公开(公告)号：US06806958B2

公开(公告)日：2004-10-19

申请号：US10262067

申请日：2002-10-02

申请人： Hiroaki Kojima ,  Kazuhiro Oiwa ,  Brenner Bernhard

发明人： Hiroaki Kojima ,  Kazuhiro Oiwa ,  Brenner Bernhard

IPC分类号： G01B902

CPC分类号： G01Q10/065 ,  G01Q20/02 ,  G01Q60/42

摘要： A method and an apparatus for measuring the microforce acting between a micro particle fixed on the probe and the observation plane are described. The probe is feedback-controlled to be held motionless by canceling probe displacements caused by movements of the micro particle fixed on the probe, using irradiation pressure provided by a laser that applies photon pressure. The microforce acting between the micro particle fixed on the probe and the observation plane is measured by recording the time-varying laser output power. This allows probe fluctuations to be reduced to a few angstroms, and permits control of the distance to the target object to within a few nm, enabling measurement of microforces on the order of 0.1 pN.

摘要翻译： 描述了用于测量在固定在探针上的微粒子与观察平面之间作用的微力的方法和装置。 通过使用由施加光子压力的激光器提供的照射压力，通过消除由固定在探针上的微粒子的移动引起的探针位移，探测器被反馈控制成不动。 通过记录时变激光输出功率来测量作用在固定在探针上的微粒子与观察平面之间的微小力。 这允许探针波动降低到几埃，并且允许将目标物体的距离控制在几nm内，使得能够测量约0.1pN的微力。

公开(公告)号：US07939289B2

公开(公告)日：2011-05-10

申请号：US11186026

申请日：2005-07-21

申请人： Akira Yamada ,  Kazuhiro Oiwa

发明人： Akira Yamada ,  Kazuhiro Oiwa

IPC分类号： C12Q1/25 ,  C07K14/00

CPC分类号： G01N33/6887

摘要： A distinction method is provided to distinguish catch capacity where myosin, a protein that constitutes muscles, or filaments containing myosin bind to actin due to the contribution of twitchin that constitutes thick filaments along with myosin, while certain tension is sustained. The catch capacity is distinguished by using synthetic thick filaments containing myosin and twitchin, which are obtained from an extract of the muscles with a predetermined high salt buffer solution, and a soluble protein fraction obtained from the suspension of the above-mentioned muscles.

摘要翻译： 提供了一种区分捕获能力，其中肌球蛋白（构成肌肉的蛋白质）或含有肌球蛋白的细丝与肌动蛋白结合，因为在维持某些张力的同时，构成细长丝的肌肉蛋白与肌球蛋白一起的贡献。 通过使用由预定的高盐缓冲溶液的肌肉提取物获得的含有肌球蛋白和twitchin的合成粗细丝以及由上述肌肉的悬浮液获得的可溶性蛋白质级分来区分捕获能力。

公开(公告)号：US20060105463A1

公开(公告)日：2006-05-18

申请号：US11186026

申请日：2005-07-21

申请人： Akira Yamada ,  Kazuhiro Oiwa

发明人： Akira Yamada ,  Kazuhiro Oiwa

IPC分类号： G01N33/48

CPC分类号： G01N33/6887

摘要： A distinction method is provided to distinguish catch capacity where myosin, a protein that constitutes muscles, or filaments containing myosin bind to actin due to the contribution of twitchin that constitutes thick filaments along with myosin, while certain tension is sustained. The catch capacity is distinguished by using synthetic thick filaments containing myosin and twitchin, which are obtained from an extract of the muscles with a predetermined high salt buffer solution, and a soluble protein fraction obtained from the suspension of the above-mentioned muscles.

摘要翻译： 提供了一种区分捕获能力，其中肌球蛋白（构成肌肉的蛋白质）或含有肌球蛋白的细丝与肌动蛋白结合，因为在维持某些张力的同时，构成细长丝的肌肉蛋白与肌球蛋白一起的贡献。 通过使用由预定的高盐缓冲溶液的肌肉提取物获得的含有肌球蛋白和twitchin的合成粗细丝以及由上述肌肉的悬浮液获得的可溶性蛋白质级分来区分捕获能力。

公开(公告)号：US20060104967A1

公开(公告)日：2006-05-18

申请号：US11186023

申请日：2005-07-21

申请人： Akira Yamada ,  Kazuhiro Oiwa

发明人： Akira Yamada ,  Kazuhiro Oiwa

IPC分类号： A61K38/46

CPC分类号： A61K38/1738 ,  A61K38/45 ,  A61K38/465 ,  C12Y207/11011 ,  C12Y301/03016 ,  A61K2300/00

摘要： Since the mechanism of conversion of the state of actin and myosin or the filaments containing myosin from the relaxed to the catch had not been totally clear and its reconstitution was not easily carried out, a method of converting the state of the muscle proteins from the relaxed state, where said actin does not bind to myosin or filaments containing myosin, to the catch state, where actin binds to myosin or the filaments containing myosin and the binding can sustain a certain tension with low energy expenditure, is accomplished by using a phosphatase called serine/threonine protein phosphatase 2B dephosphorylating twitchin. The phosphatase forms thick filaments along with myosin, and at the same time, the state is converted from the catch state to the relaxed state by using a protein kinase A phosphorylating twitchin, and thus the state can be reversibly converted between the catch and the relaxed states.

摘要翻译： 由于将肌动蛋白和肌球蛋白或含有肌球蛋白的细丝从松弛状态转变为捕获物的机制尚不完全清楚，并且其重构不容易进行，因此将肌肉蛋白质的状态从放松状态 状态，其中所述肌动蛋白不结合含有肌球蛋白的肌球蛋白或细丝到捕获状态，其中肌动蛋白结合肌球蛋白或含有肌球蛋白的丝和结合可以以低能量消耗维持一定的张力，通过使用称为 丝氨酸/苏氨酸蛋白磷酸酶2B去磷酸化twitchin。 磷酸酶与肌球蛋白一起形成细长丝，同时通过使用蛋白激酶A磷酸化twitchin将状态从捕获状态转变为松弛状态，因此状态可以在捕获和放松之间可逆地转化 状态。