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1.发明授权Method for producing an amino acid using a bacterium overexpressing an rhtC gene 有权使用过表达rhtC基因的细菌产生氨基酸的方法公开(公告)号：US09394346B2
公开(公告)日：2016-07-19
申请号：US11106455
申请日：2005-04-15
申请人： Vitaliy Arkadyevich Livshits , Natalia Pavlovna Zakataeva , Vladimir Veniaminovich Aleshin , Alla Valentinovna Belareva , Irina Lyvovna Tokhmakova
发明人： Vitaliy Arkadyevich Livshits , Natalia Pavlovna Zakataeva , Vladimir Veniaminovich Aleshin , Alla Valentinovna Belareva , Irina Lyvovna Tokhmakova
IPC分类号： C12P13/06 , C12P13/08 , C07K14/245
CPC分类号： C12P13/08 , C07K14/245 , C12P13/06
摘要： The present invention describes a bacterium which has an ability to produce an amino acid and in which the rhtC gene encoding a protein having an enhanced activity of imparting L-threonine resistance to a bacterium expressing the protein. Preferably, the bacterium further includes an rhtB gene encoding for a protein having an enhanced activity of imparting to a bacterium L-homoserine resistance expressing the protein. The present invention also describes a method of cultivating the bacterium in a culture medium to produce and accumulate amino acids in the medium, and the amino acid is recovered from the medium.
摘要翻译： 本发明描述了具有产生氨基酸的能力的细菌，其中编码具有增强的表达蛋白质的细菌的L-苏氨酸抗性活性的蛋白质的rhtC基因。 优选地，细菌还包括编码具有增强的表达蛋白质的L-高丝氨酸抗性细菌活性的蛋白质的rhtB基因。 本发明还描述了在培养基中培养细菌以在培养基中产生和积累氨基酸的方法，并且从培养基中回收氨基酸。
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2.发明授权Method for producing a lower alkyl ester of α-L-aspartyl-L-phenylalanine using Escherichia bacteria 有权使用大肠杆菌生产α-L-天冬氨酰-L-苯丙氨酸的低级烷基酯的方法公开(公告)号：US07531332B2
公开(公告)日：2009-05-12
申请号：US11761465
申请日：2007-06-12
申请人： Vitaliy Arkadyevich Livshits , Maria Viacheslavovna Vitushkina , Mikhail Markovich Gusyatiner , Mikhail Kharisovich Ziyatdinov , Valery Zavenovich Akhverdian , Ekaterina Alekseevna Savrasova , Vera Georgievna Doroshenko , Sergey Vladimirovich Mashko
发明人： Vitaliy Arkadyevich Livshits , Maria Viacheslavovna Vitushkina , Mikhail Markovich Gusyatiner , Mikhail Kharisovich Ziyatdinov , Valery Zavenovich Akhverdian , Ekaterina Alekseevna Savrasova , Vera Georgievna Doroshenko , Sergey Vladimirovich Mashko
CPC分类号： C07K14/245 , C07K5/0613 , C12P13/04 , C12P13/08 , C12P13/222
摘要： A method for producing an L-amino acid, such as L-phenylalanine and L-threonine, is provided using an Escherichia bacterium, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing the activity of the protein encoded by the yedA gene. A further method is provided for producing a lower alkyl ester of α-L-aspartyl-L-phenylalanine by producing L-phenylalanine by cultivating an Escherichia coli bacterium in a medium, wherein said bacterium has the ability to produce L-phenylalanine, and synthesizing the lower alkyl ester of α-L-aspartyl-L-phenylalanine from aspartic acid, or a derivative thereof, and the produced L-phenylalanine.
