公开(公告)号：US20240318133A1

公开(公告)日：2024-09-26

申请号：US18731343

申请日：2024-06-02

申请人： Upside Foods, Inc.

发明人： Kalle Lukyan JOHNSON ,  Patricia R. BENTON ,  Cameron Gentry COPELAND ,  Sukhdeep Singh DHADWAR ,  Meri Teresa FIRPO ,  Marie Elizabeth GIBBONS ,  Stephen K. HSU ,  Robert SIERRA ,  Kevin John KAYSER

IPC分类号： C12N5/071

CPC分类号： C12N5/0602 ,  C12N2500/14 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33

摘要： Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.

公开(公告)号：US20240018478A1

公开(公告)日：2024-01-18

申请号：US18256230

申请日：2021-12-07

申请人： GENOME BIOLOGICS UG

发明人： Duc Minh PHAM ,  Jaya KRISHNAN ,  Stefanie DIMMELER

IPC分类号： C12N5/077

CPC分类号： C12N5/0657 ,  C12N2501/115 ,  C12N2506/45 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2503/02 ,  C12N2501/40 ,  C12N2501/155 ,  C12N2513/00 ,  C12N2501/16

摘要： The present invention relates to a preparation method for a self-organized cardiac organoid. The invention also relates to a cardiac tissue organoid obtained by the method and to its use in regenerative medicine, as a tissue implant, or in drug screening.

公开(公告)号：US20240009249A1

公开(公告)日：2024-01-11

申请号：US18038630

申请日：2021-11-24

申请人： Flagship Pioneering, Inc.

发明人： Alexandra Sophie DE BOER ,  Le Phuan Lizzie NGO ,  Avak KAHVEJIAN ,  Jordan LEWANDOWSKI

IPC分类号： A61K35/35 ,  C12N5/077 ,  C12N5/0775 ,  A61K35/28 ,  A61K38/18 ,  A61P43/00

CPC分类号： A61K35/35 ,  C12N5/0653 ,  C12Y114/16001 ,  C12N5/0665 ,  A61K35/28 ,  A61K38/1816 ,  A61P43/00 ,  C12N2501/2303 ,  C12N2501/22 ,  C12N2501/33 ,  C12N2501/26 ,  C12N2506/1369

摘要： Disclosed herein are compositions comprising adipogenic cells that are useful for the treatment, prevention, or amelioration of diseases or disorders.

公开(公告)号：US20230272489A1

公开(公告)日：2023-08-31

申请号：US18300253

申请日：2023-04-13

申请人： Tempus Labs, Inc.

发明人： Ameen Salahudeen ,  Verónica Sánchez Freire ,  Brandon L. Mapes

IPC分类号： C12Q1/6886 ,  C12N5/071 ,  G16H50/30 ,  C12M1/32 ,  G01N33/50 ,  G16B20/20 ,  C12N5/09 ,  C12Q1/02 ,  C12Q1/6806 ,  C12Q1/6816

CPC分类号： C12Q1/6886 ,  C12N5/0691 ,  G16H50/30 ,  C12M23/12 ,  G01N33/5091 ,  G16B20/20 ,  C12N5/0693 ,  C12Q1/025 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12N2500/25 ,  C12N2527/00 ,  C12N2521/10 ,  C12N2509/10 ,  C12N2501/117 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/999 ,  C12N2501/998 ,  C12N2533/54 ,  C12N2533/52 ,  C12N2533/90 ,  C12N2501/33 ,  C12Q1/6844

摘要： Provided herein are novel organoid culture media, organoid culture systems, and methods of culturing tumor organoids using the subject organoid culture media. Also provided herein are tumor organoids developed using such organoid culture systems, methods for assessing the clonal diversity of the tumor organoids, and methods for using such tumor organoids, for example, for tumor modelling and drug development applications. In particular embodiments, the tumor organoid culture media provided herein is substantially free of R-spondins (e.g., R-spondin1).

公开(公告)号：US20190233798A1

公开(公告)日：2019-08-01

申请号：US15945718

申请日：2018-04-04

申请人： Georgetown University

发明人： Richard Schlegel ,  Xuefeng Liu

IPC分类号： C12N5/071 ,  G01N33/50 ,  C12Q1/6883

CPC分类号： C12N5/0683 ,  C12N5/0625 ,  C12N5/0631 ,  C12N5/067 ,  C12N5/0688 ,  C12N2500/05 ,  C12N2500/40 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2502/13 ,  C12N2502/1323 ,  C12N2503/02 ,  C12N2510/04 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/5091

摘要： The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.

