公开(公告)号：US20090075834A1

公开(公告)日：2009-03-19

申请号：US12027239

申请日：2008-02-06

申请人： Sharon A. Doyle ,  Michael B. Murphy

发明人： Sharon A. Doyle ,  Michael B. Murphy

IPC分类号： C40B30/04

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12N2330/30 ,  C12Q1/6811

摘要： The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.

摘要翻译： 本方法是改进的体外选择方案，其依赖于DNA适配体生产的磁性分离，其相对容易且可扩展，而不需要昂贵的机器人。 还描述了通过该方法选择的适配体以高亲和力和特异性识别和结合其靶蛋白的能力，并详细描述了其在多种测定中的用途。 使用所述方法选择特异性TTF1和His6适体，并且显示可用于酶联试验，Western印迹和亲和纯化。

公开(公告)号：US06345306B1

公开(公告)日：2002-02-05

申请号：US09305647

申请日：1999-05-05

申请人： Gregory Richard Hintermeister ,  Gregory S. Hurlebaus ,  Erik Duane Lindberg ,  Robert Anthony Mahlik ,  Michael B. Murphy

发明人： Gregory Richard Hintermeister ,  Gregory S. Hurlebaus ,  Erik Duane Lindberg ,  Robert Anthony Mahlik ,  Michael B. Murphy

IPC分类号： G06F1516

CPC分类号： H04L63/08

摘要： An apparatus and method for creating packages and for transferring packages between computer systems provides a graphical user interface that allows a user to define various attributes for a package. Logical packages may be defined, which specify package elements to include in the package. Physical packages may also be defined, which contain a copy of the package elements specified in the logical package. Once a user defines a logical package, the logical package may be transferred to an endpoint system. The endpoint system reads the logical package, and from the parameters in the logical package determines where to retrieve the package elements and what other actions are appropriate while retrieving the package elements and constructing a physical package. In this manner, the endpoint system retrieves the package elements as required. The preferred embodiment of the present invention defines a packager using an object oriented framework mechanism that defines both core and extensible functions. Core functions of the framework mechanism cannot be modified by a user, while extensible functions in the framework mechanism are extended by a user to define a run-time package application. By providing an object oriented package framework mechanism, a developer can quickly and efficiently generate new package definitions and new features on the graphical user interface used to define a package.

摘要翻译： 用于创建包和用于在计算机系统之间传送包的装置和方法提供允许用户定义包的各种属性的图形用户界面。 可以定义逻辑包，指定要在包中包含的包元素。 还可以定义物理包，其中包含逻辑包中指定的包元素的副本。 一旦用户定义了逻辑包，则逻辑包可以被传送到端点系统。 端点系统读取逻辑包，并且从逻辑包中的参数确定在何处检索包元素，以及在检索包元素并构建物理包时还需要采取哪些其他操作。 以这种方式，端点系统根据需要检索包元素。 本发明的优选实施例定义了使用定义了核心和可扩展功能的面向对象的框架机制的包装机。 框架机制的核心功能不能被用户修改，而框架机制中的可扩展功能由用户进行扩展，以定义运行时包应用程序。 通过提供面向对象的包框架机制，开发人员可以在用于定义包的图形用户界面上快速有效地生成新的包定义和新功能。

公开(公告)号：US08105982B2

公开(公告)日：2012-01-31

申请号：US12027239

申请日：2008-02-06

申请人： Sharon A. Doyle ,  Michael B. Murphy

发明人： Sharon A. Doyle ,  Michael B. Murphy

IPC分类号： C07H21/02 ,  C40B20/04 ,  C40B30/04

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12N2330/30 ,  C12Q1/6811

摘要： The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.

摘要翻译： 本方法是改进的体外选择方案，其依赖于DNA适配体生产的磁性分离，其相对容易且可扩展，而不需要昂贵的机器人。 还描述了通过该方法选择的适配体以高亲和力和特异性识别和结合其靶蛋白的能力，并详细描述了其在多种测定中的用途。 使用所述方法选择特异性TTF1和His6适体，并且显示可用于酶联试验，Western印迹和亲和纯化。

公开(公告)号：US20100155349A1

公开(公告)日：2010-06-24

申请号：US12340459

申请日：2008-12-19

申请人： Michael B. Murphy

发明人： Michael B. Murphy

IPC分类号： A47F5/01

CPC分类号： B62B3/02 ,  B62B2202/52 ,  B62B2204/04

摘要： A boxed caddy or pallet suitable for quick installation and removal on top of a rolling ice chest or similar hexahedral appliance, into to which goods can be piled for transportation to a beach or picnic site. The caddy folds flat for convenient storing into a car trunk. The multi-railed sides of the caddy are telescopically adjustable. Brackets projecting downwardly from the lower edges of the sides come to rest against the walls of the appliance, thus providing bi-directional horizontal stability. Adjustable stabilization straps can conveniently be secured between the caddy and appliance. The caddy has a removable top cover and can double as a small table.

