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公开(公告)号：US08436142B2
公开(公告)日：2013-05-07
申请号：US13122487
申请日：2009-10-02
CPC分类号： C07K14/535 , C07K2319/02
摘要： A G-CSF precursor comprising a signal peptide and a G-CSF peptide, wherein the signal peptide has the sequence of the human wild-type signal peptide of the human G-CSF/b molecule with at least one of the following mutations: deletion of Glu29, insertion of Glu26, substitution Lys11Leu, substitution His21Phe, and substitution Gln28Leu.
摘要翻译： 包含信号肽和G-CSF肽的G-CSF前体，其中信号肽具有人G-CSF / b分子的人野生型信号肽的序列，具有以下突变中的至少一个：缺失 Glu29，Glu26插入，Lys11Leu取代，His21Phe取代，Gln28Leu取代。
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公开(公告)号：US20110294989A1
公开(公告)日：2011-12-01
申请号：US13122487
申请日：2009-10-02
IPC分类号： C07K14/535 , C12P21/02 , C12N5/10 , C07H21/00 , C12N15/63
CPC分类号： C07K14/535 , C07K2319/02
摘要： A G-CSF precursor comprising a signal peptide and a G-CSF peptide, wherein the signal peptide has the sequence of the human wild-type signal peptide of the human G-CSF/b molecule with at least one of the following mutations: deletion of Glu29, insertion of Glu26, substitution Lys11Leu, substitution His21Phe, and substitution Glu28Leu.
摘要翻译： 包含信号肽和G-CSF肽的G-CSF前体，其中信号肽具有人G-CSF / b分子的人野生型信号肽的序列，具有以下突变中的至少一个：缺失 Glu29插入，Glu26插入，Lys11Leu取代，His21Phe取代，Glu28Leu取代。
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公开(公告)号：US07572619B2
公开(公告)日：2009-08-11
申请号：US10239498
申请日：2001-03-21
CPC分类号： C12N9/6437 , A61K38/00 , A61K48/00 , C07K14/745 , C07K14/755 , C12N9/644 , C12N9/647 , C12Y304/21021 , C12Y304/21022
摘要： The present invention relates to an improved method for the production of recombinant human blood clotting factors, in particular of factor VIII and factor IX. An immortalized human cell line can be used to stably express viral transcription activator proteins and carrying a vector having a promoter functionally linked to a DNA sequence coding for a blood coagulating factor, provided that said promoter is not a viral promoter which is stimulated by said viral transcription activator proteins. The invention further relates to an immortalized human cell line carrying said vector, factor VIII muteins particularly suitable for the above production method; pharmaceutical compositions comprising such factor VIII muteins, and the use of such factor VIII muteins for preparing a medicament for treating hemophilia.
摘要翻译： 本发明涉及生产重组人凝血因子，特别是因子VIII和因子IX的改进方法。 可以使用永生化的人细胞系来稳定表达病毒转录激活蛋白并携带具有与编码凝血因子的DNA序列功能性连接的启动子的载体，前提是所述启动子不是所述病毒刺激的病毒启动子 转录激活蛋白。 本发明还涉及携带所述载体的永生化人细胞系，特别适用于上述制备方法的因子VIII突变蛋白; 包含这种因子VIII突变蛋白的药物组合物，以及使用这种因子VIII突变蛋白来制备治疗血友病的药物。
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