公开(公告)号：US07803623B2

公开(公告)日：2010-09-28

申请号：US12261166

申请日：2008-10-30

申请人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

发明人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

IPC分类号： C12N15/87 ,  C12N1/21

CPC分类号： C12N15/52 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  Y02E50/16 ,  Y02E50/17

摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.

摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。

公开(公告)号：US07897396B2

公开(公告)日：2011-03-01

申请号：US12261160

申请日：2008-10-30

申请人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

发明人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

IPC分类号： C12N15/74 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12P7/065 ,  Y02E50/17

摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.

摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。

公开(公告)号：US20090221078A1

公开(公告)日：2009-09-03

申请号：US12261166

申请日：2008-10-30

申请人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

发明人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

IPC分类号： C12N15/87 ,  C12N1/21

CPC分类号： C12N15/52 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  Y02E50/16 ,  Y02E50/17

摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.

摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。

公开(公告)号：US20090203099A1

公开(公告)日：2009-08-13

申请号：US12261160

申请日：2008-10-30

申请人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

发明人： Perry G. Caimi ,  Yat-Chen Chou ,  Mary Ann Franden ,  Kyle Knoke ,  Luan Tao ,  Paul V. Viitanen ,  Min Zhang ,  Yuying Zhang

IPC分类号： C12P7/06

CPC分类号： C12P7/065 ,  Y02E50/17

摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.

摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。