摘要翻译： 适用于在冰箱或类似的六面体设备顶部快速安装和拆卸的盒装小球或托盘，货物可以堆放在沙滩或野餐场所。 球童折叠平坦，方便存放在汽车后备箱中。 球杆的多栏杆可伸缩调节。 从侧面的下边缘向下突出的托架靠在设备的墙壁上，从而提供双向的水平稳定性。 可调节的稳定带可以方便地保证在球童和器具之间。 球童有一个可拆卸的顶盖，可以作为一个小桌子加倍。

公开(公告)号：US06640252B2

公开(公告)日：2003-10-28

申请号：US10037201

申请日：2002-01-04

申请人： Gregory Richard Hintermeister ,  Gregory S. Hurlebaus ,  Erik Duane Lindberg ,  Robert Anthony Mahlik ,  Michael B. Murphy

发明人： Gregory Richard Hintermeister ,  Gregory S. Hurlebaus ,  Erik Duane Lindberg ,  Robert Anthony Mahlik ,  Michael B. Murphy

IPC分类号： G06F1516

CPC分类号： H04L63/08

摘要： An apparatus and method for creating packages and for transferring packages between computer systems provides a graphical user interface that allows a user to define various attributes for a package. Logical packages may be defined, which specify package elements to include in the package. Physical packages may also be defined, which contain a copy of the package elements specified in the logical package. Once a user defines a logical package, the logical package may be transferred to an endpoint system. The endpoint system reads the logical package, and from the parameters in the logical package determines where to retrieve the package elements and what other actions are appropriate while retrieving the package elements and constructing a physical package. In this manner, the endpoint system retrieves the package elements as required. The preferred embodiment of the present invention defines a packager using an object oriented framework mechanism that defines both core and extensible functions. Core functions of the framework mechanism cannot be modified by a user, while extensible functions in the framework mechanism are extended by a user to define a run-time package application. By providing an object oriented package framework mechanism, a developer can quickly and efficiently generate new package definitions and new features on the graphical user interface used to define a package.

公开(公告)号：US06428380B1

公开(公告)日：2002-08-06

申请号：US09503454

申请日：2000-02-14

申请人： Michael B. Murphy

发明人： Michael B. Murphy

IPC分类号： A63H2700

CPC分类号： A63H27/00 ,  Y10T156/1033 ,  Y10T156/1036 ,  Y10T428/1328

摘要： A model airplane includes a main body component, a wing component, a horizontal stabilizer component, and a vertical stabilizer component. At least some of the components are fabricated of a shell of lightweight wood with spaces therebetween. A sheet of lightweight plastic material covers each of the components. The material is wrapped around the components with an individual sheet for the components. The plastic material is lightweight with a thickness of between about 0.65 and 0.85 mils and has the ability when heated to contract to about 25 percent of its original size.

摘要翻译： 模型飞机包括主体部件，翼部件，水平稳定器部件和垂直稳定器部件。 至少一些部件由轻质木材的外壳制成，其间具有间隙。 一片轻质的塑料材料覆盖每个组件。 材料用组件的单个片材包裹在组件周围。 塑料材料重量轻，厚度介于约0.65至0.85密耳之间，并具有加热收缩至其原始尺寸的约25％的能力。

公开(公告)号：US07329742B2

公开(公告)日：2008-02-12

申请号：US10934856

申请日：2004-09-03

申请人： Sharon A. Doyle ,  Michael B. Murphy

发明人： Sharon A. Doyle ,  Michael B. Murphy

IPC分类号： C07H21/02 ,  C12Q1/68 ,  C12M1/00 ,  G01N33/551

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12N2330/30 ,  C12Q1/6811

摘要： The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.

摘要翻译： 本方法是改进的体外选择方案，其依赖于DNA适配体生产的磁性分离，其相对容易且可扩展，而不需要昂贵的机器人。 还描述了通过该方法选择的适配体以高亲和力和特异性识别和结合其靶蛋白的能力，并详细描述了其在多种测定中的用途。 使用所述方法选择特异性TTF1和His6适体，并且显示可用于酶联试验，Western印迹和亲和纯化。