公开(公告)号：US20190194608A1

公开(公告)日：2019-06-27

申请号：US16193198

申请日：2018-11-16

申请人： The Jackson Laboratory

发明人： Wa Xian

IPC分类号： C12N5/074 ,  A61K35/39 ,  A61K35/22 ,  C12N5/0797 ,  C12N5/071 ,  C12N5/095 ,  G01N33/50 ,  A61K35/42 ,  A61K35/413 ,  A61K35/30 ,  A61K35/48 ,  A61K35/36 ,  A61K35/407 ,  A61K35/38

CPC分类号： C12N5/0607 ,  A61K35/22 ,  A61K35/30 ,  A61K35/36 ,  A61K35/38 ,  A61K35/39 ,  A61K35/407 ,  A61K35/413 ,  A61K35/42 ,  A61K35/48 ,  C12N5/0623 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0685 ,  C12N5/0689 ,  C12N5/0695 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2533/52 ,  G01N33/5073

摘要： The invention described herein relates to methods of isolating non-embryonic stem cell, e.g., adult stem cell, from a non-embryonic tissue, e.g., an adult tissue or organ. Non-embryonic stem cells (e.g., adult stem cells) thus isolated from the various tissues or organs can self-renew or propagate indefinitely in vitro, are multipotent and can differentiate into the various differentiated cell types normally found within the tissue or organ from which the stem cells are isolated. In addition, the isolated stem cells can be propagated through clonal expansion of a single isolated stem cell, to produce a clone of which at least about 40%, 70%, or 90% or more cells within the clone can be further passaged as single cell originated clones.

公开(公告)号：US10017735B2

公开(公告)日：2018-07-10

申请号：US14415972

申请日：2013-07-18

申请人： SINGAPORE HEALTH SERVICES PTE LTD

发明人： Donald Tan ,  Jodhbir Mehta ,  Swee Lim Gary Peh

IPC分类号： C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0621 ,  C12N2500/24 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/62 ,  C12N2500/72 ,  C12N2500/80 ,  C12N2501/06 ,  C12N2501/10 ,  C12N2501/31 ,  C12N2501/33 ,  C12N2501/39

摘要： The invention relates to a dual-media approach for culturing isolated corneal endothelial cells. Isolated corneal endothelial cells are first contacted with a proliferative medium to propagate and/or expand the endothelial cells followed by a maintenance medium to preserve the morphology and/or characteristics of the corneal endothelial cells. The invention includes the proliferative medium and the maintenance medium and also a combination of the two medium.

公开(公告)号：US20180179492A1

公开(公告)日：2018-06-28

申请号：US15588964

申请日：2017-05-08

申请人： The Jackson Laboratory

发明人： Wa Xian

IPC分类号： C12N5/074 ,  C12N5/0797 ,  A61K35/42 ,  A61K35/48 ,  A61K35/22 ,  A61K35/30 ,  A61K35/38 ,  A61K35/39 ,  C12N5/095 ,  C12N5/071 ,  A61K35/407

CPC分类号： C12N5/0607 ,  A61K35/22 ,  A61K35/30 ,  A61K35/38 ,  A61K35/39 ,  A61K35/407 ,  A61K35/42 ,  A61K35/48 ,  C12N5/0623 ,  C12N5/0672 ,  C12N5/0676 ,  C12N5/0679 ,  C12N5/0685 ,  C12N5/0695 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2533/52

摘要： The invention described herein relates to methods of isolating non-embryonic stem cell, e.g., adult stem cell, from a non-embryonic tissue, e.g., an adult tissue or organ. Non-embryonic stem cells (e.g., adult stem cells) thus isolated from the various tissues or organs can self-renew or propagate indefinitely in vitro, are multipotent and can differentiate into the various differentiated cell types normally found within the tissue or organ from which the stem cells are isolated. In addition, the isolated stem cells can be propagated through clonal expansion of a single isolated stem cell, to produce a clone of which at least about 40%, 70%, or 90% or more cells within the clone can be further passaged as single cell originated clones.

公开(公告)号：US20170327558A1

公开(公告)日：2017-11-16

申请号：US15663912

申请日：2017-07-31

申请人： BRISTOL-MYERS SQUIBB COMPANY

发明人： Jun Tian ,  Michael Borys ,  Zhengjian Li ,  Nicholas Abuabsi ,  Angela Au ,  Nan-xin Qian ,  Xiao-ping Dai

IPC分类号： C07K14/705 ,  C12P21/02 ,  C12N5/00

CPC分类号： C07K14/70575 ,  C12N5/0018 ,  C12N2500/95 ,  C12N2501/105 ,  C12N2501/33 ,  C12N2510/02 ,  C12P21/02

摘要： The present invention describes methods and processes for the production of proteins by animal cell or mammalian cell culture. In one aspect, the methods comprise the growth of cells in a growth factor/protein/peptide free medium. In another aspect, the methods comprise the addition of growth factors during the production phase. The methods sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product.

公开(公告)号：US09777256B2

公开(公告)日：2017-10-03

申请号：US15024590

申请日：2014-09-24

申请人： UNIVERSITY OF MIAMI

发明人： Tan A. Ince

IPC分类号： C12N5/095 ,  C12N5/071 ,  C12N5/09

CPC分类号： C12N5/0631 ,  C12N5/0693 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2500/46 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/392

摘要： A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.