公开(公告)号：US06737255B2

公开(公告)日：2004-05-18

申请号：US09761782

申请日：2001-01-18

申请人： Vitaliy Arkadyevich Livshits ,  Vera Georgievna Doroshenko ,  Nataliya Vasilyevna Gorshkova ,  Alla Valentinovna Belaryeva ,  Lirina Valeryevna Ivanovskaya ,  Evgeni Moiseevich Khourges ,  Valery Zavenovich Akhverdian ,  Mikhail Markovich Gusyatiner ,  Yury Ivanovich Kozlov

发明人： Vitaliy Arkadyevich Livshits ,  Vera Georgievna Doroshenko ,  Nataliya Vasilyevna Gorshkova ,  Alla Valentinovna Belaryeva ,  Lirina Valeryevna Ivanovskaya ,  Evgeni Moiseevich Khourges ,  Valery Zavenovich Akhverdian ,  Mikhail Markovich Gusyatiner ,  Yury Ivanovich Kozlov

IPC分类号： C12P1308

CPC分类号： C12N9/93 ,  C12N9/88 ,  C12P13/08

摘要： The present invention provides an isolated DNA molecule encoding a small subunit of acetohydroxy acid synthase isozyme III originating from Escherichia coli and mutants of Escherichia coli acetohydroxy acid synthase isozyme III, which are free from inhibition by L-valine an can catalyze the conversion of: (a) pyruvate to &agr;-acetolactate and (b) &agr;-ketobutyrate and pyruvate to &agr;-aceto-a-hydroxybutyrate. The present invention also provides methods for producing L-valine by fermentation of a bacterium harboring the novel DNA molecule and/or expressing the mutant acetohydroxy acid synthase isozyme III.

摘要翻译： 本发明提供了编码源自大肠杆菌的乙酰羟酸合酶同功酶III的小亚基的分离的DNA分子，并且没有L-缬氨酸的抑制的大肠杆菌乙酰羟酸合酶同功酶III的突变体可以催化转化： a）丙酮酸至α-乙酰乳酸和（b）α-酮丁酸和丙酮酸至α-乙酰-α-羟基丁酸。 本发明还提供了通过发酵含有新的DNA分子的细菌和/或表达突变型乙酰羟酸合酶同功酶III来生产L-缬氨酸的方法。