公开(公告)号：US20240043886A1

公开(公告)日：2024-02-08

申请号：US18464158

申请日：2023-09-08

申请人： CJ CHEILJEDANG CORPORATION

发明人： Han Hyoung LEE ,  Hyo Jeong BYUN ,  Byeong Soo KIM ,  Hee Ju KIM ,  Moo Young JUNG ,  Hyung Joon KIM ,  Seul-Gi PARK

IPC分类号： C12P13/22 ,  C12P13/08 ,  C12N9/04 ,  C12N9/12

CPC分类号： C12P13/227 ,  C12P13/08 ,  C12N9/0006 ,  C12Y101/01049 ,  C12N9/1205 ,  C12Y207/01004

摘要： The present disclosure relates to a microorganism of the genus Corynebacterium producing L-amino acid, a method for producing L-amino acid using the same, use of L-amino acid production, and a composition for producing L-amino acid.

公开(公告)号：US11879144B2

公开(公告)日：2024-01-23

申请号：US18152329

申请日：2023-01-10

申请人： University of Florida Research Foundation, Incorporated

发明人： Yousong Ding ,  Ran Zuo

IPC分类号： C12P13/22 ,  C12N9/04 ,  C12N9/02 ,  C12N15/62

CPC分类号： C12P13/227 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0075 ,  C12N15/62 ,  C12Y101/9901 ,  C12Y106/02004 ,  C12Y114/13039

摘要： The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.

公开(公告)号：US11879143B2

公开(公告)日：2024-01-23

申请号：US16691452

申请日：2019-11-21

申请人： California Institute of Technology

发明人： Andrew Richard Buller ,  Sabine Brinkmann-Chen ,  Michael Herger ,  David K. Romney ,  Javier Murciano Calles ,  Paul van Roye

IPC分类号： C12P13/22 ,  C12N9/88 ,  C12P13/04

CPC分类号： C12P13/227 ,  C12N9/88 ,  C12P13/04 ,  C12Y402/0102

摘要： This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids.

公开(公告)号：US20230287466A1

公开(公告)日：2023-09-14

申请号：US18152329

申请日：2023-01-10

申请人： UNIVERSITY OF FLORIDA RESEARCH

发明人： Yousong Ding ,  Ran Zuo

IPC分类号： C12P13/22 ,  C12N9/04 ,  C12N9/02 ,  C12N15/62

CPC分类号： C12P13/227 ,  C12N9/0006 ,  C12N9/0042 ,  C12N9/0075 ,  C12N15/62 ,  C12Y101/9901 ,  C12Y106/02004 ,  C12Y114/13039

摘要： The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.

公开(公告)号：US11746345B2

公开(公告)日：2023-09-05

申请号：US15165606

申请日：2016-05-26

申请人： AJINOMOTO CO., INC.

发明人： Valery Vasilievich Samsonov ,  Natalia Sergeevna Eremina ,  Natalia Viktorovna Stoynova

IPC分类号： C12N9/00 ,  C12P13/08 ,  C12P13/04 ,  C12N15/52 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/22 ,  C12P13/24

CPC分类号： C12N9/93 ,  C12N15/52 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/222 ,  C12P13/227 ,  C12P13/24 ,  C12Y603/02002

摘要： The present invention provides a method for producing an L-amino acid such as a branched-chain L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the gshA gene.

公开(公告)号：US09562246B2

公开(公告)日：2017-02-07

申请号：US14784621

申请日：2014-04-16

申请人： CJ Cheiljedang Corporation

发明人： Hyung Joon Kim ,  Ju No Jang ,  Kyung Rim Kim ,  Keun Cheol Lee ,  Young Bin Hwang ,  Hyeong Pyo Hong

IPC分类号： C12P13/22 ,  C12N9/10

CPC分类号： C12P13/227 ,  C12N9/1077 ,  C12Y204/02018 ,  Y02P20/52

摘要： The present invention relates to a microorganism of the genus Escherichia having enhanced L-tryptophan productivity, which has been modified to express yeast anthranilate phosphoribosyltransferase in order to be able to produce L-tryptophan at high concentration, and to a method for producing L-tryptophan, comprising a step of culturing the microorganism. The microorganism of the genus Escherichia can produce L-tryptophan, and thus can be advantageously used in the pharmaceutical industry and the feed industry, particularly for animal feed.

