公开(公告)号：US5250432A

公开(公告)日：1993-10-05

申请号：US589495

申请日：1990-09-27

申请人： Michiyuki Takashiki ,  Kimihiko Hamamoto ,  Kenji Ishimaru

发明人： Michiyuki Takashiki ,  Kimihiko Hamamoto ,  Kenji Ishimaru

IPC分类号： C12M1/26 ,  C12M3/02 ,  C12N5/02 ,  C12N5/06 ,  C12N5/12 ,  C12N5/16

CPC分类号： C12M47/02 ,  B04B2005/0457 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/33 ,  Y10S435/813

摘要： A method of culturing animal cells, which comprises (A) subjecting living animal cells to suspension culture in a culture tank, (B) withdrawing a portion of a suspension culture fluid containing the living animal cells from the culture tank, (C) supplying the withdrawn suspension culture fluid to a centrifugal separating device which is operated under specific conditions and separating the living animal cells from the suspension culture fluid, (D) withdrawing the separated living animal cells from the centrifugal separating device, and (E) recycling at least a portion of the withdrawn living animal cells to the culture tank for the step (A).

摘要翻译： 一种培养动物细胞的方法，其特征在于：（A）使活体动物细胞在培养槽中进行悬浮培养，（B）从培养槽中取出含有活性动物细胞的悬浮培养液的一部分，（C） 将脱出的悬浮培养液输送到在特定条件下运转并将活体动物细胞与悬浮培养液分离的离心分离装置，（D）从离心分离装置取出分离的活体动物细胞，（E）将至少 取出的活体动物细胞的一部分用于步骤（A）的培养罐。