公开(公告)号：US20100035335A1

公开(公告)日：2010-02-11

申请号：US12537544

申请日：2009-08-07

Applicant: Joseph R. Lakowicz ,  Henryk Szmacinski ,  Krishanu Ray ,  Mustafa Habib Chowdhury ,  Kazimierz Nowaczyk

Inventor： Joseph R. Lakowicz ,  Henryk Szmacinski ,  Krishanu Ray ,  Mustafa Habib Chowdhury ,  Kazimierz Nowaczyk

IPC: C12M1/34 ,  G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/648

Abstract: A method for enhancing fluorescence of a biomolecule includes the step of associating the biomolecule having intrinsic fluorescence with a sensing surface that contains nanostructured metal. Association of the biomolecule with the nanostructured metal enhances its intrinsic fluorescence, which is detected upon exposure to electromagnetic radiation of a suitable wavelength. The sensing surface may include capture or ligand molecule which binds to the biomolecule and sequesters it in proximity to the nanostructured metal, thereby causing its fluorescent signal to be enhanced. The method can be used in label-free bioassays for detection of interacting biomolecules, such as antibody-antigen binding.

Abstract translation: 用于增强生物分子荧光的方法包括将具有固有荧光的生物分子与含有纳米结构金属的感测表面缔合的步骤。 生物分子与纳米结构金属的结合增强其固有荧光，其在暴露于合适波长的电磁辐射时被检测。 感测表面可以包括与生物分子结合并将其螯合到接近纳米结构金属的俘获或配体分子，从而导致其荧光信号被增强。 该方法可用于无标记生物测定中，用于检测相互作用的生物分子，如抗体 - 抗原结合。

公开(公告)号：US20120228523A1

公开(公告)日：2012-09-13

申请号：US13508840

申请日：2009-11-09

Applicant: Krishnamurthy Manchikanti ,  Krishanu Ray ,  Ravindra Kumar Gattamaraju

Inventor： Krishnamurthy Manchikanti ,  Krishanu Ray ,  Ravindra Kumar Gattamaraju

IPC: H01J35/02 ,  G21G4/00

CPC classification number: H05G2/001

Abstract: The invention provides targets coated with structured biological materials, which are employed in laser produced plasma systems. The biological materials selected from cells of microbial, protozoan or plankton origin are applied on a portion of a solid target, like polished glass plate which then form a target system that absorbs the intense laser pulses, generates hot dense plasma and results in the emission of the X-rays. The method of coating structured biomaterial decreases the usable laser intensity required for producing the hot plasma, while increasing the X-ray yield. The coatings are easy to prepare and it is possible to vary the nature and shape of the cellular material in order to control/regulate the interaction with the light and thereby optimize the resultant plasma generation and X-ray emission. The increase in temperature of the plasma and the increase in yield demonstrate that the method is suitable for enhancing the emission yield in the Ultra Violet, Extreme Ultra violet, x-ray and the hard x-ray regimes.

Abstract translation: 本发明提供涂覆有结构化生物材料的靶，其用于激光制备的等离子体系统中。 从微生物，原生动物或浮游生物来源的细胞中选出的生物材料应用于固体靶的一部分，如抛光玻璃板，然后形成吸收强激光脉冲的目标系统，产生热致密的等离子体，并导致发射 X光片。 涂覆结构化生物材料的方法降低了生产热等离子体所需的可用激光强度，同时提高了X射线产率。 涂层易于制备，并且可以改变多孔材料的性质和形状，以便控制/调节与光的相互作用，从而优化所得的等离子体产生和X射线发射。 等离子体的温度升高和产量的增加表明，该方法适用于提高超紫，极紫外，X射线和硬x射线体系中的发射产率。