公开(公告)号：US20070190548A1

公开(公告)日：2007-08-16

申请号：US11624861

申请日：2007-01-19

申请人： Helen LEE ,  Magda Dineva ,  Shaun Hazlewood

发明人： Helen LEE ,  Magda Dineva ,  Shaun Hazlewood

IPC分类号： C12Q1/68 ,  C12M3/00 ,  C07H21/04

CPC分类号： C12Q1/6834 ,  G01N30/02 ,  G01N30/94 ,  C12Q2565/625 ,  C12Q2537/162 ,  B01D15/3804

摘要： Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.

摘要翻译： 描述了在试纸测定中使用辅助探针。 在用于测试样品溶液中目标核酸的存在的试纸测试中，将样品溶液与量油尺的接触端接触，以使样品溶液通过毛细作用移动到量油尺的捕获区， 捕获靶核酸。 可以通过能够与靶核酸杂交的捕获探针在捕获区捕获靶核酸。 能够与靶核酸杂交的标记检测探针可用于检测捕获区的靶核酸。 可以使用辅助探针来增强捕获和/或检测探针与靶核酸的结合，从而提高靶核酸检测的灵敏度。 还描述了骰子和工具包。

公开(公告)号：US20080160516A1

公开(公告)日：2008-07-03

申请号：US11686580

申请日：2007-03-15

申请人： Helen LEE ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

发明人： Helen LEE ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6834 ,  C12Q1/689 ,  C12Q2565/625

摘要： Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.

摘要翻译： 描述了用于测试样品溶液中目标核酸存在的浸渍条，浸渍条包括固定在量油尺的捕获区的通用捕获探针。 通用捕获探针能够与钩状捕获探针杂交，其与样品溶液中的靶核酸杂交。 将量油尺的接触端与样品溶液接触，使钩捕捉探针与目标核酸杂交，通过毛细管作用移动到可以检测靶核酸的捕获区。 使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子，从而简化骰子的制备。 然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。 还描述了方法和试剂盒。