公开(公告)号：US20060094005A1

公开(公告)日：2006-05-04

申请号：US10332134

申请日：2001-07-06

申请人： Helen Lee ,  Magda Dineva ,  Shaun Hazlewood

发明人： Helen Lee ,  Magda Dineva ,  Shaun Hazlewood

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6834 ,  G01N30/02 ,  G01N30/94 ,  C12Q2565/625 ,  C12Q2537/162 ,  B01D15/3804

摘要： Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is connected with the contact end of the dipstick to cause the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.

摘要翻译： 描述了在试纸测定中使用辅助探针。 在用于测试样品溶液中目标核酸的存在的试纸测试中，样品溶液与量油尺的接触端连接，以使样品溶液与量油尺的接触端接触以引起样品溶液 通过毛细管作用移动到捕获靶核酸的测试棒的捕获区域。 可以通过能够与靶核酸杂交的捕获探针在捕获区捕获靶核酸。 能够与靶核酸杂交的标记检测探针可用于检测捕获区的靶核酸。 可以使用辅助探针来增强捕获和/或检测探针与靶核酸的结合，从而提高靶核酸检测的灵敏度。 还描述了骰子和工具包。

公开(公告)号：US20070190548A1

公开(公告)日：2007-08-16

申请号：US11624861

申请日：2007-01-19

申请人： Helen LEE ,  Magda Dineva ,  Shaun Hazlewood

发明人： Helen LEE ,  Magda Dineva ,  Shaun Hazlewood

IPC分类号： C12Q1/68 ,  C12M3/00 ,  C07H21/04

CPC分类号： C12Q1/6834 ,  G01N30/02 ,  G01N30/94 ,  C12Q2565/625 ,  C12Q2537/162 ,  B01D15/3804

摘要： Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.

摘要翻译： 描述了在试纸测定中使用辅助探针。 在用于测试样品溶液中目标核酸的存在的试纸测试中，将样品溶液与量油尺的接触端接触，以使样品溶液通过毛细作用移动到量油尺的捕获区， 捕获靶核酸。 可以通过能够与靶核酸杂交的捕获探针在捕获区捕获靶核酸。 能够与靶核酸杂交的标记检测探针可用于检测捕获区的靶核酸。 可以使用辅助探针来增强捕获和/或检测探针与靶核酸的结合，从而提高靶核酸检测的灵敏度。 还描述了骰子和工具包。