摘要翻译： 使用埃希氏菌属细菌提供L-苯丙氨酸和L-苏氨酸生产L-氨基酸的方法，其中所述细菌的L-氨基酸生产力通过提高由yedA编码的蛋白质的活性来增强 基因。 提供了通过在培养基中培养大肠杆菌细菌产生L-苯丙氨酸来生产α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯的另一种方法，其中所述细菌具有产生L-苯丙氨酸的能力，并合成 来自天冬氨酸的α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯或其衍生物和所产生的L-苯丙氨酸。
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公开(公告)号：US20080153138A1
公开(公告)日：2008-06-26
申请号：US11761465
申请日：2007-06-12
申请人： Vitaliy Arkadyevich Livshits , Maria Viacheslavovna Vitushkina , Mikhail Markovich Gusyatiner , Mikhail Kharisovich Ziyatdinov , Valery Zavenovich Akhverdian , Ekaterina Alekseevna Savrasova , Vera Georgievna Doroshenko , Sergey Vladimirovich Mashko
发明人： Vitaliy Arkadyevich Livshits , Maria Viacheslavovna Vitushkina , Mikhail Markovich Gusyatiner , Mikhail Kharisovich Ziyatdinov , Valery Zavenovich Akhverdian , Ekaterina Alekseevna Savrasova , Vera Georgievna Doroshenko , Sergey Vladimirovich Mashko
CPC分类号： C07K14/245 , C07K5/0613 , C12P13/04 , C12P13/08 , C12P13/222
摘要： A method for producing an L-amino acid, such as L-phenylalanine and L-threonine, is provided using an Escherichia bacterium, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing the activity of the protein encoded by the yedA gene. A further method is provided for producing a lower alkyl ester of α-L-aspartyl-L-phenylalanine by producing L-phenylalanine by cultivating an Escherichia coli bacterium in a medium, wherein said bacterium has the ability to produce L-phenylalanine, and synthesizing the lower alkyl ester of α-L-aspartyl-L-phenylalanine from aspartic acid, or a derivative thereof, and the produced L-phenylalanine.
摘要翻译： 使用埃希氏菌属细菌提供L-苯丙氨酸和L-苏氨酸生产L-氨基酸的方法，其中所述细菌的L-氨基酸生产力通过提高由yedA编码的蛋白质的活性来增强 基因。 提供了通过在培养基中培养大肠杆菌细菌产生L-苯丙氨酸来生产α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯的另一种方法，其中所述细菌具有生产L-苯丙氨酸的能力，并合成 来自天冬氨酸的α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯或其衍生物和所产生的L-苯丙氨酸。
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4.发明授权Escherichia bacteria transformed with a yedA homolog to enhance L-amino acid producing activity, and methods for producing an L-amino acid using same 有权用yedA同源物转化的埃希氏菌属细菌以增强L-氨基酸产生活性，以及使用其生产L-氨基酸的方法公开(公告)号：US07259003B2
公开(公告)日：2007-08-21
申请号：US10302983
申请日：2002-11-25
申请人： Vitaliy Arkadyevich Livshits , Maria Viacheslavovna Vitushkina , Mikhail Markovich Gusyatiner , Mikhail Kharisovich Ziyatdinov , Valery Zavenovich Akhverdian , Ekaterina Alekseevna Savrasova , Vera Georgievna Doroshenko , Sergey Vladimirovich Mashko
发明人： Vitaliy Arkadyevich Livshits , Maria Viacheslavovna Vitushkina , Mikhail Markovich Gusyatiner , Mikhail Kharisovich Ziyatdinov , Valery Zavenovich Akhverdian , Ekaterina Alekseevna Savrasova , Vera Georgievna Doroshenko , Sergey Vladimirovich Mashko
IPC分类号： C12N1/21 , C12P13/04 , C12P13/22 , C12P13/08 , C12N15/00 , C12P21/06 , C07K14/00 , C07H21/04
CPC分类号： C07K14/245 , C07K5/0613 , C12P13/04 , C12P13/08 , C12P13/222
摘要： A method for producing an L-amino acid, such as L-phenylalanine or L-threonine, is provided using a bacterium belonging to the genus Escherichia, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of the protein encoded by the yedA gene.
摘要翻译： 使用属于埃希氏杆菌属的细菌提供L-苯丙氨酸或L-苏氨酸生产L-氨基酸的方法，其中所述细菌的L-氨基酸生产力通过提高蛋白质的活性而增强 由yedA基因编码。
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5.发明授权Method for producing a lower alkyl ester of α-L-aspartyl-L-phenylalanine 有权制备α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯的方法公开(公告)号：US07935506B2
公开(公告)日：2011-05-03
申请号：US12569335
申请日：2009-09-29
申请人： Maria Viacheslavovna Vitushkina , Vitaliy Arkadyevich Livshits , Sergei Vladimirovich Mashko , Vera Georgievna Doroshenko , Irina Vladimirovna Biryukova , Zhanna Iosifovna Katashkina , Aleksandra Yurievna Skorokhodova , Alla Valentinovna Belareva
发明人： Maria Viacheslavovna Vitushkina , Vitaliy Arkadyevich Livshits , Sergei Vladimirovich Mashko , Vera Georgievna Doroshenko , Irina Vladimirovna Biryukova , Zhanna Iosifovna Katashkina , Aleksandra Yurievna Skorokhodova , Alla Valentinovna Belareva
IPC分类号： C12P13/22
CPC分类号： C12P13/222 , C07K14/245 , C12P13/227 , C12P21/02
摘要： A method for producing lower alkyl ester of α-L-aspartyl-L-phenylalanine by cultivating a recombinant Escherichia coli bacterium that has the ability to produce and accumulate an aromatic L-amino acid according in a culture medium to produce and accumulate L-phenylalanine in the medium and synthesizing lower alkyl ester of α-L-aspartyl-L-phenylalanine from the aspartic acid or its derivative and the obtained L-phenylalanine.
