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公开(公告)号:US20130022500A1
公开(公告)日:2013-01-24
申请号:US13554370
申请日:2012-07-20
摘要: A clamping insert for cell culture includes: a lower support having: i. a hollow member sized to be engaged in a well of a microplate, the hollow member being open at both its ends and including a support surface at one of its ends, ii. first tightening elements, an inner holder sized to be fitted inside the hollow member, and including second tightening elements arranged to cooperate with the first tightening elements of the hollow member to tighten reversibly between the inner holder and the support surface of the lower support a porous substrate, the inner holder including an opened channel keeping free the porous substrate; retaining elements intended to cooperate with the well of the microplate to allow the clamping insert to be suspended in the well, sealing elements to hermetically seal the contact area between the clamping insert and the porous substrate.
摘要翻译: 用于细胞培养的夹持插入物包括:下支撑体,其具有:i。 尺寸适于接合在微孔板的孔中的中空构件,所述中空构件在其两端开放并且在其一端处包括支撑表面,ii。 第一紧固元件,尺寸适于安装在中空构件内部的内部保持器,并且包括第二紧固元件,其布置成与中空构件的第一紧固元件协作以在内部保持器和下部支撑件的支撑表面之间可逆地收紧多孔 所述内保持器包括保持多孔基底的开放通道; 保持元件旨在与微板的孔配合以允许夹持插入件悬挂在井中,密封元件以气密地密封夹紧插入件和多孔基底之间的接触区域。
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公开(公告)号:US5763191A
公开(公告)日:1998-06-09
申请号:US279715
申请日:1994-07-25
申请人: Wolfgang Knoll , Franz-Josef Schmitt , Christian Klein , Hans-Joachim Guder , Martha Liley , Jurgen Spinke
发明人: Wolfgang Knoll , Franz-Josef Schmitt , Christian Klein , Hans-Joachim Guder , Martha Liley , Jurgen Spinke
IPC分类号: G01N33/543 , G01N33/553 , G01N33/53 , B05D3/10 , B32B15/00
CPC分类号: B82Y15/00 , B82Y30/00 , B82Y40/00 , G01N33/54353 , G01N33/553 , Y10T428/12493 , Y10T428/12535 , Y10T428/12556
摘要: The invention concerns a binding matrix containing a carrier material and a solid phase reactant which is adsorbed to this via anchor groups that is capable of binding to at least one free reaction partner wherein the solid phase reactant forms a dilute and essentially laterally homogeneous binding layer on the surface of the carrier material. In addition a method for the determination of an analyte in a sample solution is claimed in which a solid phase reactant is used which is a component of a binding matrix according to the present invention. In this process the specific binding reaction is preferably determined by optical reflection techniques.
摘要翻译: 本发明涉及含有载体材料和固相反应物的结合基质,其通过能够结合至少一个游离反应配偶体的锚定基团吸附到其上,其中固相反应物形成稀释且基本上横向均匀的结合层 载体材料的表面。 此外,还要求一种用于测定样品溶液中的分析物的方法,其中使用作为根据本发明的结合基质的组分的固相反应物。 在该方法中,特异性结合反应优选通过光学反射技术来确定。
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公开(公告)号:US09371508B2
公开(公告)日:2016-06-21
申请号:US13554370
申请日:2012-07-20
摘要: A clamping insert for cell culture having a lower support with a hollow member engagable in a well of a microplate, the hollow member being open at both ends and including a support surface at one end, and first tightening elements. The clamping insert further having an inner holder sized to be fitted inside the hollow member, and second tightening elements arranged to cooperate with the first tightening elements of the hollow member to tighten reversibly between the inner holder and the support surface of the lower support a porous substrate. The inner holder further including an open channel keeping free the porous substrate. Retaining elements intended to cooperate with the well of the microplate are provided to allow the clamping insert to be suspended in the well, and sealing elements are provided to hermetically seal the contact area between the clamping insert and the porous substrate.
摘要翻译: 一种用于细胞培养的夹紧插入件,具有下部支撑件,其具有可接合在微孔板的孔中的中空构件,所述中空构件在两端敞开并且在一端包括支撑表面以及第一紧固元件。 所述夹紧插入件还具有尺寸适于装配在所述中空构件内部的内部保持器,以及第二紧固元件,所述第二紧固元件布置成与所述中空构件的所述第一紧固元件协作以在所述内部支架和所述下部支撑件的支撑表面之间可逆地收紧多孔 基质。 内部支架还包括保持空气的开放通道。 旨在与微孔板的孔配合的保持元件被设置成允许夹持插入件悬挂在井中,并且提供密封元件以气密地密封夹持插入件和多孔基底之间的接触区域。
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