公开(公告)号：US08986951B2

公开(公告)日：2015-03-24

申请号：US13702792

申请日：2011-06-08

申请人： Andreas Hohlbaum ,  Alexandra Baehre ,  Gabriele Matschiner ,  Stefan Trentmann ,  Klaus Kirchfeld ,  Hans-Juergen Christian

发明人： Andreas Hohlbaum ,  Alexandra Baehre ,  Gabriele Matschiner ,  Stefan Trentmann ,  Klaus Kirchfeld ,  Hans-Juergen Christian

IPC分类号： C12P21/06 ,  C07K14/435 ,  C07K14/47 ,  C12N9/16 ,  A61K38/17 ,  C12N15/09 ,  C12P21/02

CPC分类号： A61K38/1709 ,  C07K14/435 ,  C07K14/47 ,  C07K2319/00 ,  C12N9/16

摘要： The present invention relates to novel muteins derived from human tear lipocalin, which bind to IL 4 receptor alpha. The sequences of the muteins comprise particular combinations of amino acids. In particular a mutated amino acid residue is present at any one or more of the sequence positions 27, 28, 30, 31, 33, 53, 57, 61, 64, 66, 80, 83, 104-106 and 108 of the linear polypeptide sequence of the mature human tear lipocalin. A mutated amino acid residue is also present at any 2 or more of the sequence positions 26, 32, 34, 55, 56, 58 and 63 of the linear polypeptide sequence of the mature human tear lipocalin. The invention also provides a corresponding nucleic acid molecule encoding such a mutein and a method for producing such a mutein and its encoding nucleic acid molecule.

摘要翻译： 本发明涉及衍生自与人IL-4受体α结合的人泪脂蛋白的新型突变蛋白。 突变蛋白的序列包含氨基酸的特定组合。 特别地，突变的氨基酸残基存在于线性的任何一个或多个序列位置27,28,30,31,33,53,57,61,64,66,80,83,104-106和108中的任何一个或多个 成熟人类脂质运载蛋白的多肽序列。 突变的氨基酸残基也存在于成熟人类泪液脂质运载蛋白的线性多肽序列的任何2个或更多个序列位置26,32,34,55,56,58和63。 本发明还提供了编码这种突变蛋白的相应核酸分子及其制备这种突变蛋白及其编码核酸分子的方法。

公开(公告)号：US20130085113A1

公开(公告)日：2013-04-04

申请号：US13702792

申请日：2011-06-08

申请人： Andreas Hohlbaum ,  Alexandra Baehre ,  Gabriele Matschiner ,  Stefan Trentmann ,  Klaus Kirchfeld ,  Hans-Juergen Christian

发明人： Andreas Hohlbaum ,  Alexandra Baehre ,  Gabriele Matschiner ,  Stefan Trentmann ,  Klaus Kirchfeld ,  Hans-Juergen Christian

IPC分类号： C07K14/435

CPC分类号： A61K38/1709 ,  C07K14/435 ,  C07K14/47 ,  C07K2319/00 ,  C12N9/16

摘要： The present invention relates to novel muteins derived from human tear lipocalin, which bind to IL 4 receptor alpha. The sequences of the muteins comprise particular combinations of amino acids. In particular a mutated amino acid residue is present at any one or more of the sequence positions 27, 28, 30, 31, 33, 53, 57, 61, 64, 66, 80, 83, 104-106 and 108 of the linear polypeptide sequence of the mature human tear lipocalin. A mutated amino acid residue is also present at any 2 or more of the sequence positions 26, 32, 34, 55, 56, 58 and 63 of the linear polypeptide sequence of the mature human tear lipocalin. The invention also provides a corresponding nucleic acid molecule encoding such a mutein and a method for producing such a mutein and its encoding nucleic acid molecule.

摘要翻译： 本发明涉及衍生自与人IL-4受体α结合的人泪脂蛋白的新型突变蛋白。 突变蛋白的序列包含氨基酸的特定组合。 特别地，突变的氨基酸残基存在于线性的任何一个或多个序列位置27,28,30,31,33,53,57,61,64,66,80,83,104-106和108中的任何一个或多个 成熟人类脂质运载蛋白的多肽序列。 突变的氨基酸残基也存在于成熟人类泪液脂质运载蛋白的线性多肽序列的任何2个或更多个序列位置26,32,34,55,56,58和63。 本发明还提供了编码这种突变蛋白的相应核酸分子及其制备这种突变蛋白及其编码核酸分子的方法。