摘要翻译： 通过培养能够在培养基中产生并积累芳香族L-氨基酸的重组大肠杆菌细菌来生产和积累L-苯丙氨酸的生产α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯的方法 在培养基中合成α-L-天冬氨酰-L-苯丙氨酸的低级烷基酯，得到L-苯丙氨酸。
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6.发明授权Method of producing amino acids using E. coli transformed with sucrose PTS genes 有权使用用蔗糖PTS基因转化的大肠杆菌生产氨基酸的方法公开(公告)号：US07179623B2
公开(公告)日：2007-02-20
申请号：US11204011
申请日：2005-08-16
申请人： Vitaliy Arkadyevich Livshits , Vera Georgievna Doroshenko , Sergei Vladimirovich Mashko , Valery Zavenovich Akhverdian , Yury Ivanovich Kozlov
发明人： Vitaliy Arkadyevich Livshits , Vera Georgievna Doroshenko , Sergei Vladimirovich Mashko , Valery Zavenovich Akhverdian , Yury Ivanovich Kozlov
CPC分类号： C12N9/1205 , C12N15/52 , C12P13/04
摘要： Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.
摘要翻译： 氨基酸如苏氨酸，高丝氨酸，异亮氨酸，赖氨酸，缬氨酸和色氨酸使用属于埃希氏杆菌属的细菌产生，该细菌由属于埃希氏杆菌属的蔗糖非同化菌株构建，并含有蔗糖非PTS或PTS 基因并具有产生氨基酸的能力。
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7.发明授权公开(公告)号：US06960455B2
公开(公告)日：2005-11-01
申请号：US09841609
申请日：2001-04-25
申请人： Vitaliy Arkadyevich Livshits , Vera Georgievna Doroshenko , Sergei Vlsdimirovich Mashko , Valery Zavenovich Akhverdian , Yury Ivanovich Kozlov
发明人： Vitaliy Arkadyevich Livshits , Vera Georgievna Doroshenko , Sergei Vlsdimirovich Mashko , Valery Zavenovich Akhverdian , Yury Ivanovich Kozlov
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N15/52 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/22 , C12R1/19
CPC分类号： C12N9/1205 , C12N15/52 , C12P13/04
摘要： An amino acid such as threonine, homoserine, isoleucine, lysine, valine and tryptophan is produced using a bacterium belonging to the genus Escherichia which has been constructed from sucorse non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS (phosphoenol pyruvate-dependent sucrose-6-phosphotransferase system) genes and has an ability to produce the amino acid.
摘要翻译： 氨基酸例如苏氨酸，高丝氨酸，异亮氨酸，赖氨酸，缬氨酸和色氨酸是使用属于埃希氏杆菌属的属于埃希氏杆菌属的非同源性菌株构建的并且含有蔗糖非PTS（磷酸烯醇 丙酮酸依赖性蔗糖-6-磷酸转移酶系统）基因，并具有产生氨基酸的能力。
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8.发明申请Method for producing L-amino acids using bacteria belonging to the genus escherichia 有权使用属于埃希氏菌属的细菌生产L-氨基酸的方法公开(公告)号：US20100099153A1
公开(公告)日：2010-04-22
申请号：US12569335
申请日：2009-09-29
申请人： Maria Viacheslavovna Vitushkina , Vitaliy Arkadyevich Livshits , Sergei Vladimirovich Mashko , Vera Georgievna Doroshenko , Irina Vladimirovna Biryukova , Zhanna Iosifovna Katashkina , Aleksandra Yurievna Skorokhodova , Alla Valentinovna Belareva
发明人： Maria Viacheslavovna Vitushkina , Vitaliy Arkadyevich Livshits , Sergei Vladimirovich Mashko , Vera Georgievna Doroshenko , Irina Vladimirovna Biryukova , Zhanna Iosifovna Katashkina , Aleksandra Yurievna Skorokhodova , Alla Valentinovna Belareva
IPC分类号： C12P13/22
CPC分类号： C12P13/222 , C07K14/245 , C12P13/227 , C12P21/02
摘要： A method for producing lower alkyl ester of α-L-aspartyl-L-phenylalanine by cultivating a recombinant Escherichia coli bacterium that has the ability to produce and accumulate an aromatic L-amino acid according in a culture medium to produce and accumulate L-phenylalanine in the medium and synthesizing lower alkyl ester of α-L-aspartyl-L-phenylalanine from the aspartic acid or its derivative and the obtained L-phenylalanine.
