公开(公告)号：US06448051B1

公开(公告)日：2002-09-10

申请号：US09747498

申请日：2001-03-15

申请人： Kulbhussan Balwant Bastawade ,  Digambar Vitthal Gokhale ,  Ravindranathan Thottapillil ,  Sandeep Raghunath Ghorpade ,  Rohini Ramesh Joshi ,  Uttam Ramrao Kalkote

发明人： Kulbhussan Balwant Bastawade ,  Digambar Vitthal Gokhale ,  Ravindranathan Thottapillil ,  Sandeep Raghunath Ghorpade ,  Rohini Ramesh Joshi ,  Uttam Ramrao Kalkote

IPC分类号： C12P762

CPC分类号： C12P7/62 ,  C12P41/004 ,  Y10S435/917 ,  Y10S435/942

摘要： The present invention relates to a process for the preparation of 4-(R)-hydroxycyclopent-2-en-1 (S)-acetate of the formula 2 by reacting meso-cyclopent-2-en-1, 4-diacetate of formula 1 with a whole cell enzyme in a mixture of a buffer and an organic solvent, filtering the reaction mixture to remove the enzyme, extracting the resultant compound with an organic solvent, and removing the solvent to obtain the desired product.

摘要翻译： 本发明涉及通过使式1的内消旋 - 环戊-2-烯-1,4-二乙酸酯与式（Ⅰ）的4-（R） - 羟基环戊-2-烯-1 在缓冲液和有机溶剂的混合物中的全细胞酶，过滤反应混合物以除去酶，用有机溶剂萃取所得化合物，并除去溶剂以获得所需产物。