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    Assay for nucleotide triphosphate using a modified nucleoside diphosphate kinase comprising a fluorescent label
    2.
    发明授权
    Assay for nucleotide triphosphate using a modified nucleoside diphosphate kinase comprising a fluorescent label 有权
    使用包含荧光标记的修饰的核苷二磷酸激酶测定核苷酸三磷酸

    公开(公告)号：US07297506B2

    公开(公告)日：2007-11-20

    申请号：US10779718

    申请日：2004-02-18

    申请人： Martin Hermann Klemens Brune ,  John Edgar Thomas Corrie ,  Martin Ronald Webb

    发明人： Martin Hermann Klemens Brune ,  John Edgar Thomas Corrie ,  Martin Ronald Webb

    IPC分类号： C12Q1/48 ,  G01N33/53 ,  C12N9/12

    CPC分类号： C12Q1/48 ,  C12N9/12

    摘要： Nucleoside diphosphates (NDPs) are detected or measured by following the dephosphorylation of the phosphoenzyme form of nucleoside diphosphate kinase (NDPK), and nucleoside triphosphates (NTPs) are detected or measured by following the phosphorylation of NDPK to its phosphoenozyme form. A typical process involves (a) causing NDP in sample to bind to NDPK phosphoenzyme, or causing NTP in sample to phosphorylate NDPK and (b) detecting a change in a characteristic of the enzyme which differs between its phosphorylated and unphosphorylated forms. This may be aided by labelling the enzyme. Quantitative data can be obtained. Both in vivo and in vitro measurements can be made.

    摘要翻译： 通过跟踪磷酸酶形式的核苷二磷酸激酶（NDPK）的去磷酸化来检测或测量核苷二磷酸（NDP），并通过遵循NDPK磷酸化为磷酸酶形式来检测或测量核苷三磷酸（NTPs）。 典型的方法包括：（a）使样品中的NDP与NDPK磷酸酶结合，或使样品中的NTP磷酸化NDPK，和（b）检测其磷酸化和非磷酸化形式之间不同的酶的特征变化。 这可以通过标记酶来辅助。 可以获得定量数据。 可以进行体内和体外测量。

    Mineral winning ploughs
    4.
    发明授权
    Mineral winning ploughs 失效
    矿物犁

    公开(公告)号：US4035025A

    公开(公告)日：1977-07-12

    申请号：US646102

    申请日：1976-01-02

    申请人： Martin Hermann ,  Oswald Breuer ,  Alois Hauschopp

    发明人： Martin Hermann ,  Oswald Breuer ,  Alois Hauschopp

    IPC分类号： E21C27/32 ,  E21C27/34 ,  E21C27/35

    CPC分类号： E21C27/32 ,  E21C27/34

    摘要： A coal plough is composed of two spaced-apart interconnected bodies which engage on a guide and which are moved along the guide to strip coal from a coal face. Each body is provided with various sets of cutter bits located at both its end regions and a floor cutter adjustable to control the height of cutting. The cutter bits at the inner face end regions of the bodies project further outwards towards the coal face than the other bits. During operation when the bodies move in one direction along the guide, cutter bits at the forward outer end region of the leading body cut into the coal face and the following-up cutter bits at the forward inner end region of the lagging body also cut into the face. The remaining cutter bits do not engage on the coal face.In this way the cutting operation is performed by both bodies and similarly both bodies can assist into transferring the coal detached from the face over the guide and into a scraper-chain conveyor.

    摘要翻译： 煤犁由两个间隔开的相互连接的主体组成，这两个相互连接的主体接合在引导件上并且沿导向件移动以从煤面剥离煤。 每个主体都设置有位于其两个端部区域的各种刀具位置，并且地板刀具可调节以控制切割高度。 尸体的内表面末端区域的切削刀头比其他钻头更向外朝向煤面突出。 在主体沿着导向件沿一个方向移动的操作过程中，前导体前端区域的切削刀头切入煤面，并且滞后体的前端内部区域的后刀切削刀头也切入 的脸。 剩余的刀头不接合在煤面上。

    REPLACEABLE TRIMMER LINE DEVICE
    7.
    发明申请
    REPLACEABLE TRIMMER LINE DEVICE 审中-公开
    可更换的TRIMMER线设备

    公开(公告)号：US20160044864A1

    公开(公告)日：2016-02-18

    申请号：US14457254

    申请日：2014-08-12

    申请人： Martin Hermann

    发明人： Martin Hermann

    IPC分类号： A01D34/416

    CPC分类号： A01D34/4166 ,  A01D34/4168

    摘要： A replaceable trimmer line device facilitates changing of trimmer line extending from a trimmer head. The device includes a line connector having opposite ends coupled to a trimmer head. A line socket extends through the line connector. A trimmer line has a head section and a cutting section. The cutting section extends from the head section. The head section is positionable in the socket such that the cutting section extends laterally from the line connector and outwardly from the trimmer head.

    摘要翻译： 可更换的修剪器线装置有助于改变从修剪头延伸的修剪线。 该装置包括具有联接到修剪器头的相对端的线连接器。 线插座延伸穿过线路连接器。 修剪线具有头部和切割部。 切割部分从头部延伸。 头部可定位在插座中，使得切割部分从线连接器横向延伸并从修剪器头部向外延伸。

    Assay for nucleoside diphosphate using a modified nucleoside diphosphate kinase comprising a fluorescent label
    9.
    发明授权
    Assay for nucleoside diphosphate using a modified nucleoside diphosphate kinase comprising a fluorescent label 有权
    使用包含荧光标记的修饰的核苷二磷酸激酶测定核苷二磷酸

    公开(公告)号：US06746849B1

    公开(公告)日：2004-06-08

    申请号：US09937296

    申请日：2001-11-14

    申请人： Martin Hermann Klemens Brune ,  John Edgar Thomas Corrie ,  Martin Ronald Webb

    发明人： Martin Hermann Klemens Brune ,  John Edgar Thomas Corrie ,  Martin Ronald Webb

    IPC分类号： C12Q148

    CPC分类号： C12Q1/48 ,  C12N9/12

    摘要： Nucleoside diphosphates (NDPs) are detected or measured by following the dephosphorylation of the phosphoenzyme form of nucleoside diphosphate kinase (NDPK), and nucleoside triphosphates (NTPs) are detected or measured by following the phosphorylation of NDPK to its phosphoenzyme form. A typical process involves (a) causing NDP in sample to bind to NDPK phosphoenzyme, or causing NTP in sample to phosphorylate NDPK and (b) detecting a change in a characteristic of the enzyme which differs between its phosphorylated and unphosphorylated forms. This may be aided by labelling the enzyme. Quantitative data can be obtained. Both in vivo and in vitro measurements can be made.

    摘要翻译： 通过跟踪磷酸酶形式的核苷二磷酸激酶（NDPK）的去磷酸化来检测或测量核苷二磷酸（NDP），并且通过遵循NDPK磷酸化至其磷酸酶形式来检测或测量核苷三磷酸（NTPs）。 典型的方法包括：（a）使样品中的NDP与NDPK磷酸酶结合，或使样品中的NTP磷酸化NDPK，和（b）检测其磷酸化和非磷酸化形式之间不同的酶的特征变化。 这可以通过标记酶来辅助。 可以获得定量数据。 可以进行体内和体外测量。