摘要翻译： 通过培养能够在培养基中产生并积累芳香族L-氨基酸的重组大肠杆菌细菌来生产和积累L-苯丙氨酸的生产α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯的方法 在培养基中合成α-L-天冬氨酰-L-苯丙氨酸的低级烷基酯，得到L-苯丙氨酸。
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9.发明授权Escherichia bacteria transformed with the yddG gene to enhance L-amino acid producing activity 有权用yddG基因转化的大肠杆菌细菌以增强L-氨基酸产生活性公开(公告)号：US07666655B2
公开(公告)日：2010-02-23
申请号：US10302997
申请日：2002-11-25
申请人： Maria Viacheslavovna Vitushkina , Vitaliy Arkadyevich Livshits , Sergei Vladimirovich Mashko , Vera Georgievna Doroshenko , Irina Vladimirovna Biryukova , Zhanna Iosifovna Katashkina , Aleksandra Yurievna Skorokhodova , Alla Valentinovna Belareva
发明人： Maria Viacheslavovna Vitushkina , Vitaliy Arkadyevich Livshits , Sergei Vladimirovich Mashko , Vera Georgievna Doroshenko , Irina Vladimirovna Biryukova , Zhanna Iosifovna Katashkina , Aleksandra Yurievna Skorokhodova , Alla Valentinovna Belareva
CPC分类号： C12P13/222 , C07K14/245 , C12P13/227 , C12P21/02
摘要： The present invention relates to a method for producing L-amino acid, such as L-phenylalanine and L-tryptophan, is provided using bacterium belonging to the genus Escherichia wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of protein encoded by the yddG gene from Escherichia coli, wherein said protein has an activity to make said bacterium resistant to L-phenylalanine, a phenylalanine analogue, or a tryptophan analogue. The present invention further relates to a method for producing lower alkyl ester of α-L-aspartyl-L-phenylalanine.
摘要翻译： 本发明涉及使用属于埃希氏杆菌属的细菌的L-苯丙氨酸和L-色氨酸的L-氨基酸的生产方法，其中所述细菌的L-氨基酸生产力通过提高所述细菌的活性而提高 由来自大肠杆菌的yddG基因编码的蛋白质，其中所述蛋白质具有使所述细菌对L-苯丙氨酸，苯丙氨酸类似物或色氨酸类似物具有抗性的活性。 本发明还涉及α-L-天冬氨酰基-L-苯丙氨酸的低级烷基酯的制造方法。
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公开(公告)号：US06737255B2
公开(公告)日：2004-05-18
申请号：US09761782
申请日：2001-01-18
申请人： Vitaliy Arkadyevich Livshits , Vera Georgievna Doroshenko , Nataliya Vasilyevna Gorshkova , Alla Valentinovna Belaryeva , Lirina Valeryevna Ivanovskaya , Evgeni Moiseevich Khourges , Valery Zavenovich Akhverdian , Mikhail Markovich Gusyatiner , Yury Ivanovich Kozlov
发明人： Vitaliy Arkadyevich Livshits , Vera Georgievna Doroshenko , Nataliya Vasilyevna Gorshkova , Alla Valentinovna Belaryeva , Lirina Valeryevna Ivanovskaya , Evgeni Moiseevich Khourges , Valery Zavenovich Akhverdian , Mikhail Markovich Gusyatiner , Yury Ivanovich Kozlov
IPC分类号： C12P1308
摘要： The present invention provides an isolated DNA molecule encoding a small subunit of acetohydroxy acid synthase isozyme III originating from Escherichia coli and mutants of Escherichia coli acetohydroxy acid synthase isozyme III, which are free from inhibition by L-valine an can catalyze the conversion of: (a) pyruvate to &agr;-acetolactate and (b) &agr;-ketobutyrate and pyruvate to &agr;-aceto-a-hydroxybutyrate. The present invention also provides methods for producing L-valine by fermentation of a bacterium harboring the novel DNA molecule and/or expressing the mutant acetohydroxy acid synthase isozyme III.
摘要翻译： 本发明提供了编码源自大肠杆菌的乙酰羟酸合酶同功酶III的小亚基的分离的DNA分子，并且没有L-缬氨酸的抑制的大肠杆菌乙酰羟酸合酶同功酶III的突变体可以催化转化： a）丙酮酸至α-乙酰乳酸和（b）α-酮丁酸和丙酮酸至α-乙酰-α-羟基丁酸。 本发明还提供了通过发酵含有新的DNA分子的细菌和/或表达突变型乙酰羟酸合酶同功酶III来生产L-缬氨酸的方法。
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