公开(公告)号：US5981213A

公开(公告)日：1999-11-09

申请号：US450351

申请日：1995-05-25

申请人： Eric J. Hansen ,  Merja E. Helminen ,  Isobel Maciver

发明人： Eric J. Hansen ,  Merja E. Helminen ,  Isobel Maciver

IPC分类号： A61K38/00 ,  A61K39/00 ,  A61P31/04 ,  C07K14/21 ,  C07K16/12 ,  C12P21/08 ,  C12P21/06 ,  C07H21/02 ,  C12N1/12 ,  C12N15/00

CPC分类号： C07K16/1203 ,  C07K14/212 ,  C07K16/1214 ,  C07K16/1217 ,  A61K2039/505 ,  A61K38/00 ,  A61K39/00

摘要： The present disclosure relates to Moraxella catarrhalis outer membrane vesicle (OMV) compositions, to selected antigenic proteins from the outer membranes of M. catarrhalis which have a variety of useful properties, and to monoclonal antibodies against these proteins. Particular "Outer Membrane Proteins" (OMPs) of the invention are characterized as having molecular weights of about 30 kD, 80 kD (also termed CopB protein) and between about 200 and 700 kD (HMWP antigen). Passive immunization with monoclonal antibodies directed against these proteins confers protection against homologous and heterologous Moraxella catarrhalis strains in animal models, and active immunization with outer membrane vesicles also enhances pulmonary clearance of distinct M. catarrhalis strains. This demonstrates both the utility of antibodies in conferring passive immunity and the usefulness of OMPs, or variants thereof, in the preparation of vaccines. Also disclosed are DNA segments encoding these OMPs, methods for preparing the antigens, or variants, through the application of recombinant DNA techniques, as well as diagnostic methods and related embodiments.

摘要翻译： 本公开内容涉及卡他莫拉菌外膜囊泡（OMV）组合物，具有多种有用特性的卡他莫拉胶囊的外膜的选定抗原蛋白质以及针对这些蛋白质的单克隆抗体。 本发明的特定的“外膜蛋白”（OMP）的特征在于具有约30kD，80kD（也称为CopB蛋白）和约200至700kD（HMWP抗原）的分子量。 用针对这些蛋白质的单克隆抗体的被动免疫赋予动物模型中的同源和异源卡他莫拉菌株的保护，并且与外膜囊泡的主动免疫也增强了不同的卡他莫拉菌株的肺清除。 这证明了抗体在赋予被动免疫中的效用以及OMP或其变体在制备疫苗中的用途。 还公开了编码这些OMP的DNA片段，通过应用重组DNA技术制备抗原或变体的方法，以及诊断方法和相关实施方案。