利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.012 秒)

    Method for producing L-lysine
    7.
    发明授权
    Method for producing L-lysine 失效
    生产L-赖氨酸的方法

    公开(公告)号：US06221636B1

    公开(公告)日：2001-04-24

    申请号：US08985916

    申请日：1997-12-05

    申请人： Atsushi Hayakawa ,  Masakazu Sugimoto ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    发明人： Atsushi Hayakawa ,  Masakazu Sugimoto ,  Yasuhiko Yoshihara ,  Tsuyoshi Nakamatsu

    IPC分类号： C12P1308

    CPC分类号： C12N9/1217 ,  C12N9/88 ,  C12P13/08

    摘要： A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.

    摘要翻译： 在棒状杆菌细胞的细胞中自主复制的重组DNA，其中包含编码天冬氨酸激酶的DNA序列，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，以及编码二氨基庚二酸脱羧酶的DNA序列; 携带天冬氨酸激酶的棒状细菌，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，并且包含编码二氨基庚二酸脱羧酶的增强的DNA序列; 以及L-赖氨酸的制造方法，其特征在于，在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，从培养物中收集L-赖氨酸。

    Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum
    8.
    发明授权
    Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum 失效
    编码来自谷氨酸棒杆菌的组氨酸激酶的核苷酸序列

    公开(公告)号：US06746854B2

    公开(公告)日：2004-06-08

    申请号：US09824551

    申请日：2001-08-01

    申请人： Brigitte Bathe ,  Caroline Kreutzer ,  Achim Marx ,  Walter Pfefferle

    发明人： Brigitte Bathe ,  Caroline Kreutzer ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12P1308

    CPC分类号： C12N9/1223 ,  C12P13/08

    摘要： This invention relates to novel polynucleotide sequences encoding the histidine kinase luminescence expression sensor (luxS) gene from Corynebaclerium glutamicum, probes to the novel polynucleotide sequences encoding the luxS gene, vector and host cells containing the novel luxS polynucleotide sequences, the encoded Lux S polypeptide, and a process for the fermentative preparation of amino acids using bacteria which the luxS gene is attenuated.

    摘要翻译： 本发明涉及编码来自谷氨酸棒杆菌的组氨酸激酶发光表达传感器（luxS）基因的新型多核苷酸序列，探针编码luxS基因的新型多核苷酸序列，含有新型luxS多核苷酸序列的载体和宿主细胞，编码的Lux S多肽， 以及使用luxS基因减毒的细菌发酵制备氨基酸的方法。

    Nucleotide sequences which code for the zwa1 gene
    9.
    发明授权
    Nucleotide sequences which code for the zwa1 gene 失效
    编码zwa1基因的核苷酸序列

    公开(公告)号：US06632644B2

    公开(公告)日：2003-10-14

    申请号：US09731909

    申请日：2000-12-08

    申请人： Bettina Möckel ,  Walter Pfefferle ,  Achim Marx ,  Jörn Kalinowski ,  Brigitte Bathe ,  Alfred Pühler

    发明人： Bettina Möckel ,  Walter Pfefferle ,  Achim Marx ,  Jörn Kalinowski ,  Brigitte Bathe ,  Alfred Pühler

    IPC分类号： C12P1308

    CPC分类号： C07K14/34 ,  C12N9/00 ,  C12P13/08

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含氨基酸序列 b）编码多肽的多核苷酸，其包含与SEQ ID NO 2的氨基酸序列至少70％的程度相同的氨基酸序列，c） 与a）和b）和d）多核苷酸互补的多核苷酸，其包含a），b）或c），/或PTEXT的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及发酵 在使用棒状细菌中扩增zwa1基因的L-氨基酸的制备

    Process for producing L-amino acids
    10.
    发明授权
    Process for producing L-amino acids 失效
    生产L-氨基酸的方法

    公开(公告)号：US06319696B1

    公开(公告)日：2001-11-20

    申请号：US09011762

    申请日：1998-04-17

    申请人： Hiroko Kishino ,  Masako Izui ,  Yukiko Ono ,  Hisao Ito ,  Osamu Kurahashi

    发明人： Hiroko Kishino ,  Masako Izui ,  Yukiko Ono ,  Hisao Ito ,  Osamu Kurahashi

    IPC分类号： C12P1308

    CPC分类号： C12P13/04

    摘要： A microorganism, which has an ability to produce L-amino acid, especially L-phenylalanine, L-tryptophane, L-tyrosine, L-threonine, or L-isoleucine, in which a phosphoenolpyruvate-producing ability is enhanced, is cultivated in a medium so that the L-amino acid is produced and accumulated in the medium to collect the L-amino acid.

    摘要翻译： 具有生产L-氨基酸，特别是L-苯丙氨酸，L-色氨酸，L-酪氨酸，L-苏氨酸或L-异亮氨酸的能力的微生物，其中增加了产生磷酸烯醇丙酮酸的能力， 培养基中，使L-氨基酸产生并积累在培养基中以收集L-氨基酸。