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公开(公告)号：US06346379B1
公开(公告)日：2002-02-12
申请号：US09146631
申请日：1998-09-03
申请人： David Harrow Gelfand , Lisa Vivian Kalman , Fred Lawrence Reichert , Christopher Lim Sigua , Thomas W. Myers
发明人： David Harrow Gelfand , Lisa Vivian Kalman , Fred Lawrence Reichert , Christopher Lim Sigua , Thomas W. Myers
IPC分类号： C12Q168
CPC分类号： C12N9/1252
摘要： Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
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2.发明授权Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescent dyes 有权掺有荧光染料标记的核苷三磷酸的热稳定DNA聚合酶公开(公告)号：US07598032B2
公开(公告)日：2009-10-06
申请号：US10355532
申请日：2003-01-30
申请人： David Harrow Gelfand , Lisa Vivian Kalman , Fred Lawrence Reichert , Christopher Lim Sigua , Thomas W. Myers
发明人： David Harrow Gelfand , Lisa Vivian Kalman , Fred Lawrence Reichert , Christopher Lim Sigua , Thomas W. Myers
CPC分类号： C12N9/1252
摘要： Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
摘要翻译： 具有提高非常规核苷酸效率的修饰的耐热性DNA聚合酶，例如用荧光素家族染料标记的那些核苷酸，在许多体外DNA合成应用中是有利的。 这样的酶特别可用于链终止核酸测序方案，以及这些酶的天然形式。 编码修饰酶的基因及其生产和使用方法为DNA测序提供了成本和效率优势。
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3.发明授权Thermostable DNA polymerases having reduced discrimination against ribo-NTPs 失效热稳定的DNA聚合酶减少了对ribo-NTPs的歧视
公开(公告)号：US5939292A
公开(公告)日：1999-08-17
申请号：US906484
申请日：1997-08-05
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N15/10 , C12N15/54 , C12P21/02 , C12Q1/68 , C12R1/19 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6848 , C12N9/1252 , C12Q1/6869
摘要： Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides, such as ribonucleotides, into DNA products, are advantageous in many in vitro synthesis applications. Such enzymes are particularly useful for use in nucleic acid sequencing protocols and provide novel means for DNA sequence analysis. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
摘要翻译： 具有增强的非常规核苷酸（例如核糖核苷酸）掺入DNA产物的效率的修饰的热稳定DNA聚合酶在许多体外合成应用中是有利的。 这些酶特别可用于核酸测序方案，并为DNA序列分析提供新的手段。 编码修饰酶的基因及其生产和使用方法为DNA测序提供了成本和效率优势。
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