公开(公告)号：US20100113292A1

公开(公告)日：2010-05-06

申请号：US12557358

申请日：2009-09-10

申请人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

发明人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

IPC分类号： C40B30/02 ,  C40B50/02

CPC分类号： C12N15/113 ,  C07H21/00 ,  C12Q1/6811

摘要： Methods for obtaining antisense oligonucleotides with activity against a desired target are provided. Methods of identifying oligonucleotide sequence motifs which are predictive of antisense oligonucleotide activity are provided, as are motifs identified according to this method. Methods of selecting effective antisense oligonucleotide sequences and effective antisense target sequences are provided, as are sequences selected according to these methods. In other methods of the invention, oligonucleotides are designed to hybridize to target sequences containing one or more activity-enhancing motifs. Antisense oligonucleotides designed according to these methods are also provided.

摘要翻译： 提供了获得具有针对所需靶标的活性的反义寡核苷酸的方法。 提供了鉴定预测反义寡核苷酸活性的寡核苷酸序列基序的方法，以及根据该方法鉴定的基序。 提供了选择有效的反义寡核苷酸序列和有效的反义靶序列的方法，以及根据这些方法选择的序列。 在本发明的其它方法中，设计寡核苷酸与含有一种或多种活性增强基序的靶序列杂交。 还提供了根据这些方法设计的反义寡核苷酸。

公开(公告)号：US07592440B2

公开(公告)日：2009-09-22

申请号：US10686317

申请日：2003-10-15

申请人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

发明人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

IPC分类号： C07H21/04 ,  C07H21/00 ,  C40B30/02 ,  C40B30/10 ,  C40B40/06

CPC分类号： C12N15/113 ,  C07H21/00 ,  C12Q1/6811

摘要： Methods for obtaining antisense oligonucleotides with activity against a desired target are provided. Methods of identifying oligonucleotide sequence motifs which are predictive of antisense oligonucleotide activity are provided, as are motifs identified according to this method. Methods of selecting effective antisense oligonucleotide sequences and effective antisense target sequences are provided, as are sequences selected according to these methods. In other methods of the invention, oligonucleotides are designed to hybridize to target sequences containing one or more activity-enhancing motifs. Antisense oligonucleotides designed according to these methods are also provided.

摘要翻译： 提供了获得具有针对所需靶标的活性的反义寡核苷酸的方法。 提供了鉴定预测反义寡核苷酸活性的寡核苷酸序列基序的方法，以及根据该方法鉴定的基序。 提供了选择有效的反义寡核苷酸序列和有效的反义靶序列的方法，以及根据这些方法选择的序列。 在本发明的其它方法中，设计寡核苷酸与含有一种或多种活性增强基序的靶序列杂交。 还提供了根据这些方法设计的反义寡核苷酸。

公开(公告)号：US08202982B2

公开(公告)日：2012-06-19

申请号：US12557358

申请日：2009-09-10

申请人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

发明人： Susan M. Freier ,  Olga Matveeva ,  Alexander Tsodikov ,  Michael C. Giddings ,  Jacqueline R. Wyatt

IPC分类号： C07H21/00 ,  C07H21/04 ,  A61K31/70

CPC分类号： C12N15/113 ,  C07H21/00 ,  C12Q1/6811

摘要： Methods for obtaining antisense oligonucleotides with activity against a desired target are provided. Methods of identifying oligonucleotide sequence motifs which are predictive of antisense oligonucleotide activity are provided, as are motifs identified according to this method. Methods of selecting effective antisense oligonucleotide sequences and effective antisense target sequences are provided, as are sequences selected according to these methods. In other methods of the invention, oligonucleotides are designed to hybridize to target sequences containing one or more activity-enhancing motifs. Antisense oligonucleotides designed according to these methods are also provided.

摘要翻译： 提供了获得具有针对所需靶标的活性的反义寡核苷酸的方法。 提供了鉴定预测反义寡核苷酸活性的寡核苷酸序列基序的方法，以及根据该方法鉴定的基序。 提供了选择有效的反义寡核苷酸序列和有效的反义靶序列的方法，以及根据这些方法选择的序列。 在本发明的其它方法中，设计寡核苷酸与含有一种或多种活性增强基序的靶序列杂交。 还提供了根据这些方法设计的反义寡核苷酸。