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公开(公告)号：US08658392B2
公开(公告)日：2014-02-25
申请号：US13508436
申请日：2010-10-26
IPC分类号： C12Q1/04
CPC分类号： G01N21/77 , G01N21/6408 , G01N21/6486 , G01N2021/7786
摘要： The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.
摘要翻译： 本发明提供一种通过使用藻类来评价化学品的毒性的方法，其特征在于，包括：（a）通过加热来解冻冷冻藻类细胞的解冻步骤，并通过向其中加入培养基稀释所得细胞的悬浮液; （b）在解冻步骤（a）中培养获得的藻细胞以使藻类细胞从冷冻和解冻的作用中恢复的回收培养步骤; （c）在回收培养步骤（b）之后收集部分藻类细胞的确认步骤，通过向其中加入培养基稀释该部分藻类细胞，并测定其中的延迟发光的发光量 藻细胞作为初始值数据; （d）在确认步骤（c）之后将藻类细胞与含有测试物质的溶液混合以制备曝光样品并培养曝光样品的曝光步骤; （e）在曝光步骤（d）之后测量曝光样品的延迟发光的发光量作为曝光数据的测量步骤; 以及（f）基于初始值数据和曝光数据计算评价值，并根据评价值来评价被检物质的毒性的评价步骤。
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公开(公告)号：US20120231491A1
公开(公告)日：2012-09-13
申请号：US13508436
申请日：2010-10-26
IPC分类号： G01N21/76
CPC分类号： G01N21/77 , G01N21/6408 , G01N21/6486 , G01N2021/7786
摘要： The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.
摘要翻译： 本发明提供一种通过使用藻类来评价化学品的毒性的方法，其特征在于，包括：（a）通过加热来解冻冷冻藻类细胞的解冻步骤，并通过向其中加入培养基稀释所得细胞的悬浮液; （b）在解冻步骤（a）中培养获得的藻细胞以使藻类细胞从冷冻和解冻的作用中恢复的回收培养步骤; （c）在回收培养步骤（b）之后收集部分藻类细胞的确认步骤，通过向其中加入培养基稀释该部分藻类细胞，并测定其中的延迟发光的发光量 藻细胞作为初始值数据; （d）在确认步骤（c）之后将藻类细胞与含有测试物质的溶液混合以制备曝光样品并培养曝光样品的曝光步骤; （e）在曝光步骤（d）之后测量曝光样品的延迟发光的发光量作为曝光数据的测量步骤; 以及（f）基于初始值数据和曝光数据计算评价值，并根据评价值来评价被检物质的毒性的评价步骤。
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3.发明授权公开(公告)号：US09759639B2
公开(公告)日：2017-09-12
申请号：US13821326
申请日：2011-07-14
CPC分类号： G01N1/30 , C12N1/04 , C12N1/12 , C12Q1/025 , G01N2333/405
摘要： The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase.
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4.发明授权公开(公告)号：US09448170B2
公开(公告)日：2016-09-20
申请号：US10583128
申请日：2004-12-16
CPC分类号： G01N21/6408 , G01N21/6486 , G01N2021/635
摘要： A biological growth inhibition factor assay method includes: a first step of mixing a photosynthetic sample, with an aqueous solution sample to prepare a test measurement solution, letting the test measurement solution stand, and then after illuminating light onto the test measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; a second step of mixing the photosynthetic sample with a standard sample, in which biological growth inhibition factors are not present, to prepare a standard measurement solution, letting the standard measurement solution stand, and then after illuminating light onto the standard measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; and a third step of computing assay values based on the light amounts of delayed fluorescence, respectively measured in the first step and the second step, and determining a comparison value of the assay values to assay biological growth inhibition factors. A biological growth inhibition factor assay method that enables analysis of a wide range of inhibition factors in a short time is thereby realized.
摘要翻译： 生物生长抑制因子测定方法包括：将光合作用样品与水溶液样品混合以制备测试测量溶液的第一步骤，使测试测量溶液静置，然后将光照射到测试测量溶液上以达到预定的 照射时间，测量发射的延迟荧光的光量; 将光合作用样品与其中不存在生物生长抑制因子的标准样品混合以制备标准测量溶液，使标准测量溶液静置，然后将光照射到标准测量溶液上以达到预定的第二步骤 照射时间，测量发射的延迟荧光的光量; 以及分别基于第一步骤和第二步骤中测量的延迟荧光的光量来计算测定值的第三步骤，以及测定测定值以测定生物生长抑制因子的比较值。 从而实现了能够在短时间内分析广泛的抑制因子的生物生长抑制因子测定方法。
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公开(公告)号：US06724984B2
公开(公告)日：2004-04-20
申请号：US10001998
申请日：2001-12-05
申请人： Yuji Kakuya , Keiji Ito , Hironori Mitsui , Koji Yamane , Tomofumi Horio , Masakazu Katsumata
发明人： Yuji Kakuya , Keiji Ito , Hironori Mitsui , Koji Yamane , Tomofumi Horio , Masakazu Katsumata
IPC分类号： H05B300
CPC分类号： A45D20/20 , A45D20/36 , A45D20/40 , A45D2020/245 , H05B3/0033 , H05B2203/032
摘要： A hair treatment promoting apparatus in which a reflector provided with a heater for radiating infrared rays is rotated by a motor so that the infrared rays are radiated to the top, back and both sides of a head of a subject. The heater and reflecting plate form a heating device rotated by the motor and a disk is provided which rotates with the motor. The disk is provided with a slit for a home position and slits for stopping arranged on both sides thereof, and energization of the motor is interrupted when the slit for the home position is detected within a prescribed time elapsed from when the slit for stopping is detected in the direction of rotating the heating device, thereby stopping the heating device at the home position.
