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1.发明授权Method of inducing genome reorganization via intracellular activation of thermostable multifrequency DNA-cleaving enzyme 有权通过热稳定性多频DNA切割酶的细胞内活化诱导基因组重组的方法公开(公告)号：US07968339B2
公开(公告)日：2011-06-28
申请号：US11791126
申请日：2005-11-22
申请人： Kunihiro Ohta , Hidetaka Seo , Kouji Hirota , Takehiko Shibata
发明人： Kunihiro Ohta , Hidetaka Seo , Kouji Hirota , Takehiko Shibata
CPC分类号： C12N15/905 , C12N9/16 , C12N15/81
摘要： The present invention relates to a method for increasing genetic recombination frequency in a genomic DNA and a method for inducing genome rearrangement. Specifically, according to the present invention there are provided: the method for increasing genetic recombination frequency in a cell in which genetic recombination takes place at any sites in the genome, comprising causing a restriction enzyme to be expressed in the cell, inducing transient activation of the restriction enzyme, and then introducing 2 or more double strand cleavages into any genomic DNA of the cell, so as to increase the genetic recombination frequency; the method for inducing genome rearrangement through the use of the above method; and cells each prepared through the use of the above 2 methods.
摘要翻译： 本发明涉及提高基因组DNA中基因重组频率的方法和诱导基因组重排的方法。 具体地说，根据本发明，提供了在基因组中的任何位置发生遗传重组的细胞中增加遗传重组频率的方法，包括使限制性内切酶在细胞中表达，引起瞬时激活 限制酶，然后将2个以上的双链裂解引入细胞的任何基因组DNA，以增加遗传重组频率; 通过使用上述方法诱导基因组重排的方法; 每个细胞通过使用上述2种方法制备。
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2.发明申请Method of Inducing Genome Reorganization Via Intracellular Activation of Thermostable Multifrequency Dna-Cleaving Enzyme 有权通过热稳定多频Dna切割酶的细胞内激活诱导基因组重组的方法公开(公告)号：US20080166809A1
公开(公告)日：2008-07-10
申请号：US11791126
申请日：2005-11-22
申请人： Kunihiro Ohta , Hidetaka Seo , Kouji Hirota , Takehiko Shibata
发明人： Kunihiro Ohta , Hidetaka Seo , Kouji Hirota , Takehiko Shibata
CPC分类号： C12N15/905 , C12N9/16 , C12N15/81
摘要： The present invention relates to a method for increasing genetic recombination frequency in a genomic DNA and a method for inducing genome rearrangement. Specifically, according to the present invention there are provided: the method for increasing genetic recombination frequency in a cell in which genetic recombination takes place at any sites in the genome, comprising causing a restriction enzyme to be expressed in the cell, inducing transient activation of the restriction enzyme, and then introducing 2 or more double strand cleavages into any genomic DNA of the cell, so as to increase the genetic recombination frequency; the method for inducing genome rearrangement through the use of the above method; and cells each prepared through the use of the above 2 methods.
摘要翻译： 本发明涉及提高基因组DNA中基因重组频率的方法和诱导基因组重排的方法。 具体地说，根据本发明，提供了在基因组中的任何位置发生遗传重组的细胞中增加遗传重组频率的方法，包括使限制性内切酶在细胞中表达，引起瞬时激活 限制酶，然后将2个以上的双链裂解引入细胞的任何基因组DNA，以增加遗传重组频率; 通过使用上述方法诱导基因组重排的方法; 每个细胞通过使用上述2种方法制备。
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