Process for generating male sterile plants
    2.
    发明授权
    Process for generating male sterile plants 失效
    产生雄性不育植物的方法

    公开(公告)号:US06262339B1

    公开(公告)日:2001-07-17

    申请号:US08556944

    申请日:1996-04-02

    CPC classification number: C12N9/88 C12N15/8289

    Abstract: The invention is directed to a process for the generation of male sterility in plants comprising the steps of (a) transforming a plant cell with DNA sequences that selectively inhibit the expression of essential metabolic compounds and (b) regenerating plants from said plant cells. Cells impaired in the biosynthesis of basic metabolic compounds undergo starvation and eventually die. Such pathways include amino acid biosynthesis, nucleic acid biosynthesis and other biosynthetic pathways such as citric acid cycle, pentose phosphate pathway, fatty acid metabolism, vitamin biosynthesis that will render the cell inactive due to nutrient depletion, if one or more enzymes or proteins involved in this pathway would become inactive by using inhibitory DNA constructs.

    Abstract translation: 本发明涉及在植物中产生雄性不育的方法,包括以下步骤:(a)用选择性抑制必需代谢化合物表达的DNA序列转化植物细胞,和(b)从所述植物细胞再生植物。 碱性代谢化合物生物合成中受损的细胞经历饥饿并最终死亡。 这样的途径包括氨基酸生物合成,核酸生物合成和其他生物合成途径,例如柠檬酸循环,戊糖磷酸途径,脂肪酸代谢,维生素合成,如果参与的一种或多种酶或蛋白质会使细胞由于营养物质消耗而失活 通过使用抑制性DNA构建体,该途径将变得无活性。

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