公开(公告)号：US08741583B2

公开(公告)日：2014-06-03

申请号：US13379459

申请日：2010-03-31

申请人： Kiyotaka Toshimori ,  Chizuru Ito ,  Kenji Yamatoya ,  Keiichi Yoshida

发明人： Kiyotaka Toshimori ,  Chizuru Ito ,  Kenji Yamatoya ,  Keiichi Yoshida

IPC分类号： G01N33/53

CPC分类号： G01N33/5044 ,  G01N33/689 ,  G01N2500/00

摘要： A method of identifying compounds that inhibit fertilization is provided. The method can include selecting compounds that bind to equatorin protein. Two types of equatorin protein, a long form and a short form, can be present in the testis. The amino acid sequence of mouse equatorin from positions 101 to 146 including the 138th O-glycosylated threonine residue contains an epitope recognized by anti-equatorin antibody MN9 that has an effect of inhibiting fertilization. In addition, the MN9 antibody also binds to human sperm. Compounds that bind to the epitope can inhibit fertilization. Both forms of mouse equatorin can be used as well as human equatorin to identify compounds that inhibit fertilization.

摘要翻译： 提供了鉴定抑制受精的化合物的方法。 该方法可以包括选择结合赤铁蛋白的化合物。 睾丸中可以存在两种类型的赤道蛋白，长形式和短型。 含有第138位O-糖基化苏氨酸残基的101〜146位的小鼠赤尾蛋白的氨基酸序列含有抗赤道素抗体MN9所识别的具有抑制受精作用的表位。 此外，MN9抗体还结合人精子。 与表位结合的化合物可以抑制受精。 可以使用两种形式的小鼠赤尾蛋白以及人类赤道蛋白来鉴定抑制受精的化合物。

公开(公告)号：US20120122244A1

公开(公告)日：2012-05-17

申请号：US13379459

申请日：2010-03-31

申请人： Kiyotaka Toshimori ,  Chizuru Ito ,  Kenji Yamatoya ,  Keiichi Yoshida

发明人： Kiyotaka Toshimori ,  Chizuru Ito ,  Kenji Yamatoya ,  Keiichi Yoshida

IPC分类号： G01N33/53 ,  G01N33/577

CPC分类号： G01N33/5044 ,  G01N33/689 ,  G01N2500/00

摘要： A method of identifying compounds that inhibit fertilization is provided. The method can include selecting compounds that bind to equatorin protein. Two types of equatorin protein, a long form and a short form, can be present in the testis. The amino acid sequence of mouse equatorin from positions 101 to 146 including the 138th O-glycosylated threonine residue contains an epitope recognized by anti-equatorin antibody MN9 that has an effect of inhibiting fertilization. In addition, the MN9 antibody also binds to human sperm. Compounds that bind to the epitope can inhibit fertilization. Both forms of mouse equatorin can be used as well as human equatorin to identify compounds that inhibit fertilization.

摘要翻译： 提供了鉴定抑制受精的化合物的方法。 该方法可以包括选择结合赤铁蛋白的化合物。 睾丸中可以存在两种类型的赤道蛋白，长形式和短型。 含有第138位O-糖基化苏氨酸残基的101〜146位的小鼠赤尾蛋白的氨基酸序列含有抗赤道素抗体MN9所识别的具有抑制受精作用的表位。 此外，MN9抗体还结合人精子。 与表位结合的化合物可以抑制受精。 可以使用两种形式的小鼠赤尾蛋白以及人类赤道蛋白来鉴定抑制受精的化合物。