公开(公告)号：US07192757B2

公开(公告)日：2007-03-20

申请号：US10884325

申请日：2004-07-02

申请人： Mads Nørregard-Madsen ,  Line Bloch Larsen ,  Peter Kamp Hansen

发明人： Mads Nørregard-Madsen ,  Line Bloch Larsen ,  Peter Kamp Hansen

IPC分类号： C12N9/52 ,  C12N9/54 ,  C12N15/57 ,  C12N15/74 ,  C11D3/386

CPC分类号： C12N9/54 ,  C11D3/38681

摘要： The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42–43, 51–56, 155–161, 187–190, 216–217, 217–218 or 218–219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.

摘要翻译： 本发明涉及具有减少鸡蛋中存在的物质的抑制倾向的减毒酶变体，例如IV-0型胰蛋白酶抑制剂。 特别地，所述变体包含位置42-43,51-56,155-161,187-190,216-217,217-218或218-219之间的至少一个另外的氨基酸残基（以BASBPN编号）。 这些枯草芽孢杆菌变体在用于例如蛋白质时在蛋渍上表现出优异或改善的洗涤性能是有用的。 清洁剂或洗涤剂组合物，例如衣物洗涤剂组合物和洗涤剂组合物，包括自动洗碗剂组合物。 而且，编码变体，分离的载体，宿主细胞的分离的DNA序列和用于产生和使用本发明的变体的方法。 此外，公开了包含变体的清洁剂和洗涤剂组合物。

公开(公告)号：US06893855B2

公开(公告)日：2005-05-17

申请号：US09976414

申请日：2001-10-12

申请人： Mads Nørregaard-Madsen ,  Line Bloch Larsen ,  Peter Kamp Hansen

发明人： Mads Nørregaard-Madsen ,  Line Bloch Larsen ,  Peter Kamp Hansen

IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N9/56 ,  C12N15/57 ,  C12N15/74

CPC分类号： C12N9/54 ,  C11D3/38681

摘要： The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42-43, 51-56, 155-161, 187-190, 216-217, 217-218 or 218-219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.

摘要翻译： 本发明涉及具有减少鸡蛋中存在的物质的抑制倾向的减毒酶变体，例如IV-0型胰蛋白酶抑制剂。 特别地，所述变体包含位置42-43,51-56,155-161,187-190,216-217,217-218或218-219之间的至少一个另外的氨基酸残基（以BASBPN编号）。 这些枯草芽孢杆菌变体在用于例如蛋白质时在蛋渍上表现出优异或改善的洗涤性能是有用的。 清洁剂或洗涤剂组合物，例如衣物洗涤剂组合物和洗涤剂组合物，包括自动洗碗剂组合物。 而且，编码变体，分离的载体，宿主细胞的分离的DNA序列和用于产生和使用本发明的变体的方法。 此外，公开了包含变体的清洁剂和洗涤剂组合物。