公开(公告)号：US20110076680A1

公开(公告)日：2011-03-31

申请号：US12677014

申请日：2008-08-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07D311/78 ,  G01N33/50

CPC分类号： C07D493/10 ,  C09B11/08 ,  G01N33/533 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound (a fluorescence-producing molecule system) used in gene analyses, which is highly stable (namely, being active for a long period of time) and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a nonfluorescent molecule having a fluorescent substance skeleton such as fluorescein skeleton and having a group represented by —O—C(Y1)(Y2)-N3 wherein each of Y1 and Y2 represents a hydrogen atom, an alkyl group containing 1 to 6 carbon atoms, an alkoxy group containing 1 to 6 carbon atoms, an aryl group containing 6 to 10 carbon atoms, or a cyano group, in the molecule.

摘要翻译： 本发明的目的是提供用于基因分析的开关型荧光化合物（荧光生成分子体系），其高度稳定（即长时间活性）和高度敏感， 并能够扩增微量的基因信号并进行观察。 本发明提供具有荧光素骨架的荧光体骨架的非荧光分子，具有由-O-C（Y1）（Y2）-N3表示的基团，其中Y1和Y2各自表示氢原子，含有1个 至6个碳原子，含有1至6个碳原子的烷氧基，含有6至10个碳原子的芳基或氰基。

公开(公告)号：US20100190159A1

公开(公告)日：2010-07-29

申请号：US12520115

申请日：2007-12-19

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07D493/10 ,  C07D311/78 ,  C12Q1/02 ,  G01N33/00

CPC分类号： G01N33/533 ,  C07D493/10 ,  C09B11/24 ,  G01N33/582 ,  Y02P20/55 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound used in gene analyses, which is highly stable and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a compound represented by the following formula (1) or (2): wherein R1 represents an alkyl group containing 1 to 6 carbon atoms, an amino group, an amide group optionally having a protecting group or a substituent, a halogen atom, or a hydrogen atom; R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom; R3 represents an alkyl group containing 1 to 6 carbon atoms, an aryl group, or a hydrogen atom; R4 represents a group containing an oxygen atom, or a hydrogen atom, wherein R3 may bind to R4 to form a ring.

摘要翻译： 本发明的目的是提供一种高度稳定且高度敏感的基因分析中使用的开关型荧光化合物，能够扩增微量的基因信号并进行观察。 本发明提供由下式（1）或（2）表示的化合物：其中R 1表示含有1至6个碳原子的烷基，氨基，任选具有保护基或取代基的酰胺基，卤素 原子或氢原子; R2独立地表示含有1至6个碳原子的烷基，卤素原子或氢原子; R3表示含有1至6个碳原子的烷基，芳基或氢原子; R4表示含有氧原子或氢原子的基团，其中R3可以与R4结合形成环。

公开(公告)号：US08426127B2

公开(公告)日：2013-04-23

申请号：US12520115

申请日：2007-12-19

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Jin Wang ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07D307/77

CPC分类号： G01N33/533 ,  C07D493/10 ,  C09B11/24 ,  G01N33/582 ,  Y02P20/55 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound used in gene analyses, which is highly stable and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a compound represented by the following formula (1) or (2): wherein R1 represents an alkyl group containing 1 to 6 carbon atoms, an amino group, an amide group optionally having a protecting group or a substituent, a halogen atom, or a hydrogen atom; R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom; R3 represents an alkyl group containing 1 to 6 carbon atoms, an aryl group, or a hydrogen atom; R4 represents a group containing an oxygen atom, or a hydrogen atom, wherein R3 may bind to R4 to form a ring.

