Alpha-amylase mutants
    1.
    发明授权
    Alpha-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号:US07566561B2

    公开(公告)日:2009-07-28

    申请号:US10327837

    申请日:2002-12-23

    IPC分类号: C12N9/28 C12N15/56

    摘要: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    摘要翻译: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度(相对于亲本)下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体在酸性pH和/或低Ca 2+浓度(相对于亲本)中表现出增加的热稳定性。

    Alpha-Amylase Mutants
    5.
    发明申请
    Alpha-Amylase Mutants 审中-公开
    α-淀粉酶突变体

    公开(公告)号:US20120283164A1

    公开(公告)日:2012-11-08

    申请号:US13556623

    申请日:2012-07-24

    IPC分类号: C12N9/26 C11D3/386

    摘要: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    摘要翻译: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度(相对于亲本)下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体在酸性pH和/或低Ca 2+浓度(相对于亲本)中表现出增加的热稳定性。

    &agr;-amylase mutants
    6.
    发明授权
    &agr;-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号:US06528298B1

    公开(公告)日:2003-03-04

    申请号:US09545586

    申请日:2000-04-07

    IPC分类号: C12P2106

    摘要: The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    摘要翻译: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度(相对于亲本)下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体在酸性pH和/或低Ca 2+浓度(相对于亲本)中表现出增加的热稳定性。

    &agr;-amylase and &agr;-amylase variants
    8.
    发明授权
    &agr;-amylase and &agr;-amylase variants 有权
    α-淀粉酶和α-淀粉酶变体

    公开(公告)号:US06361989B1

    公开(公告)日:2002-03-26

    申请号:US09290734

    申请日:1999-04-13

    IPC分类号: C12N900

    摘要: The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    摘要翻译: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度(相对于亲本)下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体在酸性pH和/或低Ca 2+浓度(相对于亲本)中表现出增加的热稳定性。

    Alpha-amylase mutants
    9.
    发明授权
    Alpha-amylase mutants 有权
    α-淀粉酶突变体

    公开(公告)号:US08263368B2

    公开(公告)日:2012-09-11

    申请号:US12493860

    申请日:2009-06-29

    IPC分类号: C12N9/28 C12S11/00 C12P19/14

    摘要: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

    摘要翻译: 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度(相对于亲本)下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体,携带本发明的DNA构建体的重组表达载体,用本发明的DNA构建体转化的细胞,使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤,纺织品退浆,淀粉液化,包含本发明的α-淀粉酶变体的洗涤剂添加剂,手动或自动洗碗洗涤剂组合物,其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法,该变体在酸性pH和/或低Ca 2+浓度(相对于亲本)中表现出增加的热稳定性。