摘要翻译： 一种头发处理促进装置，其中设置有用于辐射红外线的加热器的反射器由电动机旋转，使得红外线照射到被摄体的头部的顶部，后部和两侧。 加热器和反射板形成由电动机旋转的加热装置，并且设置有与电动机一起旋转的盘。 该盘具有用于起始位置的狭缝和布置在其两侧的用于停止的狭缝，并且当从检测到用于停止的狭缝的经过的规定时间内检测到用于原始位置的狭缝时，马达的通电被中断 在使加热装置旋转的方向上，从而将加热装置停止在起始位置。
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公开(公告)号：US08798931B2
公开(公告)日：2014-08-05
申请号：US12279789
申请日：2007-02-20
CPC分类号： G01N21/6486 , G01N21/6408 , G01N2021/635
摘要： An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample.An evaluation method for photosynthetic sample according to the present invention is for evaluating a state of a photosynthetic sample with a photosynthetic function based on temporal data of a luminescence amount of delayed luminescence emitted from the photosynthetic sample. First, characteristic values that indicate characteristics are determined for a plurality of time periods in the temporal data. An evaluation value is then computed by weighting the characteristic values. The state of the photosynthetic sample is then evaluated based on the evaluation value.
摘要翻译： 本发明的目的是适当且容易地评估评价样品中所含的光合作用样品的光合作用。 根据本发明的光合作用样品的评价方法是基于从光合作用样品发出的延迟发光的发光量的时间数据来评价具有光合作用功能的光合作用样品的状态。 首先，在时间数据中为多个时间段确定指示特性的特征值。 然后通过加权特征值来计算评估值。 然后根据评价值评价光合作用样品的状态。
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7.发明申请METHOD FOR PREPARATION OF ALGAL CELLS, AND KIT FOR EVALUATION OF TOXICITY OF CHEMICAL SUBSTANCE 有权制备ALGAL细胞的方法和用于评估化学物质毒性的试剂盒公开(公告)号：US20130177967A1
公开(公告)日：2013-07-11
申请号：US13821326
申请日：2011-07-14
IPC分类号： G01N1/30
CPC分类号： G01N1/30 , C12N1/04 , C12N1/12 , C12Q1/025 , G01N2333/405
摘要： The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase.
摘要翻译： 本发明提供一种制备用于通过延迟发光评价化学物质的毒性的藻类细胞的方法，该方法包括以对数生长期冷冻藻类细胞的步骤。
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8.发明申请EVALUATION METHOD FOR PHOTOSYNTHESIS SAMPLE, EVALUATION SYSTEM FOR PHOTOSYNTHESIS SAMPLE, AND EVALUATION PROGRAM FOR PHOTOSYNTHESIS SAMPLE 有权光合作用样品的评价方法，光合作用样品的评价系统和光合作用样品的评价程序公开(公告)号：US20100159496A1
公开(公告)日：2010-06-24
申请号：US12279789
申请日：2007-02-20
IPC分类号： C12Q1/02
CPC分类号： G01N21/6486 , G01N21/6408 , G01N2021/635
摘要： An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample.An evaluation method for photosynthetic sample according to the present invention is for evaluating a state of a photosynthetic sample with a photosynthetic function based on temporal data of a luminescence amount of delayed luminescence emitted from the photosynthetic sample. First, characteristic values that indicate characteristics are determined for a plurality of time periods in the temporal data. An evaluation value is then computed by weighting the characteristic values. The state of the photosynthetic sample is then evaluated based on the evaluation value.
摘要翻译： 本发明的目的是适当且容易地评估评价样品中所含的光合作用样品的光合作用。 根据本发明的光合作用样品的评价方法是基于从光合作用样品发出的延迟发光的发光量的时间数据来评价具有光合作用功能的光合作用样品的状态。 首先，在时间数据中为多个时间段确定指示特性的特征值。 然后通过加权特征值来计算评估值。 然后根据评价值评价光合作用样品的状态。
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9.发明申请公开(公告)号：US20070224659A1
公开(公告)日：2007-09-27
申请号：US10583128
申请日：2004-12-16
CPC分类号： G01N21/6408 , G01N21/6486 , G01N2021/635
摘要： A biological growth inhibition factor assay method includes: a first step of mixing a photosynthetic sample, with an aqueous solution sample to prepare a test measurement solution, letting the test measurement solution stand, and then after illuminating light onto the test measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; a second step of mixing the photosynthetic sample with a standard sample, in which biological growth inhibition factors are not present, to prepare a standard measurement solution, letting the standard measurement solution stand, and then after illuminating light onto the standard measurement solution for a predetermined illumination time, measuring the light amount of the delayed fluorescence that is emitted; and a third step of computing assay values based on the light amounts of delayed fluorescence, respectively measured in the first step and the second step, and determining a comparison value of the assay values to assay biological growth inhibition factors. A biological growth inhibition factor assay method that enables analysis of a wide range of inhibition factors in a short time is thereby realized.
摘要翻译： 生物生长抑制因子测定方法包括：将光合作用样品与水溶液样品混合以制备测试测量溶液的第一步骤，使测试测量溶液静置，然后将光照射到测试测量溶液上以达到预定的 照射时间，测量发射的延迟荧光的光量; 将光合作用样品与其中不存在生物生长抑制因子的标准样品混合以制备标准测量溶液，使标准测量溶液静置，然后将光照射到标准测量溶液上达预定的第二步骤 照射时间，测量发射的延迟荧光的光量; 以及分别基于第一步骤和第二步骤中测量的延迟荧光的光量来计算测定值的第三步骤，以及测定测定值以测定生物生长抑制因子的比较值。 从而实现了能够在短时间内分析广泛的抑制因子的生物生长抑制因子测定方法。
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