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    ISOLATED NAIVE PLURIPOTENT STEM CELLS AND METHODS OF GENERATING SAME
    8.
    发明申请
    ISOLATED NAIVE PLURIPOTENT STEM CELLS AND METHODS OF GENERATING SAME 审中-公开
    分离的内源性干细胞及其产生方法

    公开(公告)号：US20140315301A1

    公开(公告)日：2014-10-23

    申请号：US14259997

    申请日：2014-04-23

    申请人： Yeda Research and Development Co. Ltd.

    发明人： Yaqub HANNA ,  Noa Novershtern ,  Yoach Rais

    IPC分类号： C12N5/0735 ,  C12N5/074

    CPC分类号： C12N5/0611 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/42 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/999

    摘要： Provided is an isolated human naive pluripotent stem cell (PSC), wherein: (i) when the naive PSC is a female PSC, then said naive female PSC has two unmethylated alleles of an X-inactive specific transcript (XIST) gene; and (ii) when said naive PSC is a male PSC, then said naive male PSC has an unmethylated allele of said XIST gene. Also provided is a culture medium which comprises an ERK1/2 inhibitor, a GSK3beta inhibitor, a p38 inhibitor, a JNK inhibitor, a STAT3 activator and at least one agent selected from the group consisting of: bFGF, TGFbeta 1, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor; or at least agent selected from the group consisting of: a TGFR inhibitor, a FGFR inhibitor, a PKC inhibitor, a ROCK inhibitor and a NOTCH inhibitor.

    摘要翻译： 提供了一种分离的人类天真多能干细胞（PSC），其中：（i）当初始PSC是女性PSC时，所述初始女性PSC具有X-非活性特异性转录物（XIST）基因的两个未甲基化等位基因; 和（ii）当所述初始PSC是雄性PSC时，所述初始雄性PSC具有所述XIST基因的未甲基化等位基因。 还提供了包含ERK1 / 2抑制剂，GSK3β抑制剂，p38抑制剂，JNK抑制剂，STAT3活化剂和至少一种选自以下的药剂的培养基：bFGF，TGFβ1，PKC抑制剂， ROCK抑制剂和NOTCH抑制剂; 或至少选自TGFR抑制剂，FGFR抑制剂，PKC抑制剂，ROCK抑制剂和NOTCH抑制剂的药剂。

    Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations
    10.
    发明授权
    Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations 有权
    用于分选到具有X或Y染色体的富集群体的精子悬浮液

    公开(公告)号：US07838210B2

    公开(公告)日：2010-11-23

    申请号：US11092313

    申请日：2005-03-29

    申请人： Cindy L. Ludwig ,  Kathleen S. Crowley ,  Charles N. Graves

    发明人： Cindy L. Ludwig ,  Kathleen S. Crowley ,  Charles N. Graves

    IPC分类号： A01N1/02

    CPC分类号： A61K35/52 ,  A01N1/021 ,  A01N1/0226 ,  C12N5/061 ,  C12N5/0611 ,  C12N2500/05 ,  G01N1/30

    摘要： Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.

    摘要翻译： 公开了包含运动性抑制剂的精子细胞悬液。 这种悬浮液中所含的细胞倾向于具有更大的耐受通常与将精子细胞分选为富含性别的群体有关的各种方法步骤的能力，从而导致具有增加的活力或活力精子数量的分类后组合物。 还公开了形成这种细胞悬浮液的方法以及染色精子细胞的方法。