公开(公告)号：US07829329B2

公开(公告)日：2010-11-09

申请号：US10306275

申请日：2002-11-27

申请人： Terry Hermiston ,  Lynda K. Hawkins ,  Leisa Johnson

发明人： Terry Hermiston ,  Lynda K. Hawkins ,  Leisa Johnson

IPC分类号： C12N15/00 ,  C12N7/00 ,  A01N43/04

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K38/1761 ,  A61K38/191 ,  A61K38/195 ,  A61K38/20 ,  A61K38/217 ,  A61K48/00 ,  C12N2710/10332 ,  C12N2710/10343

摘要： Adenoviral vectors, including mutant adenoviruses, that have restriction sites in the E3 region, that facilitate its partial or total deletion, or select genes contained therein, and optionally compositions and methods for substituting heterologous gene(s) in the partially or totally deleted E3 region(s), which heterologous gene(s) being operably linked to endogenous adenoviral transcriptional control sequences will exhibit an expression pattern, both in terms of timing and degree of expression, similar to the endogenous adenoviral gene(s) that it replaces, and further optionally including mutations in other parts of the adenoviral genome, including certain E1B or E1A regions, and that have applications for diagnosing or treating disease, preferably disease involving unwanted cell growth, including cancer.

摘要翻译： 在E3区域具有限制性位点，促进其部分或全部缺失的腺病毒载体，或其中所含的选择基因的任何组合物和方法，以及在部分或全部缺失的E3区域中取代异源基因的组合物和方法 其异源基因与内源性腺病毒转录控制序列可操作地连接将在时间和表达程度方面表现出类似于其所取代的内源性腺病毒基因的表达模式，并且进一步 任选地包括在腺病毒基因组的其他部分中的突变，包括某些E1B或E1A区域，并且具有用于诊断或治疗疾病，优选涉及不想要的细胞生长的疾病（包括癌症）的应用。