公开(公告)号：US5837839A

公开(公告)日：1998-11-17

申请号：US733825

申请日：1996-10-18

申请人： Matthew John Toth ,  Leslie Robin Huwyler

发明人： Matthew John Toth ,  Leslie Robin Huwyler

IPC分类号： C12N9/88 ,  C12N15/60 ,  C12N15/70 ,  G01N33/53

CPC分类号： C12Y401/01033 ,  C12N9/88

摘要： We have cloned and expressed the cDNA for human liver mevalonate pyrophosphate decarboxylase, an enzyme of the cholesterol synthesis pathway. This cDNA encodes a 400 amino acid protein (43 kDa) which resembles the coding sequence of an unidentified and incomplete open reading frame adjacent to the COQ2 gene of Saccharomyces cerevisiae. We also found an expression sequence tag clone made from human infant brain cDNA to be almost identical to our cDNA sequence.

摘要翻译： 我们克隆并表达了人肝磷酸焦磷酸脱羧酶（一种胆固醇合成途径的酶）的cDNA。 该cDNA编码400个氨基酸的蛋白质（43kDa），其类似于与酿酒酵母的COQ2基因相邻的未鉴定和不完整的开放阅读框的编码序列。 我们还发现由人类婴儿脑cDNA制成的表达序列标签克隆与我们的cDNA序列几乎相同。