公开(公告)号：US5853718A

公开(公告)日：1998-12-29

申请号：US434353

申请日：1995-05-02

申请人： S.o slashed.ren Molin ,  Poul Kirketerp Andersson ,  Kenn Ax.o slashed. Gerdes ,  Per Klemm

发明人： S.o slashed.ren Molin ,  Poul Kirketerp Andersson ,  Kenn Ax.o slashed. Gerdes ,  Per Klemm

IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K14/245 ,  C12N1/00 ,  C12N15/00 ,  A61K39/02 ,  A61K39/002 ,  A61K39/108 ,  A61K39/12

CPC分类号： C07K14/245 ,  C12N15/00 ,  A61K39/00

摘要： A vertebrate animal, such as a human, is immunized with a bacterial host cell harboring a recombinant replicon which provides a stochastically expressed cell killing function whereby the cells are biologically contained. The replicon also comprises a gene encoding an antigen which is to be displayed on the outer surface of the host cell, so that it can elicit an immune response from the immunized animal. This antigen comprises one or more epitopes from a pathogenic agent.

摘要翻译： 脊椎动物，例如人，用携带重组复制子的细菌宿主细胞免疫，其提供随机表达的细胞杀伤功能，由此细胞被生物地包含。 复制子还包含编码要在宿主细胞的外表面上显示的抗原的基因，从而可以引起免疫动物的免疫应答。 该抗原包含来自病原体的一个或多个表位。

公开(公告)号：US5670370A

公开(公告)日：1997-09-23

申请号：US452494

申请日：1995-05-30

申请人： S.o slashed.ren Molin ,  Poul Kirketerp Andersson ,  Kenn Ax.o slashed. Gerdes ,  Per Klemm

发明人： S.o slashed.ren Molin ,  Poul Kirketerp Andersson ,  Kenn Ax.o slashed. Gerdes ,  Per Klemm

IPC分类号： C12N15/09 ,  A61K39/00 ,  C07K14/245 ,  C12N1/00 ,  C12N15/00 ,  C12P21/06

CPC分类号： C07K14/245 ,  C12N15/00 ,  A61K39/00

摘要： A replicon, in which a nucleotide sequence encoding a cell killing function is regulatably expressed when the replicon is harboured in one type of host cell (primary host cell), so that cells harbouring the replicon are killed under conditions under which the cell killing function is expressed, and the nucleotide sequence encoding the cell killing function is regulatably or constitutively expressed when the replicon is harboured in another type of host cell (secondary host cell), so that cells harbouring the replicon are invariably killed or killed under conditions under which the cell killing function is expressed, may be used in a method of active biological containment of cells under defined environmental conditions. The biological containment principle may be utilized in the industrial production of a biosynthetic product by recombinant DNA techniques, when deliberately releasing a genetically engineered microorganism to the natural environment or in the preparation of a live vaccine. The expression of the cell killing function may be regulated by means of a promoter.

摘要翻译： 当复制子存在于一种类型的宿主细胞（主要宿主细胞）中时，可复制表达编码细胞杀伤功能的核苷酸序列的复制子，使得携带复制子的细胞在细胞杀伤功能为 表达，并且当复制子宿主在另一种类型的宿主细胞（次级宿主细胞）中时，编码细胞杀伤功能的核苷酸序列是可调节的或组成型表达的，使得携带复制子的细胞在细胞的条件下总是被杀死或杀死 表达杀伤功能，可用于在确定的环境条件下对细胞的活性生物遏制的方法。 当将遗传工程微生物故意释放到天然环境或活疫苗的制备中时，可通过重组DNA技术在生物合成产物的工业生产中利用生物遏制原理。 细胞杀伤功能的表达可以通过启动子调节。