公开(公告)号：US5601757A

公开(公告)日：1997-02-11

申请号：US517008

申请日：1995-08-18

申请人： Manfred Biselli ,  Christoph Born ,  Bernd Scr oder ,  Christian Wandrey

发明人： Manfred Biselli ,  Christoph Born ,  Bernd Scr oder ,  Christian Wandrey

IPC分类号： C12M1/04 ,  C12M1/12 ,  C12M1/40 ,  C12M3/00 ,  C12M3/06 ,  C10J1/08

CPC分类号： C12M25/20 ,  C12M23/40 ,  C12M29/04 ,  Y10S435/818

摘要： A cell culture reactor can have one or more gasification modules in which tubular membrane hoses surround rigid inner tubes which extend above the membranes to communicate with gas inlets or outlets. The tube is anchored in a closure member at the lower end of the membrane.

摘要翻译： 细胞培养反应器可以具有一个或多个气化模块，其中管状膜软管包围在膜上方延伸以与气体入口或出口连通的刚性内管。 管被锚定在膜的下端的封闭构件中。

公开(公告)号：US5906940A

公开(公告)日：1999-05-25

申请号：US602137

申请日：1996-02-15

申请人： Christian Wandrey ,  Manfred Biselli ,  Bernd Schroder ,  Hans-Joachim Schmoll

发明人： Christian Wandrey ,  Manfred Biselli ,  Bernd Schroder ,  Hans-Joachim Schmoll

IPC分类号： C12M1/04 ,  C12M1/40 ,  C12M3/06 ,  C12N5/06 ,  C12N5/08 ,  C12N11/02 ,  C12N11/14

CPC分类号： C12M25/20 ,  C12M25/14

摘要： Stem cells or other organ-function cells are cultivated in a fluidized bed system on macroporous glass carrier bodies treated with a structure protein such as gelatin and an extracellular matrix protein such as fibronectin, and coated with a stroma cell layer. Glass carriers coated with gelatin are added to a fluidized bed reactor, and a culture medium containing an extracellular matrix protein is added to bind the protein to the gelatin. Stromal cells are then added and the cells are cultured to immobilize the cells on the carriers containing the bound protein. Immature organ-function cells are added to the reactor, and while generating a fluidized bed of the carriers in the culture medium, the culture medium is recirculated from and to the reactor in a recirculation loop. Bubble-free aeration of the culture medium is effected to cultivate the immature organ-function cells on the carriers to obtain both mature differentiated organ-function cells and progenitor organ-function cells. Mature differentiated organ-function cells which are released from the carriers are harvested from the reactor while retaining the progenitor organ-function cells which adhere to the carriers to provide an equilibrium between the harvested mature differentiated organ-function cells, the progenitor cells and the immature cells added to the reactor. Growth and regulatory factors for cultivating organ-function cells can be produced by culturing immobilized stromal cells in the reactor in the absence of the organ-function cells.

摘要翻译： 干细胞或其他器官功能细胞在用结构蛋白如明胶和细胞外基质蛋白如纤连蛋白处理的大孔玻璃载体上在流化床系统中培养，并涂覆有基质细胞层。 将涂有明胶的玻璃载体加入到流化床反应器中，并加入含有细胞外基质蛋白的培养基以将蛋白质与明胶结合。 然后加入基质细胞，并培养细胞以将细胞固定在含有结合蛋白的载体上。 将未成熟的器官功能细胞加入到反应器中，并且在培养基中产生载体的流化床的同时，培养基在循环回路中从反应器再循环。 培养培养基的无气泡通气培养载体上的未成熟器官功能细胞以获得成熟分化的器官功能细胞和祖细胞器官功能细胞。 从反应器中收集从载体释放的成熟的分化器官功能细胞，同时保留粘附于载体的祖细胞器官功能细胞，以在收获的成熟分化的器官功能细胞，祖细胞和未成熟细胞之间提供平衡 将细胞加入到反应器中。 培养器官功能细胞的生长和调节因子可以通过在不存在器官功能细胞的情况下在反应器中培养固定的基质细胞来产生。

公开(公告)号：US5130026A

公开(公告)日：1992-07-14

申请号：US705655

申请日：1991-05-24

申请人： Manfred Biselli ,  Joerg Thoemmes ,  Christian Wandrey

发明人： Manfred Biselli ,  Joerg Thoemmes ,  Christian Wandrey

IPC分类号： B01D15/04 ,  B01D61/24 ,  B01J47/12 ,  C01C1/02 ,  C02F1/469 ,  C02F1/58 ,  C12M1/12 ,  C12M3/06

CPC分类号： B01D61/243 ,  C12M29/04 ,  C12M41/26 ,  C12M47/10

摘要： A process for the removal of ammonium from aqueous liquids, especially from fermentation broths, is disclosed. The process uses a cation exchanger membrane which separates a liquid on a donor side that is to be depleted of NH.sub.4.sup.+ from a solution on an acceptor side whose pH is higher than on the donor side. Because of the equilibriumNH.sub.4.sup.+ .revreaction.NH.sub.3 +H+there is on the acceptor side increased formation of NH.sub.3. This is continuously removed, in particular by reducing the pressure and/or passing through inert gas, or by selective transport through another microporous membrane into an acid protonation solution, so that a NH.sub.4.sup.+ concentration gradient is maintained across the membrane. A similar content, apart from the ammonium concentration, of concomitant cations on the donor and acceptor side and/or a high ratio of the solution volumes on the donor side and the acceptor side ensures that the solution on the donor side to be depleted of NH.sub.4.sup.+, does not lose concomitant ions. A suitable apparatus comprises a flow section which can be coupled into a bypass and has a cation exchanger limiting membrane which is simultaneously a limiting wall of an acceptor chamber. The acceptor chamber may contain devices for pH control and NH.sub.3 removal or these may be contained in a flow section.

摘要翻译： 公开了从含水液体，特别是发酵液中除去铵的方法。 该方法使用阳离子交换膜，其将待排出NH 4 +的供体侧上的液体从pH高于供体侧的受体侧上的溶液分离。 由于平衡NH4 + - > < - NH3 + H +在受体侧上增加了NH3的形成。 这是连续去除的，特别是通过降低压力和/或通过惰性气体，或通过选择性地将另一个微孔膜输送到酸性质子溶液中，使得横跨该膜保持NH 4 +浓度梯度。 供体和受体侧上伴随的阳离子和/或供体侧和受体侧的溶液体积的高比例，除了铵浓度以外，类似的含量确保供体侧的溶液被耗尽NH4 + ，不会伴随离子丢失。 合适的装置包括流动部分，其可以耦合到旁路中并且具有同时是受体室的限制壁的阳离子交换器限制膜。 受体室可以包含用于pH控制和NH 3去除的装置，或者这些装置可以包含在流动部分中。