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公开(公告)号：US6090622A
公开(公告)日：2000-07-18
申请号：US829372
申请日：1997-03-31
CPC分类号： G01N33/56966 , C12N5/0603 , C12N5/0611 , G01N33/689 , C12N2501/01 , C12N2501/115 , C12N2501/235 , C12N2502/13
摘要： Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
摘要翻译： 从后囊胚人类胚胎提取的原始生殖细胞，例如从8-11周的LMP人胚胎的性腺中提取。 原始生殖细胞在长期培养（30天以上）中培养，导致类似胚胎干细胞形态和多能性的细胞。 细胞在培养物中保持数月，可以使用转基因技术进行遗传操作以插入异源遗传物质。
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公开(公告)号：US06245566B1
公开(公告)日：2001-06-12
申请号：US09052772
申请日：1998-03-31
IPC分类号： C12N502
CPC分类号： C12N5/0611 , C12N5/0603 , C12N2501/01 , C12N2501/115 , C12N2501/235 , C12N2502/1323 , G01N33/56966 , G01N33/689
摘要： Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
摘要翻译： 公开了从人胚胎组织分离的原始生殖细胞，例如来自人胚胎的性腺脊髓。 培养原始生殖细胞，产生类似胚胎干细胞或胚胎生殖细胞的形态和多能性的细胞。 细胞在培养物中保持数月，可以使用转基因技术进行遗传操作以插入异源遗传物质。
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公开(公告)号：US06562619B1
公开(公告)日：2003-05-13
申请号：US09553640
申请日：2000-04-20
IPC分类号： C12N508
CPC分类号： C12N5/0611 , C12N5/0603 , C12N2501/01 , C12N2501/115 , C12N2501/235 , C12N2502/1323 , G01N33/56966 , G01N33/689
摘要： Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
摘要翻译： 公开了从人胚胎组织分离的原始生殖细胞，例如来自人胚胎的性腺脊髓。 培养原始生殖细胞，产生类似胚胎干细胞或胚胎生殖细胞的形态和多能性的细胞。 细胞在培养物中保持数月，可以使用转基因技术进行遗传操作以插入异源遗传物质。
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公开(公告)号：US06331406B1
公开(公告)日：2001-12-18
申请号：US08989744
申请日：1997-12-12
IPC分类号： G01N33567
CPC分类号： C12N5/0611 , C12N5/0603 , C12N2501/01 , C12N2501/115 , C12N2501/235 , C12N2502/1323 , G01N33/56966 , G01N33/689
摘要： Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
摘要翻译： 从后囊胚人类胚胎提取的原始生殖细胞，例如从8-11周的LMP人胚胎的性腺中提取。 原始生殖细胞在长期培养（30天以上）中培养，导致类似胚胎干细胞形态和多能性的细胞。 细胞在培养物中保持数月，可以使用转基因技术进行遗传操作以插入异源遗传物质。
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