摘要翻译： 本发明涉及具有增强的L-色氨酸生产力的埃希氏菌属微生物，其已被修饰以表达酵母邻氨基苯甲酸酯磷酸核糖转移酶，以便能够产生高浓度的L-色氨酸，以及L-色氨酸的生产方法 包括培养微生物的步骤。 埃希氏菌属的微生物可产生L-色氨酸，因此可有利地用于制药工业和饲料工业，尤其用于动物饲料。

公开(公告)号：US09540652B2

公开(公告)日：2017-01-10

申请号：US14342335

申请日：2012-08-31

申请人： Darmawi Juminaga ,  Jay D. Keasling

发明人： Darmawi Juminaga ,  Jay D. Keasling

IPC分类号： C12N15/70 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P13/22 ,  C12N9/02

CPC分类号： C12N15/70 ,  C12N9/001 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P13/22 ,  C12P13/225 ,  C12P13/227 ,  C12Y103/01012 ,  C12Y401/02 ,  Y02P20/52

摘要： The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.

摘要翻译： 本公开涉及产生氨基酸和氨基酸中间体的工程微生物。 具体地，本公开涉及编码增加芳族氨基酸的产生和芳香族氨基酸中间体莽草酸的操纵子的重组核酸; 芳香族氨基酸生成微生物和芳香族氨基酸中间体莽草酸; 以及与芳香族氨基酸的生成，芳香族氨基酸中间体莽草酸以及从其衍生的商品化学品有关的方法。

公开(公告)号：US20160017317A1

公开(公告)日：2016-01-21

申请号：US14775025

申请日：2014-02-26

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： George M. Church ,  Srivatsan Raman ,  Noah D. Taylor ,  Jameson Rogers

IPC分类号： C12N15/10

CPC分类号： C12N15/1058 ,  C12N1/38 ,  C12N15/1034 ,  C12N15/52 ,  C12P1/04 ,  C12P5/02 ,  C12P7/02 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/58 ,  C12P13/04 ,  C12P13/14 ,  C12P13/227 ,  C12P17/06 ,  C12P17/08 ,  C12P17/18 ,  C12P17/188 ,  C12P19/02 ,  C12P19/12 ,  C12P19/42 ,  C12P19/44 ,  C12P29/00 ,  C12P33/00

摘要： The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.

摘要翻译： 本发明涉及转基因细菌和优化用于生产代谢物的转基因细菌的方法。

公开(公告)号：US20150044734A1

公开(公告)日：2015-02-12

申请号：US14342335

申请日：2012-08-31

申请人： Darmawi Juminaga ,  Jay D. Keasling

发明人： Darmawi Juminaga ,  Jay D. Keasling

IPC分类号： C12N15/70 ,  C12P13/22 ,  C12P7/42

CPC分类号： C12N15/70 ,  C12N9/001 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P13/22 ,  C12P13/225 ,  C12P13/227 ,  C12Y103/01012 ,  C12Y401/02 ,  Y02P20/52

摘要： The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.

摘要翻译： 本公开涉及产生氨基酸和氨基酸中间体的工程微生物。 具体地，本公开涉及编码增加芳族氨基酸的产生和芳香族氨基酸中间体莽草酸的操纵子的重组核酸; 芳香族氨基酸生成微生物和芳香族氨基酸中间体莽草酸; 以及与芳香族氨基酸的生成，芳香族氨基酸中间体莽草酸以及从其衍生的商品化学品有关的方法。

公开(公告)号：US08932834B2

公开(公告)日：2015-01-13

申请号：US13404148

申请日：2012-02-24

申请人： Hidetaka Doi ,  Yoshihiro Usuda

发明人： Hidetaka Doi ,  Yoshihiro Usuda

IPC分类号： C12P13/08 ,  C12P13/12 ,  C12P13/22 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N9/78 ,  C12N9/80

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/78 ,  C12N9/80 ,  C12P13/12 ,  C12P13/227 ,  C12Y203/01109 ,  C12Y206/01081 ,  C12Y305/01096 ,  C12Y305/03023

摘要： A method for producing an L-amino acid which includes the steps of culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid producing ability in a medium to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the medium, wherein the bacterium has been modified so that an activity or activities of one or two or more enzymes of the arginine succinyltransferase pathway, such as arginine succinyltransferase, succinylarginine dihydrolase, succinylornithine aminotransferase, succinylglutamate-semialdehyde dehydrogenase, and succinylglutamate desuccinylase, is/are decreased.

摘要翻译： 一种L-氨基酸的制造方法，其特征在于，在培养基中培养属于肠杆菌科并具有L-氨基酸生产能力的细菌的步骤，在培养基中生成并积累L-氨基酸，并收集 来自培养基的L-氨基酸，其中所述细菌已被修饰，使得精氨酸琥珀酰转移酶途径的一种或两种或多种酶的活性或活性，例如精氨酸琥珀酰转移酶，琥珀酰精氨酸二水解酶，琥珀酰鸟氨酸氨基转移酶，琥珀酰谷氨酸 - 半醛脱氢酶和 琥珀酰谷氨酸脱琥珀酰基酶降低。