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公开(公告)号：US09574208B2
公开(公告)日：2017-02-21
申请号：US14129038
申请日：2012-06-19
申请人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：US09499837B2
公开(公告)日：2016-11-22
申请号：US13526912
申请日：2012-06-19
申请人： Dennis Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek A Lyznik , James Jefferson Smith
发明人： Dennis Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek A Lyznik , James Jefferson Smith
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：US20150184194A1
公开(公告)日：2015-07-02
申请号：US14129038
申请日：2012-06-19
申请人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：US20140020131A1
公开(公告)日：2014-01-16
申请号：US13526912
申请日：2012-06-19
申请人： Dennis L. Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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