖和落叶松阿拉伯糖 - （1-> 4）-β-D-木聚糖。 本发明的组合物能够在C1和C5阿糖呋喃糖苷的糖苷键上释放玉米细胞壁制剂中的片段; 末端阿拉伯呋喃糖基的C1; 吡喃木糖基的C2和C4; 木糖酰基的C3和C4，木糖基的C1和C3以及末端葡萄糖醛酸的C1。 本发明考虑的组合物还能够从玉米阿拉伯木聚糖中释放2,4 / 3,4-连接的木糖基，末端 - 阿拉伯呋喃糖基，5-连接的阿拉伯呋喃糖基，4-连接的 - 木糖基，末端 - 葡糖醛酸 - 吡喃糖基和酯化的阿魏酸 。 这些组合物优选由粗芽孢杆菌 通过使用选自以下步骤中的至少一个的纯化步骤：使用盐进行差示沉淀，离子交换层析，分子过滤，HPLC，超滤和渗滤。
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