公开(公告)号：US20060183111A1

公开(公告)日：2006-08-17

申请号：US11334392

申请日：2006-01-19

申请人： Ajit Kumar ,  Krishna Banaudha

发明人： Ajit Kumar ,  Krishna Banaudha

IPC分类号： C12Q1/70 ,  C12N5/08

CPC分类号： C12N7/00 ,  C12N2770/24251

摘要： Novel methods are disclosed for the long-term culture of human primary hepatocytes in tissue culture system. This culture system supports the replication, gene expression and dissemination of a multitude of pathogenic agents that reside in, or pass through, the liver. Among these, the invention advantageously provides for efficient hepatitis C virus (HCV) replication, and dissemination. Additionally disclosed are methods for the determining cellular receptors, hepatocyte cell subtypes, and host cell molecules required for efficient hepatic pathogen replication, expression and dissemination.

摘要翻译： 公开了组织培养系统中人原代肝细胞长期培养的新方法。 该培养系统支持多种病原体的复制，基因表达和传播，其存在于肝脏或通过肝脏。 其中，本发明有利地提供有效的丙型肝炎病毒（HCV）复制和传播。 另外公开了确定有效的肝病原体复制，表达和传播所需的细胞受体，肝细胞细胞亚型和宿主细胞分子的方法。