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    Methods and devices for the long-term culture of hematopoietic progenitor cells
    1.
    发明授权
    Methods and devices for the long-term culture of hematopoietic progenitor cells 失效
    造血祖细胞长期培养的方法和装置

    公开(公告)号：US06645489B2

    公开(公告)日：2003-11-11

    申请号：US10143540

    申请日：2002-05-10

    申请人： Mark J. Pykett ,  Michael Rosenzweig ,  Richard B. Kaplan

    发明人： Mark J. Pykett ,  Michael Rosenzweig ,  Richard B. Kaplan

    IPC分类号： C12N508

    CPC分类号： C12M23/20 ,  A61K2035/124 ,  C12M25/14 ,  C12M29/10 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2510/00 ,  C12N2533/30 ,  C12N2533/52

    摘要： The invention pertains to methods and devices for the long term, in vitroculture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

    摘要翻译： 本发明涉及在没有外源性添加的造血生长因子的情况下在造血祖细胞的培养中长期的方法和装置，用于将外来遗传物质引入造血源的细胞的改进方法以及用于进行这些方法的装置 。 造血祖细胞在三维多孔生物材料上培养。 三维多孔生物材料增强造血祖细胞的存活并导致祖细胞数量和/或功能的扩大，同时在没有外源生长因子的情况下保持祖细胞多能性。 此外，三维多孔生物材料支持在这种环境下培养的细胞上的高水平转导。

    Methods and devices for the long-term culture of hematopoietic progenitor cells
    2.
    发明授权
    Methods and devices for the long-term culture of hematopoietic progenitor cells 失效
    造血祖细胞长期培养的方法和装置

    公开(公告)号：US06440734B1

    公开(公告)日：2002-08-27

    申请号：US09509379

    申请日：2000-06-07

    申请人： Mark J. Pykett ,  Michael Rosenzweig ,  Richard B. Kaplan

    发明人： Mark J. Pykett ,  Michael Rosenzweig ,  Richard B. Kaplan

    IPC分类号： C12N502

    CPC分类号： C12M23/20 ,  A61K2035/124 ,  C12M25/14 ,  C12M29/10 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2510/00 ,  C12N2533/30 ,  C12N2533/52

    摘要： The invention pertains to methods and devices for the long term, in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

    摘要翻译： 本发明涉及在没有外源性添加的造血生长因子的情况下造血祖细胞的长期体外培养的方法和装置，用于将外源遗传物质引入造血来源的细胞的改进方法，以及用于进行这些 方法。 造血祖细胞在三维多孔生物材料上培养。 三维多孔生物材料增强造血祖细胞的存活并导致祖细胞数量和/或功能的扩大，同时在没有外源生长因子的情况下保持祖细胞多能性。 此外，三维多孔生物材料支持在这种环境下培养的细胞上的高水平转导。