公开(公告)号：US20100190159A1

公开(公告)日：2010-07-29

申请号：US12520115

申请日：2007-12-19

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07D493/10 ,  C07D311/78 ,  C12Q1/02 ,  G01N33/00

CPC分类号： G01N33/533 ,  C07D493/10 ,  C09B11/24 ,  G01N33/582 ,  Y02P20/55 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound used in gene analyses, which is highly stable and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a compound represented by the following formula (1) or (2): wherein R1 represents an alkyl group containing 1 to 6 carbon atoms, an amino group, an amide group optionally having a protecting group or a substituent, a halogen atom, or a hydrogen atom; R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom; R3 represents an alkyl group containing 1 to 6 carbon atoms, an aryl group, or a hydrogen atom; R4 represents a group containing an oxygen atom, or a hydrogen atom, wherein R3 may bind to R4 to form a ring.

摘要翻译： 本发明的目的是提供一种高度稳定且高度敏感的基因分析中使用的开关型荧光化合物，能够扩增微量的基因信号并进行观察。 本发明提供由下式（1）或（2）表示的化合物：其中R 1表示含有1至6个碳原子的烷基，氨基，任选具有保护基或取代基的酰胺基，卤素 原子或氢原子; R2独立地表示含有1至6个碳原子的烷基，卤素原子或氢原子; R3表示含有1至6个碳原子的烷基，芳基或氢原子; R4表示含有氧原子或氢原子的基团，其中R3可以与R4结合形成环。

公开(公告)号：US20110076680A1

公开(公告)日：2011-03-31

申请号：US12677014

申请日：2008-08-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07D311/78 ,  G01N33/50

CPC分类号： C07D493/10 ,  C09B11/08 ,  G01N33/533 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound (a fluorescence-producing molecule system) used in gene analyses, which is highly stable (namely, being active for a long period of time) and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a nonfluorescent molecule having a fluorescent substance skeleton such as fluorescein skeleton and having a group represented by —O—C(Y1)(Y2)-N3 wherein each of Y1 and Y2 represents a hydrogen atom, an alkyl group containing 1 to 6 carbon atoms, an alkoxy group containing 1 to 6 carbon atoms, an aryl group containing 6 to 10 carbon atoms, or a cyano group, in the molecule.

摘要翻译： 本发明的目的是提供用于基因分析的开关型荧光化合物（荧光生成分子体系），其高度稳定（即长时间活性）和高度敏感， 并能够扩增微量的基因信号并进行观察。 本发明提供具有荧光素骨架的荧光体骨架的非荧光分子，具有由-O-C（Y1）（Y2）-N3表示的基团，其中Y1和Y2各自表示氢原子，含有1个 至6个碳原子，含有1至6个碳原子的烷氧基，含有6至10个碳原子的芳基或氰基。

公开(公告)号：US08426127B2

公开(公告)日：2013-04-23

申请号：US12520115

申请日：2007-12-19

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07D307/77

CPC分类号： G01N33/533 ,  C07D493/10 ,  C09B11/24 ,  G01N33/582 ,  Y02P20/55 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound used in gene analyses, which is highly stable and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a compound represented by the following formula (1) or (2): wherein R1 represents an alkyl group containing 1 to 6 carbon atoms, an amino group, an amide group optionally having a protecting group or a substituent, a halogen atom, or a hydrogen atom; R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom; R3 represents an alkyl group containing 1 to 6 carbon atoms, an aryl group, or a hydrogen atom; R4 represents a group containing an oxygen atom, or a hydrogen atom, wherein R3 may bind to R4 to form a ring.

摘要翻译： 本发明的目的是提供一种高度稳定且高度敏感的基因分析中使用的开关型荧光化合物，能够扩增微量的基因信号并进行观察。 本发明提供由下式（1）或（2）表示的化合物：其中R 1表示含有1至6个碳原子的烷基，氨基，任选具有保护基或取代基的酰胺基，卤素 原子或氢原子; R2独立地表示含有1至6个碳原子的烷基，卤素原子或氢原子; R3表示含有1至6个碳原子的烷基，芳基或氢原子; R4表示含有氧原子或氢原子的基团，其中R3可以与R4结合形成环。

公开(公告)号：US08084201B2

公开(公告)日：2011-12-27

申请号：US12677014

申请日：2008-08-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07D493/10 ,  C09B11/08 ,  G01N33/533 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound (a fluorescence-producing molecule system) used in gene analyses, which is highly stable (namely, being active for a long period of time) and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a nonfluorescent molecule having a fluorescent substance skeleton such as fluorescein skeleton and having a group represented by —O—C(Y1)(Y2)-N3 wherein each of Y1 and Y2 represents a hydrogen atom, an alkyl group containing 1 to 6 carbon atoms, an alkoxy group containing 1 to 6 carbon atoms, an aryl group containing 6 to 10 carbon atoms, or a cyano group, in the molecule.

摘要翻译： 本发明的目的是提供用于基因分析的开关型荧光化合物（荧光生成分子体系），其高度稳定（即长时间活性）和高度敏感， 并能够扩增微量的基因信号并进行观察。 本发明提供具有荧光素骨架的荧光体骨架的非荧光分子，具有由-O-C（Y1）（Y2）-N3表示的基团，其中Y1和Y2各自表示氢原子，含有1个 至6个碳原子，含有1至6个碳原子的烷氧基，含有6至10个碳原子的芳基或氰基。

公开(公告)号：US5275940A

公开(公告)日：1994-01-04

申请号：US748559

申请日：1991-08-22

申请人： Kuniki Kino ,  Kazuhiro Furukawa ,  Yasuhiro Tomiyoshi ,  Yoshiyuki Kuratsu

发明人： Kuniki Kino ,  Kazuhiro Furukawa ,  Yasuhiro Tomiyoshi ,  Yoshiyuki Kuratsu

IPC分类号： C12N1/21 ,  C12P13/22 ,  C12R1/15

CPC分类号： C12P13/227

摘要： Disclosed is a process for producing L-tryptophan which comprises culturing in a medium a microorganism belonging to the genus Corynebacterium or Brevibacterium having resistance to an aminoquinoline derivative or a phenothiazine derivative and an ability to produce L-tryptophan until L-tryptophan is accumulated in the culture and recovering L-tryptophan therefrom.

摘要翻译： 公开了一种生产L-色氨酸的方法，其包括在培养基中培养属于棒状杆菌属的微生物或对氨基喹啉衍生物或吩噻嗪衍生物具有抗性的微生物和产生L-色氨酸的能力，直到L-色氨酸积累在 培养并从中回收L-色氨酸。

公开(公告)号：US5459997A

公开(公告)日：1995-10-24

申请号：US215766

申请日：1994-03-22

申请人： Takashi Takemoto ,  Hideharu Iwakuni ,  Motoshi Kataoka ,  Kazuhiro Furukawa

发明人： Takashi Takemoto ,  Hideharu Iwakuni ,  Motoshi Kataoka ,  Kazuhiro Furukawa

IPC分类号： F01N3/22 ,  F01N3/24 ,  F01N11/00

CPC分类号： F01N3/222 ,  F01N11/00 ,  F01N3/22 ,  F01N2550/02 ,  Y02T10/47

摘要： An exhaust gas purification system having a catalyst for removing nitrogen oxides from exhaust gas causes the temperature range of activation of the catalyst to shift toward the higher temperature side when the degree of oxidation of the catalyst becomes pronounced.

摘要翻译： 具有从排气中除去氮氧化物的催化剂的排气净化系统使得当催化剂的氧化程度变得明显时，催化剂的活化温度范围向较高温度侧移动。