公开(公告)号：US20100015643A1

公开(公告)日：2010-01-21

申请号：US12301535

申请日：2007-05-21

申请人： Chuwa Tei ,  Kouji Orihara ,  Kinya Nagata ,  Ryutaro Oba ,  Hiroyuki Hirai

发明人： Chuwa Tei ,  Kouji Orihara ,  Kinya Nagata ,  Ryutaro Oba ,  Hiroyuki Hirai

IPC分类号： G01N33/567

CPC分类号： G01N33/5091 ,  G01N33/5094 ,  G01N33/6893 ,  G01N2800/26

摘要： There is provided a method of quantitative determination using a flow cytometer, with which quantitative determination of cell surface protein can be effected more accurately than with current methods. The inventors have achieved the present invention by providing a method of quantitative determination of sites, per cell of a test sample, at which an antibody is bound to an antigen protein (sites/cell), characterized by preparing a calibration curve on the basis of fluorescent intensities obtained through measuring with a flow cytometer the amount of labeled antibodies against antigen protein which are bound to two or more groups of beads carrying known and different amounts of the antigen protein, and numeric values of the known amounts of the antigen protein, and further by measuring, with the flow cytometer, labeled antibodies against antigen protein after they have been reacted with test cells derived from a blood sample of a test subject, whereby digitalization is effected through comparison and conversion between the calibration curve and a fluorescence intensity obtained.

摘要翻译： 提供了一种使用流式细胞仪进行定量测定的方法，可以比目前的方法更准确地进行细胞表面蛋白的定量测定。 发明人通过提供定量测定抗体与抗原蛋白（位点/细胞）结合的测试样品的每个细胞的位点的方法来实现本发明，其特征在于基于以下方式制备校准曲线 通过用流式细胞仪测量获得的荧光强度，结合到携带已知和不同量的抗原蛋白的两个或更多个珠粒组的抗原蛋白的标记抗体的量，以及已知量的抗原蛋白的数值，以及 进一步通过用流式细胞仪测量抗原蛋白的抗体，在与测试受试者的血液样本的测试细胞反应之后，通过比较和转换校正曲线和获得的荧光强度来进行数字化。