公开(公告)号：US09139807B2

公开(公告)日：2015-09-22

申请号：US14130122

申请日：2012-07-13

申请人： Yuichi Wakamoto ,  Mikihiro Hashimoto

发明人： Yuichi Wakamoto ,  Mikihiro Hashimoto

IPC分类号： C12M1/00 ,  C12Q1/06 ,  C12Q1/24 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/02 ,  C12M3/06 ,  C12M1/12

CPC分类号： C12M41/46 ,  C12M23/16 ,  C12M23/22 ,  C12M23/34 ,  C12M23/40 ,  C12M25/02 ,  C12M29/10 ,  C12M41/36 ,  C12Q1/02 ,  C12Q1/045

摘要： Provided is a cell culture apparatus, an apparatus for long-term observation of cell culture, a method for long-term cell culture, and a method for long-term observation of cell culture, which are capable of continuously culturing and observing cells under uniform environmental conditions without aging of the cultured cells' physiological state, and also tracing the history of a cell. The cell culture apparatus comprises a cell culture substrate, a semipermeable membrane, and a culture solution supplier. The cell culture substrate has on a surface a narrow culture groove for holding and culturing cells and wide flow grooves for discarding the cells held and cultured in the culture groove. Both ends of the culture groove are connected to the flow grooves being larger in width and depth than the culture groove. The semipermeable membrane covers the culture groove and the flow grooves. Finally, the culture solution supplier continuously supplies culture solution.

摘要翻译： 本发明提供细胞培养装置，细胞培养的长期观察装置，长期细胞培养方法和长期观察细胞培养方法，能够连续培养和观察均匀的细胞 环境条件下不培养细胞的生理状态老化，也追踪细胞的病史。 细胞培养装置包括细胞培养基质，半透膜和培养液供给体。 细胞培养基底表面具有用于保持和培养细胞的窄培养槽和用于丢弃在培养槽中保持和培养的细胞的宽流槽。 培养槽的两端连接到比培养槽宽的和深度更大的流动槽。 半透膜覆盖培养槽和流槽。 最后，文化解决方案供应商不断提供文化解决方案。

公开(公告)号：US20140147879A1

公开(公告)日：2014-05-29

申请号：US14130122

申请日：2012-07-13

申请人： Yuichi Wakamoto ,  Mikihiro Hashimoto

发明人： Yuichi Wakamoto ,  Mikihiro Hashimoto

IPC分类号： C12M1/34 ,  C12Q1/02

CPC分类号： C12M41/46 ,  C12M23/16 ,  C12M23/22 ,  C12M23/34 ,  C12M23/40 ,  C12M25/02 ,  C12M29/10 ,  C12M41/36 ,  C12Q1/02 ,  C12Q1/045

摘要： To provide a cell culture apparatus, an apparatus for long-term observation of cell culture, a method for long-term cell culture, and a method for long-term observation of cell culture, which are capable of continuously culturing and observing cells under a uniform environmental condition without variation in physiological state associated with aging of the cultured cells, and also tracing the history (genealogy) of a certain cell.A cell culture apparatus comprising a cell culture substrate, a semipermeable membrane, and a culture solution supply means, in which the cell culture substrate has, on a surface thereof, a narrow culture groove for holding and culturing cells and wide flow grooves for discarding the cells held and cultured in the culture groove, the both ends of the culture groove being connected to the flow grooves and the flow grooves being larger in width and depth than the culture groove; the semipermeable membrane is used so as to cover the culture groove and the flow grooves of the cell culture substrate; and the culture solution supply means is capable of continuously supplying culture solution to the cell culture substrate covered with the semipermeable membrane.

摘要翻译： 为了提供细胞培养装置，用于长期观察细胞培养的装置，长期细胞培养的方法和长期观察细胞培养的方法，其能够连续培养和观察细胞培养下的细胞 均匀的环境条件，与培养细胞衰老相关的生理状态没有变化，并且还追踪特定细胞的历史（家谱）。 一种细胞培养装置，包括细胞培养基材，半透膜和培养液供给机构，其中，细胞培养基材的表面具有用于保持培养细胞的狭窄培养槽和宽流槽， 细胞在培养槽中保持和培养，培养槽的两端与流槽相连，流槽在宽度和深度上比培养槽大; 使用半透膜以覆盖细胞培养基板的培养槽和流槽; 并且培养液供给装置能够将培养液连续供给到被半透膜覆盖的细胞培养基材上。

公开(公告)号：US07092154B2

公开(公告)日：2006-08-15

申请号：US10432396

申请日：2001-11-22

申请人： Kenji Yasuda ,  Kunihiko Kaneko ,  Tetsuya Yomo ,  Ippei Inoue ,  Yuichi Wakamoto

发明人： Kenji Yasuda ,  Kunihiko Kaneko ,  Tetsuya Yomo ,  Ippei Inoue ,  Yuichi Wakamoto

IPC分类号： G01N21/01 ,  G02B21/34

CPC分类号： C12M25/02 ,  B01L3/502 ,  C12M25/00 ,  C12M41/36 ,  C12M41/46 ,  G01N35/028 ,  G01N35/1002 ,  G02B21/34

摘要： An apparatus is provided with a cell culture container having a cell culture region made of a hole formed on a substrate, a semi-permeable membrane covering a top plane of the cell culture region and a culture medium replacement part provided over the semi-permeable membrane, a mechanism for supplying a cell culture medium into the cell culture container, and a microscopic optical mechanism for enabling long-term observation of the cell within the cell culture region. This apparatus makes it possible to culture a cell group originating from a particular single cell, to perform culture and observation while identifying cells to be subjected to interaction during the process of culturing cells, and observe a difference in variation between the particular cell and other cells. There is also provided a mechanism which makes it possible to collect only a cell assuming a particular state and perform analysis or biochemical measurement of a gene of the cell, an expressed mRNA and the like.

摘要翻译： 本发明提供一种细胞培养容器，该细胞培养容器具有由形成在基材上的孔形成的细胞培养区域，覆盖细胞培养区域的顶面的半透膜和设置在半透膜上的培养基置换部件 用于向细胞培养容器供给细胞培养基的机理，以及能够长期观察细胞培养区域内的细胞的微观光学机构。 该装置可以培养来自特定单细胞的细胞群，进行培养和观察，同时确定在培养细胞过程中待相互作用的细胞，并观察特定细胞与其它细胞之间的差异 。 还提供了一种使得可以仅收集假设特定状态的细胞并进行细胞基因，表达的mRNA等的分析或生物化学测量的机制。