Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
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    发明申请
    Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process 审中-公开
    在核酸扩增过程中使用错误检测和/或错误校正条形码的方法和组合

    公开(公告)号:US20100323348A1

    公开(公告)日:2010-12-23

    申请号:US12693612

    申请日:2010-01-26

    IPC分类号: C12Q1/68 C07H21/00

    摘要: The present invention provides methods and compositions for detecting and correcting errors in nucleic acid amplification processes, and methods for using the same. In particular, barcode amplification errors are detected and corrected such that integrity in sample assignment is maintained. The methods are compatible with high throughput sequencing techniques as some of the barcodes are based upon Hamming codes, thereby allowing self-correction for single bit errors. Some methods and compositions of the invention allow characterization (e.g., sequencing) of a plurality of nucleic acid samples simultaneously within a single sequencing reaction.

    摘要翻译: 本发明提供了用于检测和校正核酸扩增过程中的错误的方法和组合物及其使用方法。 特别地,检测和校正条形码放大误差,使得保持样本分配的完整性。 这些方法与高通量测序技术兼容,因为一些条形码基于汉明码,从而允许对单个位错误进行自校正。 本发明的一些方法和组合物允许在单个测序反应中同时对多个核酸样品进行表征(例如测序)。