摘要翻译： 本发明的目的是提供一种高度稳定且高度敏感的基因分析中使用的开关型荧光化合物，能够扩增微量的基因信号并进行观察。 本发明提供由下式（1）或（2）表示的化合物：其中R 1表示含有1至6个碳原子的烷基，氨基，任选具有保护基或取代基的酰胺基，卤素 原子或氢原子; R2独立地表示含有1至6个碳原子的烷基，卤素原子或氢原子; R3表示含有1至6个碳原子的烷基，芳基或氢原子; R4表示含有氧原子或氢原子的基团，其中R3可以与R4结合形成环。

公开(公告)号：US08084201B2

公开(公告)日：2011-12-27

申请号：US12677014

申请日：2008-08-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Kazuhiro Furukawa

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07D493/10 ,  C09B11/08 ,  G01N33/533 ,  Y10T436/143333

摘要： It is an object of the present invention to provide an on-off type fluorescent compound (a fluorescence-producing molecule system) used in gene analyses, which is highly stable (namely, being active for a long period of time) and highly sensitive, and which enables amplification of a trace amount of gene signal and observation thereof. The present invention provides a nonfluorescent molecule having a fluorescent substance skeleton such as fluorescein skeleton and having a group represented by —O—C(Y1)(Y2)-N3 wherein each of Y1 and Y2 represents a hydrogen atom, an alkyl group containing 1 to 6 carbon atoms, an alkoxy group containing 1 to 6 carbon atoms, an aryl group containing 6 to 10 carbon atoms, or a cyano group, in the molecule.

摘要翻译： 本发明的目的是提供用于基因分析的开关型荧光化合物（荧光生成分子体系），其高度稳定（即长时间活性）和高度敏感， 并能够扩增微量的基因信号并进行观察。 本发明提供具有荧光素骨架的荧光体骨架的非荧光分子，具有由-O-C（Y1）（Y2）-N3表示的基团，其中Y1和Y2各自表示氢原子，含有1个 至6个碳原子，含有1至6个碳原子的烷氧基，含有6至10个碳原子的芳基或氰基。

公开(公告)号：US08709721B2

公开(公告)日：2014-04-29

申请号：US13499826

申请日：2010-10-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： C12Q1/68 ,  C07D409/00 ,  C07H19/04

CPC分类号： G01N33/582 ,  C07D311/16 ,  C07D405/12 ,  C07H21/02 ,  C12Q1/6816 ,  C12Q1/6818 ,  G01N33/533 ,  Y02P20/55 ,  Y10T436/143333 ,  C12Q2523/107 ,  C12Q2563/107 ,  C12Q2565/101

摘要： Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I′): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.

摘要翻译： 提供了用于基因分析的荧光开/关可切换化合物，其高度稳定和高度灵敏，并且能够扩增和观察微量基因信号，以及用于检测生物相关材料的标记试剂，其使用荧光 开/关切换组合。 由下式（I'）表示的化合物：其中X是氢原子或羧酸保护基，Y是2,4-二硝基苯磺酰基，2-氰基-4-硝基苯磺酰基或5-硝基吡啶 -2-基磺酰基，R 1，R 2，R 3和R 4各自独立地为氢原子，卤素原子，羟基，碳数为1至6的烷基，碳数为1至 6，碳数为6〜10的芳基或氰基。

公开(公告)号：US20100137407A1

公开(公告)日：2010-06-03

申请号：US12598974

申请日：2008-05-09

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Naoko Abe ,  Hidekazu Toyobuku

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Naoko Abe ,  Hidekazu Toyobuku

IPC分类号： A61K31/7105 ,  C07H21/02 ,  C12P19/34 ,  C12N5/071 ,  A61P31/12

摘要： The present invention relates to a single-chain circular RNA having a sustained or slow-releasing RNA interference effect, characterized in that the single-chain circular RNA comprises a sense strand sequence, an antisense strand sequence complementary to the sense strand sequence, identical or different two loop sequences between the sense strand and the antisense strand, connecting both strands, wherein the sense strand and the antisense strand are paired to form a stem.

摘要翻译： 本发明涉及具有持续或缓慢释放的RNA干扰效应的单链环状RNA，其特征在于单链环状RNA包括有义链序列，与有义链序列互补的反义链序列，相同或 连接两条链之间的有义链和反义链之间的不同的两个环序列，其中有义链和反义链配对形成茎。

公开(公告)号：US20120196377A1

公开(公告)日：2012-08-02

申请号：US13499826

申请日：2010-10-01

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： G01N21/64 ,  C07D311/16

CPC分类号： G01N33/582 ,  C07D311/16 ,  C07D405/12 ,  C07H21/02 ,  C12Q1/6816 ,  C12Q1/6818 ,  G01N33/533 ,  Y02P20/55 ,  Y10T436/143333 ,  C12Q2523/107 ,  C12Q2563/107 ,  C12Q2565/101

摘要： Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I′): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.

