公开(公告)号：US07092154B2

公开(公告)日：2006-08-15

申请号：US10432396

申请日：2001-11-22

申请人： Kenji Yasuda ,  Kunihiko Kaneko ,  Tetsuya Yomo ,  Ippei Inoue ,  Yuichi Wakamoto

发明人： Kenji Yasuda ,  Kunihiko Kaneko ,  Tetsuya Yomo ,  Ippei Inoue ,  Yuichi Wakamoto

IPC分类号： G01N21/01 ,  G02B21/34

CPC分类号： C12M25/02 ,  B01L3/502 ,  C12M25/00 ,  C12M41/36 ,  C12M41/46 ,  G01N35/028 ,  G01N35/1002 ,  G02B21/34

摘要： An apparatus is provided with a cell culture container having a cell culture region made of a hole formed on a substrate, a semi-permeable membrane covering a top plane of the cell culture region and a culture medium replacement part provided over the semi-permeable membrane, a mechanism for supplying a cell culture medium into the cell culture container, and a microscopic optical mechanism for enabling long-term observation of the cell within the cell culture region. This apparatus makes it possible to culture a cell group originating from a particular single cell, to perform culture and observation while identifying cells to be subjected to interaction during the process of culturing cells, and observe a difference in variation between the particular cell and other cells. There is also provided a mechanism which makes it possible to collect only a cell assuming a particular state and perform analysis or biochemical measurement of a gene of the cell, an expressed mRNA and the like.

摘要翻译： 本发明提供一种细胞培养容器，该细胞培养容器具有由形成在基材上的孔形成的细胞培养区域，覆盖细胞培养区域的顶面的半透膜和设置在半透膜上的培养基置换部件 用于向细胞培养容器供给细胞培养基的机理，以及能够长期观察细胞培养区域内的细胞的微观光学机构。 该装置可以培养来自特定单细胞的细胞群，进行培养和观察，同时确定在培养细胞过程中待相互作用的细胞，并观察特定细胞与其它细胞之间的差异 。 还提供了一种使得可以仅收集假设特定状态的细胞并进行细胞基因，表达的mRNA等的分析或生物化学测量的机制。