摘要翻译： 提供了用于基因分析的荧光开/关可切换化合物，其高度稳定和高度灵敏，并且能够扩增和观察微量基因信号，以及用于检测生物相关材料的标记试剂，其使用荧光 开/关切换组合。 由下式（I'）表示的化合物：其中X是氢原子或羧酸保护基，Y是2,4-二硝基苯磺酰基，2-氰基-4-硝基苯磺酰基或5-硝基吡啶 -2-基磺酰基，R 1，R 2，R 3和R 4各自独立地为氢原子，卤素原子，羟基，碳数为1至6的烷基，碳数为1至 6，碳数为6〜10的芳基或氰基。

公开(公告)号：US08187825B2

公开(公告)日：2012-05-29

申请号：US12919299

申请日：2009-01-30

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： G01N33/574 ,  C12Q1/02 ,  A01N55/02 ,  A01N43/16 ,  A01N31/14

CPC分类号： C07D265/38 ,  C07D311/82 ,  C07D493/10 ,  G01N21/77 ,  G01N2021/7786 ,  Y10T436/16 ,  Y10T436/182

摘要： It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1): wherein each R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom.

摘要翻译： 本发明的目的是提供一种可用于体内的新型巯基检测试剂，其解决了水解产生背景荧光的问题; 和使用上述试剂检测硫醇的方法。 本发明提供由下式（1）表示的化合物：其中各R 2独立地表示含有1〜6个碳原子的烷基，卤素原子或氢原子。

公开(公告)号：US20110055965A1

公开(公告)日：2011-03-03

申请号：US12867090

申请日：2009-02-13

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Naoko Abe ,  Mitsuru Harada

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Naoko Abe ,  Mitsuru Harada

IPC分类号： A01H1/00 ,  C07H21/02 ,  C12N15/00 ,  A61K48/00

CPC分类号： C12N15/10 ,  A61K47/549 ,  A61K47/60 ,  C12N15/111 ,  C12N15/66 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2320/51 ,  C12N2330/30

摘要： The present invention relates to a circular single-stranded nucleic acid complex comprising a sense strand RNA sequence, an antisense strand RNA sequence complementary to the sense strand RNA sequence, and two identical or different loop moieties connecting the sense and antisense strands, wherein the sense and antisense strands form a stem by pairing, and the loop moieties are selected from the group consisting of polyalkylene glycol, DNA, DNA-RNA chimera, a derivative thereof, and a combination thereof.

摘要翻译： 本发明涉及包含有义链RNA序列，与有义链RNA序列互补的反义链RNA序列以及连接有义链和反义链的两个相同或不同的环部分的环状单链核酸复合物，其中该感觉 并且反义链通过配对形成茎，并且环部分选自聚亚烷基二醇，DNA，DNA-RNA嵌合体，其衍生物及其组合。

公开(公告)号：US20110111446A1

公开(公告)日：2011-05-12

申请号：US12919299

申请日：2009-01-30

申请人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

发明人： Hiroshi Abe ,  Yoshihiro Ito ,  Aya Shibata ,  Mika Ito

IPC分类号： C12Q1/02 ,  C07D265/34 ,  C07D493/10 ,  G01N33/00

CPC分类号： C07D265/38 ,  C07D311/82 ,  C07D493/10 ,  G01N21/77 ,  G01N2021/7786 ,  Y10T436/16 ,  Y10T436/182

摘要： It is an object of the present invention to provide: a novel thiol-detecting reagent, which can be used in vivo and which solves the problem regarding the generation of background fluorescence due to hydrolysis; and a method for detecting thiol using the aforementioned reagent. The present invention provides a compound represented by the following formula (1): wherein each R2 independently represents an alkyl group containing 1 to 6 carbon atoms, a halogen atom, or a hydrogen atom.

摘要翻译： 本发明的目的是提供一种可用于体内的新型巯基检测试剂，其解决了水解产生背景荧光的问题; 和使用上述试剂检测硫醇的方法。 本发明提供由下式（1）表示的化合物：其中各R 2独立地表示含有1〜6个碳原子的烷基，卤素原子或氢原子。